间歇性低氧与规律运动对中老龄果蝇心脏泵血能力及生活质量的影响

发布时间：2018-02-15 06:38

本文关键词： 间歇性低氧规律运动心力衰竭模型心脏泵血能力生活质量　出处：《中国运动医学杂志》2017年01期 　论文类型：期刊论文

【摘要】：目的:探讨适宜的规律运动与间歇性低氧对中老龄果蝇心脏泵血功能与生活质量的影响。方法:通过扩大培养收集8 h内羽化的W1118品系处女蝇4500只,随机分为三个低氧年龄段(n=1500):青年低氧组(1~2周)、中年低氧组(3~4周)与老年低氧组(5~6周)。低氧结束后利用心力衰竭模型筛选出最佳低氧年龄段与低氧持续时间。再通过扩大培养收集8 h内羽化的W1118品系处女蝇1200只,随机分为常氧安静组(NC组)、常氧运动组(NE组)、低氧安静组(HC组)和低氧运动组(HE组)。HC与HE组果蝇采用6%O2与94%N2的混合气体,每天低氧6 h,NE与HE组果蝇每天运动2.5 h,低氧与运动干预结束的第二天进行心脏泵血能力与生活质量相关指标的检测。结果:(1)果蝇的心力衰竭率随年龄的增长而上升,中年组及老年组果蝇心力衰竭率随低氧暴露时间的延长逐渐下降。(2)NE组果蝇心脏舒张直径显著高于NC组与HC组(P0.05,P0.01),HC组显著低于NC组与HE组(P0.05,P0.01);HC组果蝇收缩直径显著低于NC组与HE组(P0.05,P0.01);NC组果蝇心脏射血分数显著低于NE组(P0.01)。(3)NE组、HC组与HE组果蝇攀爬速度均显著高于NC组(P0.05,P0.01)。(4)HC组及HE组果蝇夜晚睡眠总时间显著长于NC组(P0.05,P0.01),HC组果蝇夜晚睡眠段数显著小于NC组(P0.01)。(5)NE组果蝇活动总量显著高于其他三组(P0.05),而HC组显著低于NC组(P0.05),NE组果蝇清醒睡眠时间比以及单位活动时活动量均显著高于其他三组(P0.05),HC组果蝇单位活动时活动量显著高于NC组与HE组果蝇(P0.05),其日间活动时间显著低于NC组。(6)HC组果蝇最高寿命显著高于NC组与NE组(P0.01)。HC组果蝇平均寿命显著高于其他三组(P0.01),HE组果蝇平均寿命显著高于NC组与NE组(P0.05)。结论:规律运动与间歇性低氧均能增强中老龄果蝇的攀爬能力与日常活动能力,但与规律运动能增强中老龄果蝇心脏泵血能力不同,间歇性低氧能减小其心管直径、增强夜间睡眠质量、延长寿命,而低氧联合运动能提高中老龄果蝇的攀爬能力、延长其平均寿命和夜间睡眠时间。
[Abstract]:Objective: to investigate the effects of regular exercise and intermittent hypoxia on heart pump function and quality of life of middle-aged and aged Drosophila melanogaster. Three hypoxic age groups were randomly divided into three groups: young hypoxia group (1 ~ 2 weeks), middle age hypoxia group (n = 3 ~ 4 weeks) and aged hypoxia group (n = 5) and aged hypoxia group (n = 6). After hypoxia, heart failure model was used to screen out the best hypoxic age group and hypoxia duration. After extended culture, 1200 virgins of W1118 strain were collected within 8 hours. They were randomly divided into normoxic quiet group (NC group), normoxic exercise group (NE group), hypoxia quiet group (HC group), hypoxia exercise group (HE group) and HE group (Drosophila melanogaster) were treated with 6O2 and 94N2 mixture gas. The indexes of cardiac pumping capacity and quality of life were measured on the second day after hypoxic and exercise intervention. Results the heart failure rate of Drosophila melanogaster increased with age. Heart failure rate of Drosophila melanogaster decreased gradually with the prolongation of hypoxic exposure time. Cardiac diastolic diameter of Drosophila melanogaster in NE group was significantly higher than that in NC group and HC group (P 0.05) P0.01C group was significantly lower than that in NC group and HE group. The heart ejection fraction of drosophila in NC and HE groups was significantly lower than that in NE group (P 0.01). The climbing rate of drosophila in HC group and HE group was significantly higher than that in NC group (P 0.05) P0.01C group and HE group. The total sleep time of drosophila was significantly longer than that of NC group (P 0.05) and P 0.01 C group. The number of fruit flies in group C was significantly lower than that in group NC (P 0.01). The total activity of fruit fly in group C was significantly higher than that in the other three groups, while the ratio of awake sleep time and activity per unit activity in group HC was significantly lower than that in group C (P 0.05) and the activity per unit activity in group C was significantly higher than that in group C (P 0.05). The daily activity time was significantly higher than that in NC group and HE group, and the maximum life span of drosophila melanogaster was significantly higher than that of NC group and NE group (P 0.01). HC group was significantly higher than that of other three groups (P 0.01) and HE group (P 0.01), and the average life span of drosophila melanogaster was significantly higher than that of NC group (P 0.01) and HE group (P < 0.05), and the maximum life span of fruit fly was significantly higher than that of NC group and NE group. Conclusion: regular exercise and intermittent hypoxia can enhance the climbing ability and daily activity of the middle-aged and aged Drosophila melanogaster. But different from regular exercise, intermittent hypoxia can reduce heart tube diameter, enhance night sleep quality and prolong life span, while hypoxia combined exercise can improve climbing ability of middle-aged and aged Drosophila melanogaster. Prolong their average life span and night sleep time.
【作者单位】：湖南师范大学体适能与运动康复湖南省重点实验室;
【基金】：教育部博士点基金资助项目(20134306110009)
【分类号】：R87

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