定向结构软骨支架材料修复运动性关节软骨损伤

发布时间：2018-04-13 02:24

本文选题：支架 + 软骨　；参考：《中国组织工程研究》2017年02期

【摘要】：背景:软骨支架材料虽然在运动关节软骨损伤中发挥了重要的作用,但是在实际开展过程中仍受到较多的限制,导致组织工程化软骨修复达不到预期目标。目的:探讨定向结构软骨支架材料制备方法及在大白兔运动关节软骨损伤中的修复效果。方法:利用温度梯度热诱导相分离技术制备定向结构软骨支架材料,采用京尼平完成对软骨支架材料的交联,并测定支架材料的物理、力学性能。48只大白兔建立运动关节软骨损伤模型,随机将大白兔分为非定向支架组和定向支架组(n=24),分别于运动关节软骨损伤处植入非定向支架和定向支架,6,12,24周后取材,比较2种支架在大白兔运动关节损伤中的修复效果。结果与结论:1扫描电镜观察:定向支架组纵切面上可见排列相对整齐的微管样结构,且方向一致;而非定向支架组纵切面与横切面表现为多孔蜂窝状结构;2弹性模量:定向支架组支架材料弹性模量显著高于非定向支架组(P0.05);3O'Driscoll量表评分:定向支架组大白兔术后6,12,24周显微CT下O'Driscoll量表评分显著高于非定向支架组(P0.05);4CT扫描:术后24周定向支架组大白兔CT下三维重建可见缺损部位平坦、光滑,冠状面二维扫面可见新生类软骨厚度与周围正常骨十分接近;非定向组大白兔3D下显示软骨表面比较平整,冠状面2D显示修复软骨与正常软骨基本相同;5苏木精-伊红染色:定向支架组术后24周缺损部位消失,软骨细胞呈垂直方向排列;非定向支架组术后24周缺损部位存在典型软骨陷窝,与周围软骨组织存在差异;6结果提示,采用温度梯度热诱导相分离技术制备的定向微管结构软骨支架具有良好的物理、力学性能,将其植入大白兔运动关节损伤软骨中能促进损伤部位修复,获得理想的机械力学性能。
[Abstract]:Background: although cartilage scaffolds play an important role in the injury of articular cartilage, they are still limited in the process of practical development, resulting in tissue engineered cartilage repair can not achieve the desired goal.Objective: to investigate the preparation method of oriented cartilage scaffold and its effect on repairing articular cartilage injury in rabbits.Methods: the oriented cartilage scaffold materials were prepared by temperature gradient thermal induced phase separation technique. The crosslinking of cartilage scaffolds was completed by geniapine, and the physical properties of the scaffolds were measured.Mechanical properties. 48 rabbits were randomly divided into two groups: the non-directional scaffold group and the directed scaffold group.To compare the repair effect of two kinds of scaffolds in rabbit sports joint injury.Results and conclusion: the microtubules in the longitudinal plane of the directional scaffold group were observed by scanning electron microscope (SEM) with relatively neat arrangement and the direction was the same.The elastic modulus of porous honeycomb structure in non-directional stent group was significantly higher than that in non-oriented scaffold group (P 0.05) and Driscoll scale.The score of O'Driscoll scale under microCT was significantly higher than that in the non-oriented stent group (P 0.05). The defect was flat in 3D reconstruction of rabbits 24 weeks after operation in the oriented stent group.The thickness of the new cartilage was very close to that of the surrounding normal bone on two-dimensional scan of coronal plane, and the surface of the cartilage was flat in the unoriented group under 3D.Coronal 2D showed that the repair cartilage was basically the same as the normal cartilage by hematoxylin-eosin staining: in the directional stent group, the defect disappeared 24 weeks after operation, and the chondrocytes were arranged perpendicularly.In the non-directional stent group, there were typical cartilage lacunae at the defect site 24 weeks after operation. The results indicated that the oriented microtubule cartilage scaffold prepared by temperature gradient thermal induced phase separation technique had good physical properties.The mechanical properties, implanted into the articular cartilage of rabbits, can promote the repair of the injured site and obtain the ideal mechanical and mechanical properties.
【作者单位】：晋中学院体育学院;山西医科大学体育教学部;
【基金】：山西省自然科学基金计划资助项目(2014010023)~~
【分类号】：R318.08;R873

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