EPA、DHA对力竭运动小鼠骨骼肌细胞膜流动性影响的实验研究

发布时间：2018-05-13 01:16

本文选题：二十碳五烯酸（EPA） + 二十二碳六烯酸（DHA）　；参考：《辽宁师范大学》2012年硕士论文

【摘要】：1.研究目的研究ω-3系列多不饱和脂肪酸二十碳五烯酸EPA（Eicosapentaenoicacid C20:5，，n-3）、二十二碳六烯酸DHA（Docosahexaenoic C22:6,n-3）补充对力竭运动小鼠骨骼肌细胞膜流动性的影响，并探讨EPA、DHA作为力竭运动小鼠补剂的合适剂量和适当比例。 2.研究方法以雄性昆明小鼠为研究对象，随机分组，进行为期两周的EPA、DHA补充后进行一次性力竭运动，立即处死后取双侧股四头肌，提取细胞膜。测定并计算细胞膜的荧光偏振度（Polarization,P）、微粘度η、膜脂流动性(Lipid membranefluidity，LFU)表明细胞膜流动性的指标，以揭示ω-3多不饱和脂肪酸（EPA、DHA）补充对力竭运动小鼠骨骼肌细胞膜流动性的影响，并以细胞膜流动性指标为依据探讨EPA、DHA作为力竭运动小鼠补剂的合适剂量和适当比例。 3.研究结果（1）运动对照组相对于安静对照组骨骼肌细胞膜流动性显著降低。（2）一定剂量EPA、DHA补充组相对于运动对照组骨骼肌细胞膜流动性有明显改善。（3）相对于运动对照组，EPA按50mg/kgWB及以上剂量补充可明显改善骨骼肌细胞膜流动性。（4）相对于运动对照组，DHA按50mg/kgWB及以上剂量补充可明显改善骨骼肌细胞膜流动性。（5）根据均匀实验设计统计计算结果，EPA、DHA补充剂量为1:1.5时骨骼肌细胞膜流动性改善最优。 4.结论（1）EPA、DHA补充均可明显改善力竭运动小鼠骨骼肌细胞膜流动性。（2）以骨骼肌细胞膜流动性指标为依据，EPA、DHA的合适补充剂量为50mg/kgWB。（3）以骨骼肌细胞膜流动性指标为依据，EPA、DHA的合适补充比例为1:1.5。
[Abstract]:1. Research purpose To study the effects of 蠅 -3 series polyunsaturated fatty acid EPA(Eicosapentaenoicacid C20: 5N 3 and 22 carbohexaenoic acid DHA(Docosahexaenoic C 22: 6n 3 on the membrane fluidity of skeletal muscle in exhaustive exercise mice, and to explore the appropriate dosage and proportion of ePA-DHA as a complement to exhaustive exercise mice. 2. Research method Male Kunming mice were randomly divided into two groups. After two weeks of EPA-DHA supplementation, the mice were subjected to a one-off exhaustive exercise. The quadriceps femoris muscles were killed immediately and the cell membrane was extracted from the quadriceps femoris muscle. In order to reveal the effect of 蠅 -3 polyunsaturated fatty acid (EPADHA) supplementation on the membrane fluidity of skeletal muscle in exhaustive exercise mice, we measured and calculated the fluorescence polarization degree of cell membrane, microviscosity 畏, lipid fluidity and lipid fluidity (LFU), which indicated that the membrane fluidity of membrane was measured and calculated in order to reveal the effect of 蠅 -3 polyunsaturated fatty acids on the membrane fluidity of skeletal muscle in exhaustive exercise mice. The appropriate dosage and proportion of EPA-DHA as a complement to exhaustive exercise mice were discussed based on the cell membrane fluidity index. 3. Research results 1) the membrane fluidity of skeletal muscle in exercise control group was significantly lower than that in quiet control group. 2) the membrane fluidity of skeletal muscle in certain dose EPA-DHA supplementation group was significantly improved compared with that in exercise control group. The membrane fluidity of skeletal muscle was significantly improved by 50mg/kgWB supplementation at the dose of 50mg/kgWB or above compared with that of the exercise control group. (4) compared with the exercise control group, DHA supplemented with 50mg/kgWB and above dosages could significantly improve the membrane fluidity of skeletal muscle. 5) according to the statistical calculation results of uniform experimental design, the membrane fluidity of skeletal muscle was improved when the supplementation dose of EPAA DHA was 1: 1.5. 4. Conclusion DHA supplementation could significantly improve the membrane fluidity of skeletal muscle in exhaustive exercise mice. 2) according to the membrane fluidity index of skeletal muscle, the suitable supplemental dose of EPAA DHA is 50 mg / kg WB. 3) based on the fluidity index of skeletal muscle cell membrane, the suitable supplementation ratio of EPAA DHA was 1: 1.5.
【学位授予单位】：辽宁师范大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：G804.2

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