有氧运动对高血压大鼠脑动脉平滑肌细胞STOCs的影响

发布时间：2018-04-09 18:24

本文选题：有氧运动　切入点：高血压　出处：《中国运动医学杂志》2015年06期

【摘要】：目的:观察有氧运动对自发性高血压大鼠(SHR)脑动脉平滑肌细胞自发瞬时外向电流(STOCs)的影响。方法:选用雄性SHR 24只,随机分为高血压安静组(SHR-SED组)和高血压运动组(SHR-EX组),后者进行8周有氧跑台运动。另选用同龄雄性WKY大鼠12只作为正常血压对照组。8周后,取脑动脉,用酶消化法急性分离脑动脉平滑肌细胞。采用穿孔膜片钳实验记录各组大鼠脑动脉STOCs的变化。结果:(1)SHR-SED组基础收缩压显著高于WKY组,经过有氧运动训练后收缩压显著下降,但仍较WKY组高(P0.01)。(2)STOCs具有明显的电压依赖性,可被1 m M caffeine激活而被100 n M IBTX阻断;(3)在相同钳制电位下,自发性高血压可以显著增加脑动脉平滑肌细胞的STOCs的幅值,而频率无显著性改变。(4)有氧运动显著降低由高血压导致的STOCs幅值增高,而对频率无显著性影响。结论:自发性高血压大鼠脑动脉平滑肌细胞STOCs的幅值显著增大,与高血压背景下大电导钙激活钾通道(BKCa)功能的代偿性增强有关;长期有氧运动可以显著抑制此增强效应,阻止高血压背景下的BKCa通道的病理性改变。
[Abstract]:Aim: to observe the effect of aerobic exercise on spontaneous transient outward current (STOCs) of cerebral artery smooth muscle cells (SHR) in spontaneously hypertensive rats.Methods: Twenty-four male SHR rats were randomly divided into two groups: hypertension quiet group (SHR-SED group) and hypertension exercise group (SHR-EX group).In addition, 12 male WKY rats of the same age were used as the normal blood pressure control group for 8 weeks. The cerebral artery was taken and the smooth muscle cells of the cerebral artery were separated by enzyme digestion.The changes of STOCs in cerebral artery of rats were recorded by perforated patch clamp experiment.Results the basal systolic blood pressure in the SHR-SED group was significantly higher than that in the WKY group. After aerobic exercise training, the systolic blood pressure in the SHR-SED group was significantly lower than that in the WKY group, but it was still more voltage dependent than that in the WKY group. It could be activated by 1 mm caffeine and blocked by 100nM IBTX at the same clamp potential.Spontaneous hypertension significantly increased the amplitude of STOCs in cerebral artery smooth muscle cells, but the frequency did not change significantly. 4) aerobic exercise significantly decreased the increase of STOCs amplitude induced by hypertension, but had no significant effect on the frequency.Conclusion: the amplitude of STOCs in cerebral artery smooth muscle cells of spontaneously hypertensive rats is significantly increased, which is related to the compensatory enhancement of the function of large conductance calcium activated potassium channel (BK Ca) in hypertensive rats, which can be significantly inhibited by long-term aerobic exercise.To prevent pathological changes in BKCa channels in the context of hypertension.
【作者单位】：北京体育大学运动生理教研室;
【基金】：国家自然科学基金(31371201) 新世纪优秀人才支持计划(NCET-11-0850) 北京市自然科学基金(5132017) 国家级实验教学示范中心建设项目
【分类号】：R87

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