显微CT在骨腱结合部损伤中的应用与低强度循环载荷对损伤的影响

发布时间：2018-04-13 00:25

本文选题：骨腱结合部损伤 + 循环载荷　；参考：《北京体育大学》2012年硕士论文

【摘要】：髌骨髌腱骨腱结合部损伤是常见的运动损伤,是当今体育科学界届的热点问题。在整个对髌骨髌腱骨腱结合部损伤机理及治疗手段的研究中,动物实验是其中重要的环节。本研究在Nakama循环载荷动物模型以及前阶段实验的基础上,研究阈下强度运动对骨腱结合部损伤的影响,并通过实验探讨Micro-CT技术计算纤维软骨带厚度的可行性,同时使用Micro-CT技术计算实验组和对照组的骨量和骨小梁变化。 16只成年雌性新西兰大白兔,随机筛选分组分为4周循环载荷组(n=8),8周循环载荷组(n=8),左后肢进行实验,右后肢为空白对照。实验过程中,前4周两组实验动物统一接受强度为7.17N/S的肌肉收缩刺激,一周3次,一次2小时,共24小时。实验4周后,4周循环载荷组停止实验,8周循环载荷组将强度改为4.2N/S,实验时间不变。8周后统一进行取材,将取材后组织首先进行Micro-CT进行扫描图像重建,随后进行固定、脱钙、脱水、石蜡包埋、切片和HE染色制成组织切片,使用图像采集系统进行组织学分析。结果：对比对照组,8周循环载荷组和4周循环载荷组均在髌腱出现了玻璃样变,纤维紊乱；潮线出现“涨潮”、模糊乃至消失,纤维软骨带增厚,显示损伤模型建立成功。HE染色测量纤维软骨带厚度8周循环载荷组和4周循环载荷组没有显著差异,Micro-CT计算纤维软骨带厚度结果与HE染色测量结果未见相关性,通过Micro-CT计算骨量和骨小梁结果显示实验组和对照组没有显著差异。结论：①对髌骨骨腱结合部损伤实施阈下强度载荷训练和休息的干预手段可以改变骨的微细结构,而不能改变骨腱结合部的组织变化,骨的重建和恢复能力要强于骨腱结合部；②Micro-CT测定法可以更好描述纤维软骨带厚度的假设不成立。技术由于技术特点的限制,不能准确的计算骨腱结合部纤维软骨带的面积。
[Abstract]:Patellar patellar tendon joint injury is a common sports injury and a hot issue in sports science.Animal experiment is an important part in the study of patellar patellar tendon joint injury mechanism and treatment.On the basis of the animal model of Nakama cyclic loading and the previous stage experiment, the effect of subthreshold intensity exercise on the injury of bone tendon joint was studied, and the feasibility of calculating the thickness of fibrous chondrocytes by Micro-CT technique was discussed.Micro-CT technique was used to calculate the changes of bone mass and trabeculae in the experimental group and the control group.Sixteen adult female New Zealand white rabbits were randomly divided into 4 weeks cyclic loading group and 8 week cyclic loading group. The left hind limb was tested and the right hind limb was used as blank control.In the first four weeks, the two groups received the muscle contraction stimulation with 7.17N/S intensity 3 times a week, 2 hours once for 24 hours.Four weeks after the experiment, the cyclic loading group stopped the experiment and the 8-week cyclic load group changed the strength to 4.2 N / S, the experiment time was unchanged, and the sample was collected. The tissue was reconstructed by Micro-CT first, then fixed and decalcified.Tissue sections were made by dehydration, paraffin embedding, sections and HE staining, and histologic analysis was performed by image acquisition system.Results: compared with the control group, the 8-week cyclic loading group and the 4-week cyclic load group showed glass-like changes and fibrous disorder in the patellar tendon, the tidal line appeared "high tide", blurred or even disappeared, and the fibrous cartilage band thickened.The results showed that there was no significant difference between 8-week cyclic load group and 4-week cyclic load group in the thickness of fibrous chondrocytes by HE staining. There was no correlation between the results of micro-CT and HE staining.The results of bone mass and trabeculae calculated by Micro-CT showed that there was no significant difference between the experimental group and the control group.Conclusion the intervention of subthreshold stress training and rest on patellar tendon junction injury can change the fine structure of bone, but not the tissue change of tendon junction. The ability of bone reconstruction and recovery is stronger than that of tendon junction.The hypothesis that the thickness of fibrochondrocytes can be better described by 2Micro-CT method is not true.Due to the limitation of technical characteristics, the area of fibrous cartilage band of bone tendon junction can not be calculated accurately.
【学位授予单位】：北京体育大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R873

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