不同负荷负重跑训练对老龄大鼠骨骼肌内MG29蛋白表达的影响

发布时间：2018-02-15 20:20

本文关键词： 衰老骨骼肌负重跑 MG蛋白　出处：《中国老年学杂志》2015年16期 　论文类型：期刊论文

【摘要】：目的探讨不同负荷负重跑训练对老龄大鼠骨骼肌内MG29蛋白表达的影响。方法 28只17月龄的健康清洁级雄性Wistar大鼠随机分为对照组、低负重组、中负重组、高负重组,低、中、高负荷组大鼠分别给予8 w最大负重30%、50%、60%的跑台训练。每周称量大鼠体重,8 w后取股直肌,常规制作冷冻切片,行苏木素-伊红(HE)染色,观察骨骼肌形态;同时检测股直肌匀浆脂褐质、钙含量水平;采用Western印迹检测MG29蛋白表达水平。结果训练8 w后,低、中、高负重组大鼠体重均明显低于训练前,以高负重组大鼠体重降低最为明显(P0.05)。对照组骨骼肌结构改变最为明显,高负重组次之,中、低负重组的排列紧密性明显高于对照组和低负重组;中、高负重组的部分肌细胞直径较大。对照组和高负重组脂褐质浓度无明显差异;低、中负重组大鼠亦无明显差异;对照组和高负重组脂褐质浓度明显高于低、中负重组(P0.05)。低、中、高负重组股直肌匀浆钙含量均高于对照组,以低负重组钙离含量升高最为明显。低、中负重组股直肌MG29蛋白水平之间无明显差异;对照组和高负重组MG29蛋白水平之间亦无明显差异;但低、中负重组明显高于对照组和高负重组(P0.05)。结论最大负重的30%、50%强度的间歇负重跑可有效提升骨骼肌中的MG29蛋白表达水平,缓解大鼠骨骼肌的衰老进程。
[Abstract]:Objective to investigate the effect of different load load on the expression of MG29 protein in skeletal muscle of aged rats. Methods 28 healthy, clean grade male Wistar rats of 17 months old were randomly divided into control group, low negative recombination, middle negative recombination, high negative recombination, low low and middle negative recombination. The rats in the high-load group were given 60% treadmill training with the maximum weight bearing at 8 weeks. The rectus femoris muscle was taken from the rat body weight 8 weeks later, then frozen sections were made routinely, and the skeletal muscle morphology was observed by hematoxylin and eosin (HEH) staining. At the same time, the lipofuscin and calcium content of rectus femoris homogenate were detected, and the expression of MG29 protein was detected by Western blotting. Results after 8 weeks of training, the body weight of low, middle and high negative recombination rats was significantly lower than that before training. The changes of skeletal muscle structure were most obvious in the control group, followed by the high negative recombination. The tightness of middle and low negative recombination was significantly higher than that of the control group and low negative recombination. There was no significant difference in the concentration of lipofuscin between the control group and the high negative recombination group, and there was no significant difference between the control group and the high negative recombinant rat, while in the control group and the high negative recombinant group, the concentration of lipofuscin was significantly higher than that in the low, medium weight bearing group (P 0.05). The content of calcium in the homogenate of high negative recombinant rectus femoris was higher than that in the control group, but there was no significant difference between the low and middle negative rectus femoris MG29 protein levels. There was no significant difference between the control group and the high negative recombinant MG29 protein level, but the middle negative recombinant protein level was significantly higher than that of the control group and the high weight bearing group (P 0.05). Conclusion the maximum load of 30% intermittent load running can effectively enhance the expression level of MG29 protein in skeletal muscle. The aging process of skeletal muscle in rats was alleviated.
【作者单位】：三峡医药高等专科学校;北京中医药大学东方医院;
【基金】：北京市自然科学基金项目(7142148) 北京市卫生系统高层次卫生技术人才项目(No.2013-3-024)
【分类号】：R87

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