培美曲塞联合放疗对肺损伤的实验研究

发布时间：2018-02-25 20:32

本文关键词： 放射性肺损伤转化生长因子β1 培美曲塞　出处：《青岛大学》2014年硕士论文　论文类型：学位论文

【摘要】：【目的]用大鼠放射性肺损伤模型模拟临床治疗,探讨培美曲塞联合照射是否会加重正常肺组织的损伤。 [方法]健康成年雄性Wister大鼠40只,随机分为正常对照组、单纯照射组、单纯药物组和药物照射同步组。照射组采用6MV-X线12Gy单次照射大鼠双肺尖,药物组采用培美曲塞20mg/kg单次腹腔注射,正常对照组予等剂量生理盐水和假照。分别于实验前1天、实验后第7、21、35、49天处死各组大鼠2只,行HE染色比较观察各组大鼠肺组织病理形态学改变、ELISA法测定血清转化生长因子β1(TGF-β1)的表达水平。 [结果]受过照射的大鼠经历了放射性肺炎到放射性肺纤维化的过程,肺组织逐渐出现炎症细胞积聚、肺间质充血水肿、肺泡壁增厚、肺组织结构消失和胶原纤维沉积；而药物组和对照组大鼠肺组织无明显异常变化。实验后第7天,单纯照射组和药物照射同步组大鼠血清TGF-β1的含量开始增高并持续到第49天,但两组之间无统计学差异(P0.05),单纯药物组和正常对照组TGF-β1的含量一直维持在正常水平,照射组分别与药物组、对照组相比较,未见统计学差异(P0.05)。 [结论]培美曲塞联合照射不会加重正常肺组织的损伤,对临床肺腺癌的同步或序贯化放疗的应用有一定的参考价值。
[Abstract]:[objective] to study whether the combined irradiation of pemetrexide can aggravate the injury of normal lung tissue by simulating the clinical treatment of rats with radiation-induced lung injury. [methods] Forty healthy adult male Wister rats were randomly divided into normal control group, simple irradiation group, single drug group and drug irradiation synchronous group. The drug group was given a single intraperitoneal injection of Pemetrexed 20mg / kg, and the control group was given the same dose of normal saline and false radiography. Two rats in each group were killed on the first day before the experiment and 3549 days after the 7th day of the experiment. The expression of TGF- 尾 1 in serum of rats with HE staining was measured by Elisa. [results] the irradiated rats experienced the process of radiation pneumonia to radiation-induced pulmonary fibrosis. Inflammatory cell accumulation, interstitial congestion and edema, alveolar wall thickening, disappearance of lung tissue structure and deposition of collagen fibers appeared in the lung tissue. On the 7th day after the experiment, the serum TGF- 尾 1 content of the rats in the single irradiation group and the drug irradiation synchronic group began to increase and continued until the 49th day after the experiment, but there was no obvious abnormal change in the lung tissue of the rats in the drug group and the control group. However, there was no statistical difference between the two groups. The content of TGF- 尾 1 in the drug group and the normal control group was maintained at the normal level, and there was no statistical difference between the irradiation group and the drug group, compared with the control group, there was no statistical difference in the content of TGF- 尾 1 between the two groups. [conclusion] combined pemetrexide irradiation can not aggravate the injury of normal lung tissue, and has certain reference value in the application of synchronous or sequential radiotherapy for clinical lung adenocarcinoma.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R730.55

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