跳跃运动对大鼠胫骨骨调因子BMP2、cAMP的影响及电针干预作用

发布时间：2018-04-09 17:24

本文选题：跳跃运动　切入点：电针　出处：《苏州大学》2012年硕士论文

【摘要】：目的：本研究通过对生长期大鼠进行跳跃训练，采用电针作为直接干预手段，探讨跳跃运动及电针对生长期大鼠骨代谢的影响。从而为青少年运动员骨的正常生长发育及骨损伤的预防与治疗上提供一定的理论参考。方法：40只一月龄Wistar大鼠，随机分为空白对照组、跳跃运动组、电针预防组、电针治疗组、自然恢复组五组，每组8只。实验采用电刺激跳跃训练，针刺干预选用肾俞、足三里、阳陵泉三穴位。除空白组均进行6周电刺激跳跃训练。电针预防组每次训练结束半小时后施以电针干预。电针治疗组6周跳跃运动结束后休息一天再施以电针干预，1次/天，6天/疗程，中间休息一天，治疗3个疗程。光镜观察胫骨组织切片，双抗体夹心ABC-ELISA法测试胫骨组织BMP-2和cAMP表达。结果：（1）随着时间的增加，大鼠体重呈增加趋势，但是空白组与预防组体重在6周实验过程中差异无统计学意义；空白组与跳跃运动组大鼠体重在第2周时，前者明显高于后者，且差异具有统计学意义（P0.01）；预防组大鼠体重从第3周开始高于跳跃运动组大鼠体重，但从第4周开始预防组大鼠体重明显高于跳跃运动组，且差异有统计学意义。（2）6周跳跃运动训练后，HE结果显示跳跃运动组较空白组板层骨内胶原纤维排列明显紊乱，骨皮质密度较稀疏，骨细胞密集成团状，皮质骨内可见不规则，呈波纹状的钙化线。电针预防组较跳跃运动组大鼠胫骨板层骨内胶原纤维排列稍有紊乱，外骨膜处无可见骨溶解破坏区和肉芽组织增生，骨干鲜见局灶性粘液变性区，骨皮质密度接近正常，骨细胞分布较均匀。电针治疗组大鼠胫骨板层骨内胶原纤维排列规则有序，皮质骨内骨细胞分布均匀，骨陷窝数量少，，哈佛氏管无扩张、淤血，骨小梁排列规整有序，骨细胞分散均匀。（3）跳跃运动组大鼠胫骨组织cAMP、BMP-2的表达明显高于空白对照组与预防组，且具有显著性差异（P0.01，P0.01）；（4）治疗组大鼠胫骨组织cAMP、BMP-2的表达明显低于自然恢复组，且具有显著性差异（P0.05，P0.01）。结论：（1）根据HE染色结果提示6周的电刺激跳跃运动训练使生长期大鼠胫骨出现了骨损伤，为进一步研究跳跃运动对骨代谢的影响及骨损伤的研究提供了较好的动物模型。（2）一方面，跳跃运动可以促进cAMP和BMP-2的表达；另一方面，当骨损伤发生时也能促进cAMP和BMP-2的表达。提示跳跃运动引起的骨代谢变化与cAMP和BMP-2对骨组织的影响有关。（3）电针干预具有双向调节作用。一方面，电针干预可以降低跳跃运动对生长期骨的不良影响，对骨损伤发生具有一定的预防作用；另一方面，当骨损伤时，电针又能对骨损伤进行治疗，且具有一定的疗效。
[Abstract]:Aim: to investigate the effect of jumping exercise and electroacupuncture on bone metabolism in growing rats by using electroacupuncture as a direct intervention.It provides a theoretical reference for the normal growth and development of bone and the prevention and treatment of bone injury.Methods 40 one-month-old Wistar rats were randomly divided into five groups: blank control group, jumping exercise group, electroacupuncture prevention group, electroacupuncture treatment group and natural recovery group with 8 rats in each group.The experiment used electric stimulation jump training, acupuncture intervention selected Shenshu, Zusanli, Yanglingquan three acupoints.Except blank group, 6 weeks electric stimulation jumping training was performed.Electroacupuncture intervention was performed half an hour after each training in the electroacupuncture prevention group.In the electroacupuncture treatment group, one day of rest after 6-week jumping exercise was given, and then electroacupuncture intervention was given once a day / day for 6 days / course, and then one day for 3 courses of treatment.The expression of BMP-2 and cAMP in tibia tissue was measured by double antibody sandwich ABC-ELISA method.Results:1) with the increase of time, the body weight of the rats increased, but there was no significant difference between the blank group and the prevention group in the 6-week experiment, the weight of the blank group and the jumping group was significantly higher than that of the latter group at the second week.The body weight of the prevention group was higher than that of the jumping group from the third week, but the weight of the prevention group was significantly higher than that of the jumping exercise group from the 4th week, and the difference was statistically significant.After 6 weeks of jumping exercise training, the HE results showed that the collagenous fibers in the lamellar bone of the jumping exercise group were obviously disordered, the density of the bone cortex was sparse, the bone cells were dense and clustered, and irregular cortical calcification lines were observed in the cortex.Compared with the jumping exercise group, the collagenous fibers in the tibial lamina in the electroacupuncture prevention group were slightly disordered, no osteolysis and granulation tissue proliferation were found in the outer periosteum, focal mucinous degeneration was rarely seen in the diaphysis, and the density of the bone cortex was close to normal.The distribution of bone cells was more uniform.(3) the expression of cAMPP BMP-2 in tibial tissue in jumping exercise group was significantly higher than that in control group and prevention group, and the difference was significant (P 0.01).(4) the expression of cAMPP BMP-2 in the tibia of the treatment group was significantly lower than that in the natural recovery group, and there was significant difference between the two groups.Conclusion:1) according to the results of HE staining, 6 weeks of electric stimulation training made the tibia appear bone injury in growing rats, which provided a good animal model for further study on the effect of jumping exercise on bone metabolism and bone injury.2) on the one hand, jumping can promote the expression of cAMP and BMP-2, on the other hand, it can also promote the expression of cAMP and BMP-2 when bone injury occurs.The results suggest that the changes of bone metabolism induced by jumping exercise are related to the effects of cAMP and BMP-2 on bone tissue.3) electroacupuncture intervention has two-way regulating effect.On the one hand, electroacupuncture intervention can reduce the adverse effects of jumping exercise on the growth of bone, and has a certain preventive effect on bone injury; on the other hand, when bone injury, electroacupuncture can also treat bone injury, and has a certain effect.
【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：G804.2

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