有氧运动对心梗大鼠心肌组织Bax、Bcl-2、p53和TGF-β1表达及心功能的影响

发布时间：2018-04-17 01:06

本文选题：有氧运动 + 心肌梗死　；参考：《北京体育大学学报》2017年02期

【摘要】：目的:探讨有氧运动对心梗大鼠心肌Bax、Bcl-2、p53和TGF-β1表达及心功能的影响。方法:选取Sprague Dawley雄性大鼠34只,体重为180~220 g,随机抽取10只为假手术组(A组),其余24只大鼠行左冠脉前降支(LAD)结扎术制作心梗(MI)模型,术后存活20只,随机分为心梗组(B组)和心梗+有氧运动组(C组),每组10只,C组进行8周跑台有氧运动。训练结束后进行心功能测定和Masson染色观察,免疫组化检测心肌Bax、Bcl-2、p53和TGF-β1表达。结果:与A组比较,B组心系数显著下降(P0.01),血流动力学指标LVSP和±dp/dtmax均显著下降(P0.01),LVEDP显著升高(P0.01),Bax、p53和TGF-β1的MOD值均显著升高,且p53和TGF-β1的MOD值升高极其显著(P0.01);与B组比较,C组心系数显著提高(P0.05),LVSP和±dp/dtmax均显著升高(P0.01),LVEDP显著降低(P0.01),心肌Bcl-2的MOD值显著升高(P0.01),Bax、p53和TGF-β1的MOD值均降低,但p53显著降低(P0.01)。结论:大鼠MI后心肌组织Bax、p53和TGF-β1的表达均显著升高,Bcl-2的表达降低,心肌细胞趋于凋亡;8周有氧运动可使MI大鼠心肌组织Bax、p53和TGF-β1的表达显著降低,Bcl-2的表达显著升高,心肌细胞凋亡抑制,TGF-β1可能通过影响Bax和Bcl-2的表达参与了心肌细胞凋亡;有氧运动可能通过抑制MI大鼠细胞凋亡,减少心肌细胞丢失,进而提高心脏功能。
[Abstract]:Aim: to investigate the effects of aerobic exercise on the expression of Baxan Bcl-2P p53 and TGF- 尾 1 and cardiac function in myocardial infarction rats.Methods: Thirty-four male Sprague Dawley rats, weighing 180 ~ 220g, were randomly selected as sham-operated group (n = 10) and left anterior descending coronary artery occlusion (LAD) ligation to make myocardial infarction (MI) model.The rats were randomly divided into two groups: myocardial infarction group (group B) and myocardial infarction aerobic exercise group (group C).After training, the cardiac function and Masson staining were observed, and the expression of Bcl-2 p53 and TGF- 尾 1 in myocardium were detected by immunohistochemistry.Results: compared with group A, the heart coefficient of group B decreased significantly (P 0.01), and the LVSP and 卤dp/dtmax of group B were significantly decreased. The MOD values of p53 and TGF- 尾 1 in group B were significantly higher than those in group A (P 0.01).Compared with group B, the cardiac coefficient of group C was significantly higher than that of group B, and the cardiac coefficient of group C was significantly higher than that of group B (P 0.05) and 卤dp/dtmax (P 0.01). The value of MOD in myocardial Bcl-2 was significantly higher than that in group B (P 0.01), but the MOD value of P0.01Baxp53 and TGF- 尾 1 was significantly lower than that of group B (P 0.01), but the value of MOD in group C was significantly lower than that in group B (P 0.05) and 卤dp/dtmax (P < 0.05), and the value of MOD in myocardial Bcl-2 was significantly higher than that in group B (P < 0.05).Conclusion: after MI, the expression of Baxo p53 and TGF- 尾 1 in myocardial tissue increased significantly and the expression of Bcl-2 decreased. Cardiomyocytes tended to apoptosis for 8 weeks aerobic exercise could significantly decrease the expression of Baxo p53 and TGF- 尾 1 in myocardial tissue of MI rats.Cardiomyocyte apoptosis inhibition TGF- 尾 1 may be involved in cardiomyocyte apoptosis by affecting the expression of Bax and Bcl-2, while aerobic exercise may decrease myocardial cell loss and improve cardiac function by inhibiting apoptosis in MI rats.
【作者单位】：丽江师范高等专科学校体育系;陕西师范大学体育学院;
【基金】：国家自然科学基金资助项目(编号:31371199)
【分类号】：R87

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