舱室内爆炸致大鼠颅脑爆震伤模型的建立

发布时间：2018-03-22 03:20

本文选题：脑损伤　切入点：爆震伤　出处：《解放军医学杂志》2017年09期 　论文类型：期刊论文

【摘要】：目的建立一种模拟舱室内爆炸冲击波致伤的大鼠颅脑爆震伤模型。方法成年雄性SD大鼠50只,随机分为胸腹有保护3、5、8g TNT当量组,无保护5g TNT当量组和正常对照组(n=10)。将不同当量的裸药悬吊于舱室中心。除正常对照组以外,其他组大鼠麻醉后,放或不放保护胸腹装置,俯卧放置于裸药下方约31cm处,面向爆源。爆炸后,观察大鼠存活情况,于爆炸后3h、1d、3d进行血清学和病理学检查。结果从爆后组织损伤和大鼠死亡情况来看,与正常对照组相比,胸腹有保护3g TNT当量组未见明显组织损伤,全部存活;5g TNT当量组肺组织损伤较轻,脑组织水肿明显,脑表面血管增粗,有少量斑片状出血,死亡率为30%;8g TNT当量组脏器损伤严重,死亡率80%;胸腹无保护5g TNT当量组肺部损伤严重,几乎全部当场死亡。胸腹有保护5g TNT当量组致伤情况较为符合实验需求。进一步行血清学和病理检测显示:胸腹有保护5g TNT当量组大鼠脑含水量增加,血清神经元特异烯醇化酶(NSE)和S-100β蛋白含量显著升高,脑皮质和海马区神经细胞呈坏死或凋亡改变。结论大鼠在胸腹有保护的情况下,5g TNT舱室中心爆炸可建立较稳定的颅脑爆震伤动物模型。
[Abstract]:Objective to establish a model of craniocerebral explosive injury induced by explosion shock wave in cabin. Methods 50 adult male Sprague-Dawley rats were randomly divided into 3 5 g TNT equivalent group with chest and abdomen protection. Unprotected 5 g TNT equivalent group and normal control group were suspended from the center of the compartment. After anesthesia, the rats in other groups were placed on or off the protective chest and abdomen device, lying prone to the naked medicine, about 31cm under the naked medicine, except for the normal control group, the rats in the other groups were anesthetized. After explosion, the survival of rats was observed and serological and pathological examinations were performed 3 days after explosion. Results compared with the normal control group, the tissue injury and death of the rats after explosion were observed. No significant tissue damage was found in the group with 3 g TNT equivalent in chest and abdomen, but the lung tissue was slightly damaged, brain tissue edema was obvious, the blood vessels on the surface of the brain were thickened, and there was a small amount of plaque hemorrhage in the group of survival 5 g TNT equivalent. The mortality rate was 30 g / 8 g TNT equivalent, and the injury of viscera was serious in the group of 30 g / g TNT equivalent. Death rate was 80%. Lung injury was severe in unprotected 5g TNT equivalent group. Almost all of them died on the spot. The injury in the group with 5 g TNT equivalent in chest and abdomen met the need of the experiment. Further serological and pathological examination showed that the water content of brain increased in the group of 5 g TNT equivalent with the protection of chest and abdomen. The contents of serum neuron specific enolase (NSE) and S-100 尾 protein increased significantly. Conclusion under the condition of chest and abdomen protection, the central explosion of 5g TNT compartment can establish a stable animal model of craniocerebral explosive injury.
【作者单位】：海军总医院神经外科;
【基金】：国家自然科学基金(81372128) 全军后勤科研重大项目(AHJ14J001)~~
【分类号】：R-332;R826

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