单次大剂量与多次小剂量放射线构建小鼠放射性唾液腺损伤模型效果比较

发布时间：2018-04-02 13:04

  本文选题：唾液腺　切入点：放射性损伤　出处：《山东医药》2017年41期

【摘要】：目的探讨单次大剂量与多次小剂量放射线构建小鼠放射性唾液腺损伤模型的应用效果。方法选取C3H小鼠30只,随机分为空白对照组(空白组)、单次大剂量照射组(单放组)、多次小剂量照射组(多放组)各10只。空白组以0 Gy放射线照射,单放组以15 Gy放射线单次照射,多放组以每天5 Gy照射,连续5 d。分别于照射前及照射后1、5、10周测定各组唾液分泌率。照射后10周采集三组小鼠下颌下腺,分别测定腺泡细胞相对面积、细胞增殖率及毛细血管密度。结果与空白组比较,单放组、多放组唾液分泌率均下降,腺泡细胞相对面积减小,下颌下腺细胞增殖率降低,下颌下腺毛细血管密度减低,差异均有统计学意义(P均0.05)。单放组与多放组以上观察指标比较差异无统计学意义(P均0.05)。结论单次大剂量与多次小剂量放射线构建小鼠放射性唾液腺损伤模型的效果相当。
[Abstract]:Objective to investigate the effect of single dose and multiple low dose radiation on the model of radioactive salivary gland injury in mice.Methods Thirty C3H mice were randomly divided into blank control group (blank group), single large dose irradiation group (single dose group) and multiple low dose irradiation group (multiple dose group).The blank group was irradiated with 0 Gy radiation, the single radiation group with 15 Gy single dose radiation, and the multiple radiation group with 5 Gy radiation per day for 5 days.Saliva secretion rate was measured before irradiation and 10 weeks after irradiation.The submandibular glands of the three groups were collected 10 weeks after irradiation, and the relative area of acinar cells, cell proliferation rate and capillary density were measured.Results compared with the blank group, the salivary secretion rate, the relative acinar cell area, the proliferation rate and the capillary density of the submandibular gland in the single and multiple radiation groups were all decreased (P < 0.05).There was no significant difference in the above indexes between the single group and the multiple group (P < 0.05).Conclusion the effects of single large dose irradiation and multiple low dose radiation on mice with radioactive salivary gland injury are similar.
【作者单位】： 胜利油田中心医院;
【分类号】：R-332;R730.55
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本文编号：1700496