大强度耐力运动抑制骨骼肌线粒体的生物合成

发布时间：2018-04-28 11:03

本文选题：线粒体 + 蛋白激酶类　；参考：《中国组织工程研究》2015年46期

【摘要】：背景:耐力运动对骨骼肌线粒体生成影响的研究多采用中小强度,长期大强度对其有何影响还不清楚,这种影响是否涉及5’-一磷酸腺苷激活的蛋白激酶(AMPK)、沉默信息调节因子2相关酶1(SIRT1)等调节线粒体生成的信号分子也未见报道。目的:观察AMPK/SIRT1信号级联在7周不同强度耐力运动中对骨骼肌线粒体生物合成的影响。方法:42只雄性SD大鼠分为安静组、中等强度运动组和大强度运动组。运动负荷为中等强度组28 m/min,60 min/d、大强度组38 m/min,60 min/d,每周运动5 d,休息2 d,共7周。运动组动物分别在运动后即刻、6 h和24 h取材。荧光定量PCR检测骨骼肌PGC-1α、SIRT1基因表达,Western blot测定P-AMPK、SIRT1蛋白表达。结果与结论:①中等强度运动后即刻、6 h、24 h,骨骼肌PGC-1αmR NA表达分别为安静组的362%(P0.01)、657%(P0.01)、116%,P-AMPK蛋白表达分别为安静组的112%、163%(P0.05)、129%(P0.05),SIRT1蛋白和mR NA表达分别为安静组的55%(P0.05)、86%、103%和109%、155%(P0.05)、132%(P0.05)。②大强度运动后即刻、6 h、24 h,骨骼肌PGC-1αmR NA表达分别为安静组的274%(P0.01)、130%(P0.05)、68%(P0.05),P-AMPK蛋白表达分别为安静组的235%(P0.01)、166%(P0.05)、160%(P0.05),SIRT1蛋白和mR NA表达分别为安静组的199%(P0.01)、166%(P0.05)、164%(P0.05)和255%(P0.01)、292%(P0.01)、122%。结果表明:①7周中等强度耐力运动显著增加骨骼肌PGC-1α基因表达,其机制可能涉及AMPK/SIRT1信号级联。②7周大强度耐力运动造成骨骼肌PGC-1α基因表达在运动后24 h时被抑制,这一过程是以非AMPK/SIRT1信号级联依赖性方式进行的。
[Abstract]:Background: the effects of endurance exercise on mitochondria formation in skeletal muscle are mainly studied with medium and small intensity, but it is not clear how the long-term high intensity affects it. Whether this effect is involved in the regulation of mitochondrial production by AMPKN, silencing signaling factor 2 related enzyme 1, SIRT1, and so on, has not been reported. Aim: to investigate the effects of AMPK/SIRT1 signal cascade on mitochondrial biosynthesis in skeletal muscle during 7 weeks of endurance exercise. Methods 42 male Sprague-Dawley rats were divided into three groups: quiet group, moderate exercise group and high intensity exercise group. The exercise load was 28 m / min 60 min / d in the moderate intensity group, and 60 min / d in the high intensity group, 5 days a week and 2 days rest for 7 weeks. The animals in exercise group were collected at 6 h and 24 h immediately after exercise. The expression of PGC-1 伪 -sirt1 gene in skeletal muscle was detected by fluorescence quantitative PCR and the protein expression of P-AMPK- SIRT1 protein was detected by Western blot. Results and conclusion immediately after moderate exercise, the expression of PGC-1 伪 -mRNA in skeletal muscle of the quiet group was 362and P0.01, respectively. The expression of P-AMPK protein was 112512129P0.05mR in the quiet group, respectively. The expression of SIRT1 protein and mR in the rest group was 55P0.05 863% and 10915555%, respectively, and the expression of P0.05132P 0.052P 0.052P 0.052P 0.052after the intensive exercise in the quiet group, the expression of SIRT1 protein and mR protein was 55P0.05863% and 10915555%, respectively. The expression of SIRT1 protein and mR protein in the skeletal muscle of the quiet group was higher than that in the quiet group. The expression of P-AMPK protein was the same as that in the quiet group. Immediately after 6 hours and 24 hours, the expression of PGC-1 伪 -mRNA in skeletal muscle was 274T / P 0.01a ~ (130) in the quiet group, respectively. The expression of P-AMPK protein in the P0.05 / P0.05 / P0.05 / P0.05A / AMPK protein was the same as that in the quiet group respectively. The expression of P-AMPK protein in the skeletal muscle was the same as that in the quiet group (P 0.05) and in the quiet group respectively. The expression of SIRT1 protein and mR in the rest group were 199P0.01a / 166T / P0.04 / 164NA / P 0.05) and 255'/ P0.01/ 292'/ P0.01/ 1222 respectively. The results showed that moderate intensity endurance exercise at 17 weeks significantly increased the expression of PGC-1 伪 gene in skeletal muscle. The mechanism may be related to the AMPK/SIRT1 signal cascade. 27 weeks of high intensity endurance exercise resulted in the inhibition of PGC-1 伪 gene expression in skeletal muscle 24 h after exercise. This process takes place in a non-AMPK/SIRT1 signal cascade dependent manner.
【作者单位】：韩山师范学院体育学院;
【基金】：广东省自然科学基金面上项目(S2012010010767) 韩山师范学院理科团队项目(LT201203)~~
【分类号】：R87

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