力竭运动对大鼠血清中过氧亚硝酸阴离子生成的影响

发布时间：2018-02-26 04:21

本文关键词： 活性氧一氧化氮过氧亚硝酸阴离子力竭运动　出处：《湖南大学》2012年硕士论文　论文类型：学位论文

【摘要】：运动可以诱导自由基的生成，这一现象已被发现了三十余年，但其中的机制尚不清楚。大量研究已证实运动可导致体内超氧阴离子（O_2-）的生成，也有研究表明运动也导致骨骼肌一氧化氮合酶活性的增强，进而一氧化氮（NO）生成增加，而NO与O_2-能迅速反应形成过氧亚硝酸阴离子（ONOO~-）。ONOO~-是极强的氧化剂，推测其对运动性疲劳的产生起着至关重要的作用。本文以大鼠力竭运动为模型，诱发大鼠运动性疲劳为切入点，观察血清中各项自由基相关指标和代谢程度。以求更加深入、系统地探明是否力竭运动诱导血清ONOO~-的过量生成，为运动运动性疲劳的自由基学说提供新的理论依据。研究方法： 32只6周龄雄性SD大鼠，分成对照组（C组）和力竭运动组（E组）。E组以运动跑台构筑力竭运动模型。力竭后即刻取样检测。研究结果： 1.经力竭运动后，E组血清CK活性增加14%（P0.05），，而血清LDH没有显著性差异。 2.E组血清MDA明显增加42%（P0.05），而血清SOD活力降低了300%（P0.05）。 3.经力竭运动后，血清NO浓度升高了8%，ONOO~-更是升高18%（P0．01）。研究结论： 1.力竭运动导致血清CK活性上升，表明骨骼肌细胞损伤，大量骨骼肌细胞内的CK渗漏进入血液，产生运动性疲劳。 2.力竭运动使机体生成过量的自由基，从而使细胞脂质过氧化反应加强，生成大量MDA；同时，血清SOD活性降低，反映出SOD因清除自由基而消耗。这两个指标的变化也间接反映了力竭运动导致自由基的过量生成。 3.ONOO~-的升高其原因可能是大量的O_2-与NO反应生成，ONOO~-的生成可能是运动性疲劳的机制之一。
[Abstract]:It has been discovered for more than 30 years that exercise can induce free radical formation, but the mechanism is still unclear. A large number of studies have proved that exercise can lead to the generation of superoxide anion O _ 2-in vivo. Studies have also shown that exercise also leads to an increase in the activity of nitric oxide synthase in skeletal muscles, which in turn increases the production of nitric oxide nitrite. No and O _ 2- can react rapidly to form the peroxynitrite anion, ONOOO-, which is an extremely strong oxidant. It is inferred that it plays an important role in the generation of exercise fatigue. In this paper, using exhaustive exercise in rats as a model and inducing exercise-induced fatigue in rats as a cut-in point, we observed the related indexes of free radicals and the degree of metabolism in the serum, in order to find out whether exhaustive exercise induced the excessive production of ONOO- in serum. It provides a new theoretical basis for the free radical theory of exercise fatigue. Research methods:. Thirty-two 6-week-old male Sprague-Dawley rats were divided into control group (C group) and exhaustive exercise group (group E). Results of the study:. 1. After exhaustive exercise, the serum CK activity in E group increased by 14% (P 0.05), but there was no significant difference in serum LDH. 2. In E group, the serum MDA increased significantly (P 0.05), while the serum SOD activity decreased (P 0.05). 3. After exhaustive exercise, the concentration of no in serum increased by 8% and increased by 18% (P 0.01). The study concluded that:. 1.Exercise-induced serum CK activity increased, indicating that skeletal muscle cells were injured, a large number of CK leakage into the blood, resulting in exercise fatigue. 2. Exhausted exercise resulted in excessive production of free radicals, which enhanced cellular lipid peroxidation and produced a large number of MDAs, and decreased the activity of serum SOD. The results show that SOD is consumed by scavenging free radicals, and the changes of these two indexes also indirectly reflect the excessive production of free radicals caused by exhaustive exercise. 3. The increase of ONOO- may be due to the formation of a large number of O2s, which react with no to produce ONOOO-, which may be one of the mechanisms of exercise-induced fatigue.
【学位授予单位】：湖南大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R87

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