跑台运动预干预对睡眠剥夺大鼠行为学改变、前额叶皮质氧化应激及c-fos表达的影响

发布时间：2018-02-10 01:27

本文关键词： 跑台运动睡眠剥夺焦虑行为学习记忆氧化应激 c-fos　出处：《西安体育学院学报》2016年05期 　论文类型：期刊论文

【摘要】：目的观察4周中等强度跑台运动预干预对睡眠剥夺大鼠焦虑样行为及空间学习记忆能力、血清皮质酮含量和前额叶皮质(prefrontal cortex,PFC)氧化应激水平及c-fos表达的影响。方法将40只大鼠随机分为4组,即正常对照组、运动组、睡眠剥夺组(sleep deprivation,SD)及睡眠剥夺运动组(SD+E)。除正常对照组及睡眠剥夺组大鼠,运动组大鼠进行4周跑台运动预干预,运动结束后睡眠剥夺组及睡眠剥夺运动组大鼠睡眠剥夺建立大鼠72h SD模型(小平台水环境法),然后运用开场实验及明暗箱探索实验评估所有大鼠焦虑样行为,八臂迷宫实验测试大鼠空间学习记忆能力;行为学测试结束后检测所有大鼠血清皮质酮及前额叶皮质自由基水平,用免疫组织化学结合图像半定量方法计算前额叶皮质c-fos神经元的分布。结果 (1)与正常对照组比较,开场实验(open field test,OFT)测试中,SD组大鼠穿越格数(crossing numbers)、直立次数(rearing numbers)及中央格停留时间百分比均显著减少,粪便颗粒增多;明暗箱探索实验中(Light-dark,LD),SD组大鼠明箱停留时间及穿越次数均显著减少;八臂迷宫实验(eight arm maze tests,ERM)中SD组大鼠学习记忆能力下降,血清皮质酮水平显著增高,前额叶皮质SOD及GSH-px活性显著下降,MDA含量增加,c-fos表达显著增强。(2)与SD组大鼠比较,OFT测试中,SD+E组大鼠穿越格数、直立次数及中央格停留时间百分比均显著增加,粪便颗粒减少;LD探索实验中,SD+E组大鼠明箱停留时间显著延长,ERM实验中大鼠学习记忆能力显著改善;SD+E组大鼠血清皮质酮水平显著下降,前额叶皮质SOD活性显著上调,GSHpx活性无显著性差异,MDA含量下降,c-fos表达显著下降。结论结果显示,跑台运动预干预可以减弱睡眠剥夺对学习记忆的影响、改善睡眠剥夺大鼠焦虑样行为,可能与跑台运动改善HPA轴功能失调、增强前额叶皮质抗氧化能力及下调前额叶皮质c-fos的过度表达有关。
[Abstract]:Objective to observe the effects of 4 weeks of moderate treadmill exercise on anxiety like behavior and spatial learning and memory ability in sleep deprived rats. The effects of serum corticosterone content, prefrontal cortex prefrontal cortex-PFC-) oxidative stress level and c-fos expression in 40 rats were randomly divided into 4 groups: normal control group and exercise group. Sleep deprivation group (SD) and sleep deprivation exercise group (SD). Except normal control group and sleep deprivation group, rats in exercise group were pretreated with running platform exercise for 4 weeks. After exercise, rats in sleep deprivation group and sleep deprivation exercise group established SD model for 72 hours (small platform water environmental law), and then used open field experiment and light and dark box exploration experiment to evaluate the anxiety behavior of all rats. Eight arm labyrinth test was used to test the spatial learning and memory ability of rats, and the levels of serum corticosterone and free radical in prefrontal cortex of all rats were measured after behavioral test. The distribution of c-fos neurons in prefrontal cortex was calculated by immunohistochemistry and image semi-quantitative method. In the open field test (OFT) test, the SD rats in the SD group significantly decreased the number of crossing numbers and the percentage of residence time of the central lattice, and the fecal particles increased. The staying time and crossing times of SD group were significantly decreased, while the learning and memory ability of SD group was decreased and the serum corticosterone level was significantly increased in eight arm labyrinth experiment (eight arm labyrinth experiment). The activity of SOD and GSH-px in prefrontal cortex decreased significantly. The expression of c-fos in prefrontal cortex was significantly increased. (2) compared with SD group, the number of traverses, the number of upright times and the percentage of residence time of central lattice in SD E group were significantly increased compared with those in SD group. In the experiment of dejecta granule reduction and LD exploration, the rats in SD E group significantly prolonged the residence time of the open box in ERM experiment. The learning and memory ability of rats in SD E group was significantly improved, and the serum corticosterone level in SD E group was significantly decreased. There was no significant difference in the activity of SOD in prefrontal cortex. The expression of c-fos decreased significantly. Conclusion the results showed that the effects of sleep deprivation on learning and memory were attenuated by treadmill exercise. The improvement of anxiety like behavior in sleep deprived rats may be related to the improvement of HPA axis dysfunction in treadmill movement, the enhancement of antioxidant capacity in prefrontal cortex and the down-regulation of c-fos expression in prefrontal cortex.
【作者单位】：中北大学体育学院;太原大学体育学院;山西医科大学汾阳学院;山西体育职业学院;
【基金】：国家自然科学基金项目(30671019)
【分类号】：R87

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