切创后细胞自噬及炎症因子的表达与损伤时间的关系

发布时间：2018-03-20 15:55

本文选题：法医病理学　切入点：皮肤切创　出处：《中国法医学杂志》2016年06期 　论文类型：期刊论文

【摘要】：目的观察小鼠皮肤切创后不同时间细胞自噬与炎症细胞因子的变化,同时探讨这些变化与损伤时间之间的关系。方法采用RT-PCR及West-blot方法检测小鼠皮肤切创组织在不同时间Beclin-1、LC-3、IL-1α、IL-1β、MCP-1的基因表达及蛋白表达。结果皮肤切创后早期IL-1α、IL-1β基因的表达及蛋白表达水平有所升高,12小时达到最高值,而后呈下降的趋势;MCP-1的表达在损伤后6h开始升高,48h达到最高值,72h有所下降,而Beclin-1、LC3基因及蛋白表达在组织损伤后6h开始升高,24h到达到最高值而后下降。结论小鼠皮肤组织切创后细胞自噬的启动及自噬的最高值明显晚于炎性细胞因子的启动及炎症因子表达的最高值,实验发现当细胞自噬水平达到最高时,炎症因子的水平则开始表现为下降的趋势。这可能是随着切创组织中炎症因子释放的增多激活了细胞的自噬,而当自噬水平达到一定的程度又抑制过度的炎症反应。自噬与炎症因子的表达随着小鼠皮肤创面损伤后不同时间呈现出一定的变化规律,这为组织损伤后损伤的时间推断提供一定的参考。
[Abstract]:Objective to observe the changes of autophagy and inflammatory cytokines at different time after skin incision in mice. Methods RT-PCR and West-blot methods were used to detect the gene expression and protein expression of RT-PCR and West-blot in mouse skin injury tissue at different time. And protein expression level increased to a peak at 12 hours. The expression of MCP-1 began to increase at 6 h after injury and reached the highest value at 48 h, and decreased at 72 h after injury. However, the expression of Beclin-1 + LC3 gene and protein began to rise to its highest value at 6 h after tissue injury, and then decreased. Conclusion the start of autophagy and the highest value of autophagy after skin injury in mice were significantly later than those of inflammatory cytokines. And the highest expression of inflammatory factors, It was found that when the level of autophagy reached its highest level, the level of inflammatory factors began to decrease. This may be that as the release of inflammatory factors in the incised tissue increased, the autophagy was activated. However, when autophagy reached a certain level and inhibited excessive inflammatory reaction, the expression of autophagy and inflammatory factors showed a certain change with different time after skin wound injury in mice. This provides a certain reference for the estimation of injury time after tissue injury.
【作者单位】：华东政法大学;
【分类号】：D919.4

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