黄精多糖对力竭运动小鼠胸腺胸腺指数、脾脏指数、T淋巴细胞亚群、巨噬细胞吞噬功能的影响

发布时间：2018-04-15 11:19

本文选题：黄精多糖 + 力竭运动　；参考：《基因组学与应用生物学》2015年01期

【摘要】：为了探讨黄精多糖对力竭训练后小鼠胸腺指数、脾脏指数、T淋巴细胞亚群、巨噬细胞功能的影响,本研究以100昆明雄性小鼠,随机分为安静对照组、运动训练组、运动垣低剂量给药组、运动垣中剂量给药组、运动垣高剂量给药组,每组20只,在实验结束前3 d后,每组小鼠随机分为M、N两个组,M组用于胸腺指数、脾脏指数、T淋巴细胞亚群的测试,N组用于巨噬细胞功能的测定。除安静对照组外,其余各组小鼠进行为期20 d,隔天一次的无负重力竭游泳训练,三个给药组小鼠同时按50 g/kg·d、100 g/kg·d、150 g/kg·d三种剂量灌胃。测定各组小鼠胸腺指数、脾脏指数、T淋巴细胞亚群、巨噬细胞功能。结果表明运动后小鼠胸腺指数、脾脏指数、CD3+、CD4+、CD4+/CD8+、巨噬细胞吞噬率、吞噬指数明显下降。运动垣中剂量给药组、运动+高剂量给药组胸腺指数、脾脏指数、CD+3、CD+4、CD+4/CD+8、巨噬细胞吞噬率、吞噬指数显著或及显著高于运动训练组,运动垣低剂量给药组CD+3、CD+4、CD+4/CD+8显著高于运动训练组。运动运动垣高剂量给药组腺指数、脾脏指数、CD++3、CD+4、CD+4/CD8、巨噬细胞吞噬率、吞噬指数显著高于运动垣低剂量给药组。研究提示,黄精多糖可提高长期力竭训练小鼠的免疫能力,具有显著免疫增强效应,其中以高剂量黄精多糖免疫效果最佳。本研究为黄精多糖在运动免疫领域的应用提供新思路。
[Abstract]:In order to study the effects of polysaccharides of Polygonatum flavescens on thymus index, spleen index, T lymphocyte subsets and macrophage function of mice after exhaustive training, 100 Kunming male mice were randomly divided into quiet control group and exercise training group.The mice in the low dose group, the middle dose group and the high dose group, 20 rats in each group, were randomly divided into two groups for thymus index (thymus index) three days before the end of the experiment.Determination of spleen index T lymphocyte subsets in group N was used to determine the function of macrophages.In addition to the quiet control group, the rest of the mice were given exhaustive swimming training once every other day for 20 days. The mice in the three groups were given orally at 50 g/kg / 100 g/kg / d for 150 g/kg / d at the same time.Thymus index, spleen index, T lymphocyte subgroup and macrophage function were measured in each group.The results showed that the thymus index, spleen index, CD 4 / CD 4 / CD 8, phagocytosis rate and phagocytic index of macrophages decreased significantly after exercise.The thymus index, spleen index, macrophage phagocytosis rate, phagocytic index and phagocytic index were significantly higher in exercise high dose group than in exercise training group.CD _ 3 CD _ 4 CD _ 4 4/CD _ 8 in low dose group was significantly higher than that in exercise training group.The glandular index, spleen index, CD4 / CD4 / CD8, phagocytosis rate and phagocytic index of macrophages in high dose group were significantly higher than those in low dose group.The results showed that the polysaccharide could improve the immune ability of mice trained by exhaustion for a long time, and had a remarkable immune enhancement effect, among which the high dose of flavin polysaccharide had the best immune effect.This study provides a new idea for the application of Polygonatum flavescens polysaccharides in the field of exercise immunity.
【作者单位】：钦州学院体育学院;
【基金】：广西教育厅科学研究项目(201106LX531) 钦州学院科研项目(2012XJKY-15B)共同资助
【分类号】：R87

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