维生素E联合红景天对低氧复合运动小鼠骨骼肌保护作用的研究

发布时间：2018-04-10 11:47

本文选题：红景天 + 维生素E　；参考：《重庆医学》2017年14期

【摘要】：目的探讨维生素E联合红景天对低氧复合运动小鼠骨骼肌细胞损伤的保护性作用及其机制。方法将40只ICR小鼠按照随机数字表法分为对照组和观察组,每组均为20只。对照组小鼠给予灌胃维生素E(40mg·kg~(-1)·d~(-1)),观察组小鼠给予灌胃维生素E(40mg·kg~(-1)·d~(-1))+红景天提取液(20g·kg~(-1)·d~(-1)),两组小鼠连续给药15d后进行为期4周的低氧(氧浓度11.3%)复合运动(5℃,15m/min,60min/d)。最后一次运动后处死小鼠检测骨骼肌血清甘肽过氧化物酶(GSH-PX)、超氧化物歧化酶(SOD)、活性氧类物质(ROS)、丙二醛(MDA);逆转录PCR(RT-PCR)法检测骨骼肌B淋巴细胞瘤-2(Bcl-2)和Bcl-2相关X蛋白(Bax)基因mRNA水平的表达,进行氧复合运动过程中(运动前、1、2、4周)监测外周血清肌酸激酶(CK)、乳酸脱氢酶(LDH)。结果运动后各时间点观察组CK与LDH活性均低于对照组(P0.05)。观察组小鼠骨骼肌组织的GSH-PX、SOD活性明显高于对照组(P0.05),而ROS、MDA低于对照组(P0.05);观察组小鼠骨骼肌组织Bax基因mRNA水平的表达与Bax/Bcl-2比值均明显低于对照组(P0.05),而Bcl-2基因mRNA水平的表达明显高于对照组(P0.05)。结论红景天联合维生素E能通过纠正低氧运动下的氧化与抗氧化失衡而抑制凋亡基因的表达。
[Abstract]:Objective to investigate the protective effect and mechanism of vitamin E combined with Rhodiola on skeletal muscle cell injury in hypoxic combined exercise mice.Methods 40 ICR mice were randomly divided into control group and observation group with 20 in each group.The expression of gene mRNA,During the oxygenation exercise (1 ~ 2 weeks before exercise), the levels of creatine kinase (CK) and lactate dehydrogenase (LDH) in peripheral blood were monitored.Results the activities of CK and LDH in the observation group were lower than those in the control group at each time point after exercise.The activity of GSH-PXO SOD in skeletal muscle of the observation group was significantly higher than that of the control group (P 0.05), while the rosid MDA was lower than that of the control group (P 0.05). The expression of Bax gene mRNA and the ratio of Bax/Bcl-2 to Bax/Bcl-2 in the skeletal muscle of the observation group were significantly lower than those in the control group (P 0.05), while the mRNA level of Bcl-2 gene in the observation group was significantly lower than that in the control group.The expression of P0.05 in the control group was significantly higher than that in the control group.Conclusion Rhodiola combined with vitamin E can inhibit the expression of apoptotic genes by correcting the imbalance of oxidation and antioxidation in hypoxic exercise.
【作者单位】：哈励逊国际和平医院骨科;武警辽宁省总队医院骨科;
【分类号】：R87

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