延边地区朝鲜族12个X-STR基因座遗传多态性

发布时间：2018-01-23 03:01

本文关键词： X染色体短串联重复序列遗传多态性延边地区朝鲜族　出处：《延边大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:为了获取延边地区朝鲜族12个X-STR基因座(DXS7132、DXS7423、DXS8378、DXS10074、DXS10079、DXS10101、DXS10103、DXS10134、DXS10135、DXS10146、DXS10148和HPRTB)的等位基因频率分布、基因型频率分布、单倍型等相关群体遗传多态性指标,确认法医学应用价值。为中国朝鲜族群体的亲子鉴定、个人识别及群体遗传学研究提供基础资料。评估Investigator Argus X-12试剂盒在延边朝鲜族群体X-STR基因座研究中的实用效能方法:采集延边地区朝鲜族无关个332名(女176,男156)健康无关个体的静脉血样。采用 Chelex-100 试剂提取 DNA,应用 Investigator Argus X-12试剂盒(QIAGEN)使用手册中介绍的方法对12个X-STR基因座进行PCR扩增。用310基因分析仪进行毛细管电泳和等位基因的判读。用Power Stats Software软件进行数据统计分析,获取等位基因频率、杂合度(heterozygosity,H)、个体识别力(power of discrimination,PD)、多态性信息含量(polymorphism information content,PIC)等;用Genalex6.5软件对女性群体数据作基因型频率的统计,并且进行Hardy-Weinberg平衡吻合度(p-value)检验;对男性群体检测结果,用Arlequin软件进行单倍型多样性(haplotype diversity,HD)统计,用P0PTREE2软件进行群体遗传学数据的分析。结果:在延边朝鲜族12个X-STR基因座上分别检出6、6、7、19、9、18、25、4、20、9、5和23个等位基因,多态性信息含量分布为0.4306-0.9139;杂合度分布为0.5511-0.9318,除了 DXS7423之外均显示为较高的遗传特性。其中176名女性群体,观测杂合度均大于0.5511,多态信息含量均大于0.4306,个人识别力均大于0.6667,除了多等位基因的DXS10134,DXS10135,DXS10148基因座外其余9个基因型频率分布均符合Hardy-Weinberg平衡定律(P0.05);在男性群体,156名不同个体中检测到155种单倍型,其中最多见的单倍型为16-10-15-39-17-32-21-14-27-19-14-20,单倍型多样性为 0.9999。群体遗传学数据显示,延边朝鲜族与韩国人之间的遗传距离最小,为0.004,与中部克罗地亚人遗传距离最远,为0.042。结论:12个X-STR基因座除DXS7423均显示为较高的遗传统计数据,适合中国朝鲜族群体法医学鉴定和群体遗传学研究。本研究结果可为中国朝鲜族群体的亲子鉴定、个人识别及群体遗传学研究提供基础资料。Investigator Argus X-12试剂盒在中国延边地区朝鲜族人群12个X-STR基因座的遗传多态性研究中,具有较高的实际应用效能,该试剂盒可用于群体遗传学、法医学等相关领域
[Abstract]:Objective: to obtain 12 X-STR gene loci DXS7132DXS7423DXS8378DXS10074 DXS10079 from Korean nationality in Yanbian area. The allele frequency distribution of DXS10101 (DXS10103), DXS10134 (DXS10135), DXS10146 (DXS10148) and HPRTB. Genotypic frequency distribution, haplotype and other related population genetic polymorphism indicators, confirmed the value of forensic medicine, for Chinese Korean population paternity test. Provision of basic information for personal identification and population genetics research. Evaluation of Investigator Argus. The effectiveness of X-12 Kit in the study of X-STR Loci in Yanbian Korean population: 332 unrelated Korean population in Yanbian area (. Female 176. Chelex-100 reagent was used to extract DNA. Application of Investigator Argus X-12 Kit to QIAGEN. 12 X-STR loci were amplified by PCR using the method described in the manual. Capillary electrophoresis and allele interpretation using 310 gene analyzer. Power Stats. The data were analyzed by Software software. The frequency of alleles, heterozygosity of heterozygosity, and individual recognition power of discrimination were obtained. Polymorphic information content (information) and polymorphic information content (information); Genalex6.5 software was used to analyze the genotype frequency of female population data, and the Hardy-Weinberg equilibrium coincidence degree and p-valuetest were performed. The results of male population test were analyzed by Arlequin software. The population genetic data were analyzed by P0PTREE2 software. Results: the results showed that there were 6 X STR loci in 12 X-STR loci in Yanbian Korean nationality. The distribution of polymorphism information content was 0.4306-0.9139. The heterozygosity distribution was 0.5511-0.9318.The heterozygosity was higher than 0.5511 in 176 female populations except DXS7423. The polymorphic information content was greater than 0.4306, and the individual recognition power was higher than 0.6667, except for the polyallele DXS10134and DXS10135. The other 9 genotypes in the DXS10148 locus were all in accordance with the Hardy-Weinberg equilibrium law (P0.05A). 155 haplotypes were detected in 156 different individuals from male population. The most common haplotypes were 16-10-15-39-17-32-21-14-27-19-14-20. The diversity of haplotypes was 0.99999.The genetic data showed that the genetic distance between Korean and Korean was the smallest (0.004), and the genetic distance was the furthest from central Croat. Conclusion: all the 12 X-STR loci showed high genetic statistics except DXS7423. It is suitable for forensic identification and population genetics study of Korean population in China. The results of this study can be used for paternity identification of Korean population in China. Personal Identification and population Genetics Research provides basic data .Investigator Argus. The X-12 kit was used to study the genetic polymorphism of 12 X-STR loci in the Korean population in Yanbian area of China. The kit can be used in population genetics, forensic medicine and other related fields.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：D919

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