慢性睡眠剥夺对大鼠骨骼肌质量和运动能力的影响

发布时间：2018-05-03 14:19

本文选题：睡眠剥夺 + 运动能力　；参考：《中国运动医学杂志》2015年03期

【摘要】：目的:通过观察慢性睡眠剥夺前后大鼠负重游泳力竭时间以及骨骼肌质量、肌糖原含量和骨骼肌蛋白质降解相关信号蛋白Akt(又称蛋白激酶B,Protein Kinase B,PKB)、叉头框蛋白O1(Forkhead box O1,Fox O1)的变化,探究慢性睡眠剥夺对运动能力的影响,并对其分子机制进行初步探讨。方法:健康成年雄性Sprague Dawley大鼠40只,经过负重游泳筛选出30只大鼠并随机分为3组:空白对照(BC)组、大平台对照(TC)组及慢性睡眠剥夺(CSD)组。CSD组大鼠采用改良多平台水环境方法(18 h/d,连续21 d)建立CSD模型。在实验开始前及CSD 21 d后分别对各组大鼠进行负重力竭游泳测试。CSD 21 d后,分离双侧腓肠肌、比目鱼肌和跖肌并称重,测定肌糖原含量;同时应用Western Blot免疫印迹法检测大鼠骨骼肌Akt、Fox O1蛋白及蛋白磷酸化的表达水平。结果:与BC和TC组相比,CSD组大鼠体重在各时间点较其他两组显著降低(P0.01);CSD组负重游泳力竭时间显著缩短(P0.01)。与BC组相比,CSD组大鼠骨骼肌质量显著降低(腓肠肌:1.539±0.138 g vs.1.821±0.159 g;跖肌:0.258±0.044 g vs.0.327±0.039 g,P0.05),但TC组骨骼肌质量显著增加(腓肠肌:2.057±0.082 g,跖肌:0.377±0.022 g,P0.05)。肌糖原含量检测结果显示各组无显著性差异。骨骼肌细胞Akt、Fox O1蛋白及磷酸化水平检测结果显示,与BC组相比,CSD组Akt、Fox O1磷酸化水平显著降低(P0.05),而TC组则显著增加(P0.05)。结论:慢性睡眠剥夺激活了骨骼肌蛋白质降解相关信号通路,引起骨骼肌质量降低,进而导致运动能力下降。
[Abstract]:Objective: to observe the effects of chronic sleep deprivation on the time of swimming exhaustion, muscle mass, muscle glycogen content and protein degradation related signal protein Akt1 (also known as protein kinase protein Kinase Kinase PKBN, O1(Forkhead box O1H Fox O1) before and after chronic sleep deprivation in rats. To explore the effect of chronic sleep deprivation on motor ability and its molecular mechanism. Methods: forty healthy adult male Sprague Dawley rats were selected by weight-bearing swimming and randomly divided into three groups: blank control group. CSD model was established by modified multi-platform water environment method (18 h / d for 21 days) in the large platform control group and chronic sleep deprivation group (CSD) group. At the beginning of the experiment and 21 days after CSD, the rats in each group were subjected to the exhaustive swimming test. After 21 days, the bilateral gastrocnemius muscle, soleus muscle and metatarsal muscle were separated and weighed, and the content of glycogen in the muscle was measured. At the same time, Western Blot Western blot was used to detect the protein and protein phosphorylation in skeletal muscle of rats. Results: compared with BC and TC groups, the body weight of SD rats in CSD group was significantly lower than that in other two groups at each time point, and the exhaustion time of weight-bearing swimming in CSD group was significantly shorter than that in other two groups. Compared with BC group, the skeletal muscle mass was significantly decreased (1. 539 卤0.138 g vs.1.821 卤0.159 g in gastrocnemius muscle and 0. 258 卤0.044 g vs.0.327 卤0.039 g P0. 05 g in metatarsal muscle, but significantly increased in TC group (gastrocnemius muscle: 2. 057 卤0.082 g, metatarsal muscle: 0. 377 卤0.022 g P0. 05). There was no significant difference in the content of muscle glycogen in each group. Compared with BC group, the level of Aktfus Fox O1 protein and phosphorylation in skeletal muscle cells decreased significantly (P 0.05), while that in TC group increased significantly (P 0.05). Conclusion: chronic sleep deprivation activates the signal pathway associated with protein degradation in skeletal muscle, resulting in a decrease in skeletal muscle mass, and then a decrease in motor ability.
【作者单位】：上海体育学院运动科学学院;第二军医大学热带医学与公共卫生学系;
【基金】：国家自然科学基金项目(81172638) 上海市人类运动能力开发与保障重点实验室(上海体育学院)项目(No.11DZ2261100) 上海市教育委员会科研创新项目(13YZ100)
【分类号】：R87

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