大运动量和减量训练对心肌mTOR和下游信号蛋白4E-BP1的影响

发布时间：2018-03-29 18:04

本文选题：mTOR　切入点：E-BP　出处：《中国运动医学杂志》2015年03期

【摘要】：目的:探讨大运动量和减量训练对心肌哺乳动物雷帕霉素靶蛋白(m TOR)和下游信号通路的影响。方法:72只雄性SD大鼠经过1周适应性训练后,随机分为8组:3周安静对照组、3周大运动量训练组、3天对照组、3天减量训练组、6天对照组、6天减量训练组、2周对照组和2周减量训练组。3周大运动量训练采用上坡跑,在跑台上以中等强度(20 m/min,坡度10%)和大强度(26 m/min,坡度10%)交替进行,运动时间逐渐延长,由60 min增加到120 min。大运动量训练期间,每周训练6天,周1休息,共训练3周。3周大运动量训练后进行减量训练,运动强度保持不变,仍然是中等强度和大强度交替进行,但运动时间逐渐减少,由60min减少到20 min。减量的速度为阶梯式的。减量训练分为3天、6天和2周的减量训练。安静对照组在安静饲养相应天数后,各运动训练组在末次运动后12小时,麻醉取血后取左心室肌。制备心肌组织匀浆后,用Western blotting方法测定m TOR、真核生物翻译起始因子4E结合蛋白1(4E-BP1)的蛋白表达和磷酸化。结果:3周大运动量训练后进行3天减量训练,心肌m TOR(Ser2448)磷酸化显著增加。3周大运动量训练后,心肌m TOR(Ser2448)磷酸化/m TOR显著降低(P0.05)。而3天减量训练后,心肌m TOR(Ser2448)磷酸化/m TOR显著升高(P0.05)。3周大运动量训练和减量训练后,心肌4E-BP1和4E-BP1(Thr37/46)磷酸化无显著变化(P0.05)。结论:减量训练能引起心肌m TOR激活,对心脏有利。
[Abstract]:Objective: to investigate the effects of heavy exercise and reduced training on the target protein of rapamycin (rapamycin) and downstream signal pathway in myocardial mammal. Methods: 72 male Sprague-Dawley rats were trained for one week after adaptive training. They were randomly divided into 8 groups: 3 weeks quiet control group, 3 weeks heavy exercise training group, 3 days control group, 3 days reduction training group, 6 days control group, 6 days reduction training group, 2 weeks control group and 2 week reduction training group. It was alternately performed on the platform with a moderate intensity of 20 m / min (gradient 10) and a high intensity of 26 m / min. The exercise time was gradually prolonged from 60 min to 120 mins. During the heavy exercise period, the training lasted 6 days a week and rested on the 1st of the week. After 3 weeks of training, the intensity of exercise remained the same, but the exercise time was gradually reduced. 60min was reduced to 20 mins. The speed of reduction was step by step. The decrement training was divided into 3 days, 6 days and 2 weeks. The quiet control group was kept quietly for the corresponding days, and each exercise training group was 12 hours after the last exercise. After anesthesia, the left ventricular muscle was taken. After the preparation of myocardial homogenate, the protein expression and phosphorylation of m torque, eukaryotic translation initiation factor 4e binding protein 1e binding protein 4E BP1) were determined by Western blotting. After 3 weeks of training, the phosphorylation of m Torus Ser2448 (m TOR) significantly decreased P0.05N, while after 3 days of training, the phosphorylation of m Torus Ser2448) increased significantly after 3 days of training, and after 3 days of training, the phosphorylation of m torus Ser2448) increased significantly after training with large amount of exercise and decreased exercise for 3 weeks, and after 3 days of training, the phosphorylation of m torus Ser2448) increased significantly, and after 3 days of training, the phosphorylation of m torus Ser2448 increased significantly. There was no significant change in the phosphorylation of 4E-BP1 and 4E-BP1Thr37 / 46. Conclusion: deweighting training can activate myocardial m TOR, which is beneficial to the heart.
【作者单位】：燕山大学体育学院;北京体育大学运动人体科学学院;中山大学教育学院;吉林华侨外国语学院体育部;
【基金】：燕山大学博士基金项目(编号:B920) 辽宁省体育局课题(编号:LNTYJKT2014010)
【分类号】：R87

【参考文献】