Flor-Essence营养补剂对运动大鼠IL-6、IL-12、肿瘤坏死因子-α和自然杀伤细胞的影响

发布时间：2018-03-19 09:28

本文选题：Flor-Essence补剂　切入点：运动大鼠　出处：《生理学报》2015年06期 　论文类型：期刊论文

【摘要】：本文旨在探讨运动和Flor-Essence营养补剂干预对大鼠免疫功能的影响。将8周龄Sprague-Dawley(SD)大鼠随机分为6组:对照组、生理盐水+训练组、低剂量Flor-Essence+训练组、低剂量Flor-Essence组、高剂量Flor-Essence+训练组和高剂量Flor-Essence组。生理盐水+训练组、低剂量Flor-Essence+训练组和高剂量Flor-Essence+训练组大鼠每日在水箱中进行游泳训练,时间为35 min/天,每周训练6天,持续4周。低剂量Flor-Essence+训练组、低剂量Flor-Essence组、高剂量FlorEssence+训练组和高剂量Flor-Essence组每日训练前灌胃2.5(低剂量)或5 mg/mL(高剂量)Flor-Essence补剂,生理盐水+训练组灌胃同体积生理盐水。在第4周最后一天对所有大鼠进行一次性力竭运动,力竭后即刻处死,取动脉血用试剂盒测定血清白细胞介素-6(IL-6)、白细胞介素-12(IL-12)及肿瘤坏死因子-α(TNF-α)含量,取脾制备单细胞悬液测定自然杀伤细胞(NK细胞)活性。结果显示,生理盐水+训练组大鼠血清IL-6含量低于对照组。与对照组相比较,低剂量和高剂量Flor-Essence组血清IL-6、IL-12和TNF-α含量降低,力竭运动时间增加。5 mg/mL Flor-Essence对于大鼠血清IL-6、TNF-α含量和力竭运动时间的改善作用要强于2.5 mg/mL Flor-Essence。与生理盐水+训练组相比,低剂量和高剂量Flor-Essence+训练组血清IL-6、TNF-α含量均降低,力竭运动时间增加;只有高剂量Flor-Essence+训练组血清IL-12含量显著降低,NK细胞活性显著增加。以上结果提示,Flor-Essence结合运动可以改善大鼠免疫功能和运动机能,且5 mg/mL浓度的作用效果要优于2.5 mg/mL。
[Abstract]:The aim of this study was to investigate the effects of exercise and Flor-Essence nutritional supplement intervention on the immune function of rats. The 8-week-old Sprague-Dawley SD rats were randomly divided into six groups: control group, saline training group, low-dose Flor-Essence training group, low-dose Flor-Essence group. The rats of the high dose Flor-Essence training group and the high dose Flor-Essence group, the saline training group, the low dose Flor-Essence training group and the high dose Flor-Essence training group had daily swimming training in the water tank for 35 min/ days, 6 days a week. After 4 weeks of training, low dose Flor-Essence training group, low dose Flor-Essence group, high dose FlorEssence training group and high dose Flor-Essence group were given intragastrically 2.5 (low dose) or 5 mg / mL (high dose floro-esence supplement) before daily training. The rats in the saline training group were given the same volume of saline at one time on the last day of week 4, and were killed immediately after exhaustion. The contents of serum interleukin-6 (IL-6), interleukin-12 (IL-12) and tumor necrosis factor- 伪 (TNF- 伪) were measured by the kit of arterial blood, and the activity of natural killer cell (NK) was determined by the single cell suspension of spleen preparation. Compared with the control group, the serum levels of IL-6, IL-12 and TNF- 伪 in the low and high dose Flor-Essence groups were lower than those in the control group. The effect of exhaustive exercise time increased by 5 mg/mL Flor-Essence on the content of IL-6 TNF- 伪 and the time of exhaustive exercise was stronger than that on 2.5 mg/mL Flor-Essence.Compared with the saline training group, the serum IL-6 TNF- 伪 content in the low dose and high dose Flor-Essence training groups was lower than that in the normal saline training group. The exhaustive exercise time was increased, and the serum IL-12 content was significantly decreased in the high dose Flor-Essence training group. The results suggested that the combination of Flor-Essence exercise and exercise could improve the immune function and motor function of rats. The effect of 5 mg/mL concentration was better than that of 2.5 mg / mL.
【作者单位】：辽宁师范大学体育学院;大连市体育科学研究所;辽宁师范大学生命科学学院;
【分类号】：R87

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