交变电场作用下癌细胞和正常细胞内熵产生的测量和比较

发布时间：2018-03-16 20:12

本文选题：交变电场　切入点：癌细胞　出处：《内蒙古大学》2012年博士论文　论文类型：学位论文

【摘要】：近年来,电场作用下细胞(特别是癌细胞)的生物学效应和外加电场的参数选择正在成为人们研究的热点。尽管这些研究非常的细致,但是忽略了癌细胞和正常细胞之间存在着巨大的热力学差别。细胞作为一个热力学系统,熵产生是必不可免的。癌细胞和正常细胞结构上存在着巨大的差异,通过理论计算可知在没有外场的作用下,癌细胞内部的熵产生明显高于正常细胞,两者之间的差值大约在2.7×10-16J/(K·s)～2.2×10-14J/(K·s)的范围内。熵流的方向是从癌细胞到正常细胞。但是在一定外场的作用下,正常细胞内部的熵产生高于癌细胞,可能阻止甚至改变熵流的方向。熵流伴随着信息流。这就可能提供了-种治疗癌症的方法。对交变电场作用下细胞内部的熵产生进行理论研究发现细胞内部的熵产生随电场强度的增加单调上升,电场强度在5-40V/cm范围内可能达到治疗要求。由此可见,在实验上研究细胞内部的熵产生是非常重要的。但是由于细胞体积小并且在培养基中,实验上测量细胞内部的熵产生是非常困难的。本文利用细胞在电场和环境中温度变化以及此过程中细胞的热量变化,提出一种新的测量细胞熵产生的方法,设计了测量交变电场作用下细胞内部熵产生的实验装置。为了消除不确定因素,实验时间选为300s,并且使用的温度传感器可以满足测量要求,重复实验每次都使用新的细胞,时间上尽量选为连续的两天。测量和比较了交变电场作用下两对来自人同一组织的癌细胞(MDA-MB-231人乳腺癌细胞和SMMC-7721人肝癌细胞)和正常细胞(MCF10A人乳腺上皮正常细胞和HL-7702人肝正常细胞)的熵产生。结果表明：这种方法能够非常有效的测量交变电场作用下细胞内部的熵产生。电场强度在5-40V/cm的范围内交变电场引起的癌细胞内部熵产生随着场强的增加单调上升,而正常细胞内部的熵产生是非单调上升的,电场强度在在5-30V/cm的范围内出现了一个峰值。电场强度在在5-25V/cm的范围内,电场引起的癌细胞内部熵产生和正常细胞的比值(MDA-MB-231/MCF10A和SMMC-7721/HL-7702)明显小于1。这对理论估算提供了一定的实验支持,并为进一步研究两类细胞的热力学差别提供依据。对实验数据进行了误差分析,单因素方差分析。结果表明：误差远小于实验数据,无电场作用和电场作用下细胞具有明显的统计意义。实验过程中环境温度变化大约为0.8%,在熵产生计算中完全可以忽略。我们提供了一个实验上研究细胞热力学性质非常有效的手段,并且可以进一步研究交变电场作用下正常细胞和癌细胞的差异。从实验结果来看,电场强度在5-25V/cm的范围内,交变电场作用下,正常细胞内部的熵产生要高于癌细胞内部的熵产生,并且癌细胞和正常细胞内部交变电场引起的熵产生差值达到了10-14J/(k·s),这已经达到了无外场时癌细胞和正常细胞内部熵产生的差值,这有可能阻止甚至反转癌细胞和正常细胞之间熵流和信息流方向的要求,从而达到治疗的要求,这将为治疗癌症提供了一个新的线索和依据。本工作的创新之处包括：提出了一个实验上测量活细胞内部熵产生的方法；测量和比较了交变电场作用下两对来自同一组织的癌细胞和正常细胞内部的熵产生；这将提供了一个实验上研究细胞热力学性质非常有效的手段。
[Abstract]:In recent years, the cells under the action of the electric field (especially cancer cells) selection is becoming a hot research field and the external parameters of the biological effects. Although the research is very detailed, but ignored the existence of a huge difference between the cancer cells and normal cells. Cells as a thermodynamic system, entropy generation is necessary free. Cancer cells and normal cells structure there is a huge difference, theoretical calculation shows that in the absence of external field, was significantly higher than that of normal cells to produce cancer cells within the entropy, the difference between the two is about 2.7 * 10-16J/ (K - s) ~ 2.2 * 10-14J/ (K - s) range. Entropy flow direction is to the normal cells from cancer cells. But in external field, higher than that of cancer cells to produce normal cell internal entropy, may prevent and even change the direction of entropy flow. The entropy flow along with the flow of information on this. Possible methods for the treatment of cancer. Provide a theoretical study found that with the electric field intensity increased monotonically increasing cell internal entropy generation of internal cells under alternating electric field entropy, electric field intensity may reach the treatment requirements in the range of 5-40V/cm. Thus, the research on cell internal inspection of the entropy generation is very important but because the cells in the medium and small size, measuring cell internal entropy experiment is very difficult.
The temperature in the electric field and the environment changes the cell and the heat change of the cells in this process, we proposed a new method to measure the cell entropy, the entropy measurement experimental device inside the cell under alternating electric field generated by the design. In order to eliminate the uncertain factors, experimental time is 300s, and can use the temperature sensor meet the measurement requirements, repeat the experiment every time a new cell, the time to choose for two consecutive days.
The measurement and comparison of the alternating electric field of two people from the same tissue of cancer cells (human breast cancer cell line MDA-MB-231 and SMMC-7721 in human hepatocellular carcinoma cells and normal cells (MCF10A) in normal human mammary epithelial cells and HL-7702 normal liver cells) of the entropy production. The results show that this method can measure the alternating electric field inside the cell entropy a very effective role in cancer cells. Under the internal entropy alternating electric field strength in the range of 5-40V/cm produced by electric field with the field strength increased monotonically increased, while normal cells within the entropy production is non monotonic, the electric field strength in the range of 5-30V/cm appeared a peak electric field intensity on the 5-25V/cm. Within the range of the ratio of cancer cells within the electric field produced by entropy and normal cells (MDA-MB-231/MCF10A and SMMC-7721/HL-7702) was less than 1. of the theoretical estimation provides a real The support is supported and provides a basis for further study of the thermodynamic differences between the two types of cells.
The data were analyzed by error analysis, single factor analysis of variance. The results show that the error is much smaller than the experimental data, with statistical significant cells without the electric field and the electric field. The environment temperature changes during the process of experiment is about 0.8%, completely can be ignored in the calculation of entropy.
We provide a very effective means to study the properties of cells in a thermodynamic experiment, and the differences can be further studied under alternating electric field of normal and cancer cells. From the experimental results, the electric field strength in the range of 5-25V/cm, the alternating electric field, is higher than that inside the cancer cells produce normal cell internal entropy entropy that difference reached 10-14J/ and cancer cells and normal cells within the alternating electric field induced entropy (k - s), it has already reached the difference between cancer cells and normal cells within the field entropy, which can prevent and even reverse between cancer cells and normal cells of entropy and information flow direction requirements. To achieve the treatment requirements, which will provide a new clue and basis for the treatment of cancer.
The innovation of our work includes: we propose a method for the measurement of living cells internal entropy generation; measurement and comparison of two internal entropy from the same tissue of cancer cells and normal cells under alternating electric field; this will provide an experimental study on the thermal and mechanical properties of cells are effective.

【学位授予单位】：内蒙古大学
【学位级别】：博士
【学位授予年份】：2012
【分类号】：R730.55;O441.4

【共引文献】