自主跑轮运动对幼龄小鼠学习记忆、海马神经发生及相关因子的影响

发布时间：2018-01-26 00:43

本文关键词： 自主跑轮运动神经发生神经细胞黏附因子(NCAM) 干细胞因子(SCF) 学习记忆胰岛素样生长因子(IGF-1) 血管内皮生长因子(VEGF)　出处：《华东师范大学》2012年硕士论文　论文类型：学位论文

【摘要】：研究目的：适宜的运动训练能够提高机体的学习记忆能力,但其具体机制目前还不是完全清楚。神经发生是指神经元的生成,其过程包括神经元细胞增殖、迁移、分化与存活等几个关键步骤,每个步骤都可能会影响到新生细胞和新生神经元的最终数目。海马内神经发生可能促进机体学习记忆能力的提高。目前,大量实验研究表明,运动训练能够促进海马神经发生,进而提高机体的学习记忆能力。NCAM与SCF是海马内与神经发生密切相关的因子,可以作为海马神经发生的标志性因子。本文采用Morris水迷宫及实时定量PCR的方法就8周自主跑轮运动对幼龄小鼠学习记忆及其与海马神经发生密切相关基因mRNA表达的影响进行研究,探讨神经发生在运动训练促进学习记忆能力方面的作用及意义。研究方法：经过筛选,选出12只小鼠作为研究对象,随机分成安静对照组(C组,n=6)和跑轮运动组(R组,n=6)。R组小鼠每天进行自主跑轮运动,连续8周。实验进行到第8周时,对全部小鼠进行Morris水迷宫实验测试,检测它们的空间学习与记忆能力。测试完成24小时后对全部小鼠采用断颈法处死,取出其海马组织冷冻、待测。用RT-PCR检测两组小鼠海马NCAM、SCF、IGF-1、 VEGF mRNA的表达水平。实验结果： (1)通过Morris水迷宫测试发现,同安静对照组小鼠相比,跑轮运动组小鼠在学习能力以及记忆测试中的表现更优秀。 (2)8周自主跑轮运动后,跑轮运动小鼠海马NCAM mRNA的表达均显著高于安静对照组小鼠(p0.05)。 (3)8周自主跑轮运动后,跑轮运动小鼠海马SCF mRNA的表达均显著高于安静对照组小鼠(p0.05)。 (4)8周自主跑轮运动后,跑轮运动组小鼠海马IGF-1mRNA的表达显著高于安静对照组小鼠(p0.01)。 (5)8周自主跑轮运动后,跑轮运动组小鼠海马VEGF mRNA的表达显著高于安静对照组小鼠(p0.05)。结论： (1)8周自主跑轮运动能够促进幼龄小鼠空间学习和记忆能力。 (2)8周自主跑轮运动后,跑轮运动组小鼠海马内NCAM和SCFmRNA表达的增加,推测跑轮运动组小鼠海马内神经发生多于安静组小鼠,提示八周自主跑轮运动可能促进小鼠海马神经发生,进而提示,8周自主跑轮运动对小鼠学习记忆功能的促进作用可能与海马神经发生有关。 (3)8周自主跑轮运动能促进小鼠海马内IGF-lmRNA的表达水平,提示8周自主跑轮运动可能通过提高小鼠海马内IGF-lmRNA的表达水平进而促进小鼠海马神经发生。 (4)8周自主跑轮运动能促进小鼠海马内VEGFmRNA的表达水平,提示8周自主跑轮运动可能通过提高小鼠海马内VEGFmRNA的表达水平进而促进小鼠海马神经发生。
[Abstract]:Objective: appropriate exercise training can improve the ability of learning and memory, but its specific mechanism is not completely clear. Neurogenesis refers to the formation of neurons, including neuronal cell proliferation. Migration, differentiation, survival and other key steps, each step may affect the final number of newborn cells and neurons. Hippocampal neurogenesis may promote the improvement of the ability of learning and memory. A large number of experimental studies have shown that exercise training can promote hippocampal neurogenesis and then improve the learning and memory ability of the body. NCAM and SCF are closely related to neurogenesis in the hippocampus. Morris water maze and real time quantitative PCR were used to study the learning and memory of young mice and their close relationship with hippocampal nerve. The effect of related gene mRNA expression was studied. To explore the role and significance of neurogenesis in promoting learning and memory ability in sports training. Methods: after screening, 12 mice were selected and randomly divided into two groups: group C (control group) and group R (running wheel exercise group). All the mice in the control group were tested by Morris water maze test at the 8th week. The ability of spatial learning and memory was measured. 24 hours after the test, all mice were killed by cervical amputation method, and their hippocampal tissue was frozen and tested. RT-PCR was used to detect NCAM in hippocampus of both groups. The expression level of VEGF mRNA and IGF-1. Experimental results: 1) the results of Morris water maze test showed that the mice in wheel running group performed better in learning ability and memory than those in quiet control group. The expression of NCAM mRNA was significantly higher in the hippocampus of the mice after 8 weeks of independent wheel running than that in the quiet control group (P 0.05). The expression of SCF mRNA was significantly higher in the hippocampus of the mice after 8 weeks of independent wheel running than that in the quiet control group (P 0.05). After 8 weeks of independent wheel running, the expression of IGF-1mRNA in hippocampus of mice in the wheel running group was significantly higher than that in the quiet control group (p0.01). The expression of VEGF mRNA in the hippocampus of the mice in the wheel running group was significantly higher than that in the quiet control group after 8 weeks of independent wheel running. Conclusion: The 8 week independent wheel running can promote the spatial learning and memory ability of young mice. The expression of NCAM and SCFmRNA in the hippocampus of the mice in the wheel running group increased after 8 weeks of independent wheel running exercise. It is speculated that the hippocampal nerve in the running wheel exercise group was more than that in the quiet group. The results suggested that the 8-week self-running wheel movement might promote the development of hippocampal nerve in mice, and further suggested that the promoting effect of 8-week self-running wheel exercise on the learning and memory function of mice might be related to the development of hippocampal nerve. Eight weeks of independent wheel running could promote the expression of IGF-lmRNA in hippocampus of mice. The results suggest that 8 weeks independent running wheel exercise may promote the development of hippocampal nerve by increasing the expression of IGF-lmRNA in hippocampus of mice. Self-running wheel movement for 8 weeks could promote the expression of VEGFmRNA in hippocampus of mice. The results suggest that 8 weeks independent running wheel exercise may promote the development of hippocampal nerve by increasing the expression of VEGFmRNA in hippocampus of mice.
【学位授予单位】：华东师范大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：G804;R87

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