3周大运动量训练对大鼠骨骼肌mTOR信号通路的影响

发布时间：2018-04-10 20:22

本文选题：大运动量训练 + 骨骼肌　；参考：《中国运动医学杂志》2015年05期

【摘要】：目的:探讨3周大运动量训练过程中,大鼠骨骼肌蛋白合成代谢的变化规律和哺乳动物雷帕霉素靶蛋白(mTOR)信号通路的变化特点。方法:以7周龄雄性SD大鼠(36只)为研究对象,按体重随机分对照组3组(C1、C2、C3)和运动组(E1、E2、E3)3组。运动组进行3周大强度和中等强度相结合大运动量跑台训练,对照组不运动。每周训练结束后,随机选取对照组和运动组各1组,测试腓肠肌湿重、肌球蛋白重链(MHC)表达、mTOR及其下游信号70KD核糖体蛋白S6激酶(p70S6K)、真核起始因子4E结合蛋白1(4EBP1)的蛋白表达和磷酸化水平。结果:(1)第1周训练结束,与同期对照组(C1)相比,E1组p70S6K(Thr389)磷酸化水平和4EBP1蛋白表达显著升高(P0.01);4EBP1(Thr37/46)磷酸化水平显著升高(P0.05)。(2)第2周训练结束,与同期对照组(C2)相比,E2组mTOR(Ser2448)磷酸化水平显著降低(P0.01);p70S6K(Thr389)磷酸化水平显著降低(P0.05)。(3)第3周训练结束,与同期对照组(C3)相比,腓肠肌MHC蛋白表达显著降低,mTOR(Ser2448)和p70S6K(Thr389)磷酸化水平显著降低(P0.01);腓肠肌湿重和mTOR、p70S6K、4EBP1蛋白表达显著降低(P0.05)。结论:3周大运动量训练过程中,骨骼肌蛋白合成能力呈现出先升高后降低的变化特点。大运动量训练疲劳后对骨骼肌蛋白合成的抑制作用主要由m TOR信号通路蛋白表达降低所致。
[Abstract]:Aim: to investigate the changes of protein synthesis and metabolism in rat skeletal muscle and the characteristics of mammalian rapamycin target protein mTOR-signaling pathway during 3-week training with large amount of exercise.Methods: Thirty-six male Sprague-Dawley rats (7 weeks old) were randomly divided into three groups according to their body weight: control group (C _ (1) C _ (2) C _ (3)) and exercise group (n = 3).The exercise group received 3 weeks of high intensity and medium intensity combined with large amount of running platform training, while the control group did not exercise.The wet weight of gastrocnemius muscle was measured by random selection of control group and exercise group after weekly training.The protein expression and phosphorylation level of mTOR and its downstream signal 70KD ribosomal protein S6 kinase p70S6KN and eukaryotic initiation factor 4E binding protein 1e binding protein 1EBP1 were detected.The expression of MHC protein in gastrocnemius decreased significantly, and the phosphorylation levels of p70S6KnThr389) and the wet weight of gastrocnemius and the expression of mTORP70S6Kn4EBP1 decreased significantly.Conclusion the protein synthesis ability of skeletal muscle increased at first and then decreased during the 3 week training with large amount of exercise.The inhibition of skeletal muscle protein synthesis after heavy exercise fatigue was mainly due to the decrease of m TOR signal pathway protein expression.
【作者单位】：沈阳体育学院;北京体育大学;
【基金】：国家自然科学基金(31071034)
【分类号】：R87

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