跑台训练与营养补充对小鼠骨骼肌能量代谢酶及抗疲劳能力的影响

发布时间：2018-05-19 00:11

本文选题：琥珀酸脱氢酶 + 丙酮酸激酶　；参考：《辽宁师范大学》2012年硕士论文

【摘要】：研究目的：通过选取8周龄雄性KM小鼠为研究材料，设计不同强度的跑台训练和海参小分子肽补充的组合方案，检测训练结束后小鼠骨骼肌中丙酮酸激酶（PK）、苹果酸脱氢酶(MDH)和琥珀酸脱氢酶(SDH)活性，记录各组小鼠训练完成后游泳力竭的时间，通过探讨不同运动形式和营养补充状态下能量供应系统代谢酶的变化特点和规律，揭示长期跑台训练对小鼠骨骼肌能量代谢酶的影响，为发展无氧和有氧能力提供一定的参考。最后探讨海参小分子肽对机体抗疲劳能力的影响，为运动营养品的开发提供参考。研究方法：将30只雄性KM小鼠随机分成对照组（C）、有氧运动组（T1）、无氧运动组（T2）、有氧运动营养补充组（T3）、无氧运动营养补充组（T4），每组6只。以电动动物跑台作为训练器材，首先将T1、T2、T3、T4组常规饲养3天，而后进行1周的适应性训练，速度为8m/min，10min/d，适应训练完成后，T1、T3组小鼠进行为期6周76%VO2max的有氧跑台训练，T2、T4组小鼠进行95%VO2max的无氧跑台训练，训练强度为极限强度，每周训练6天，跑台坡度为00。各组小鼠在训练结束后（6w）从眼静脉取血0.5～1.5ml，静脉血以2500rpm离心10分钟，取上清液置于4°C冰箱中保存，用于测定小鼠血清SOD、MDA含量。取静脉血完成后断颈处死，同时迅速取右腿股四头肌清洗、除去血液、滤纸拭干、称重、放入小烧杯内。用研磨器在冰浴的条件下充分研碎,使组织成为匀浆。将制备好的10%组织匀浆用低温离心机以2500rpm速度离心10min,取上清放于样品管中-70℃冷藏备用，上清液用于各种指标的测定。采用酶免法测小鼠股四头肌中MDH、SDH、PK活性。将各组数据进行统计处理，进行比较和分析。实验结果：（1）经过6周的跑台训练和营养补充后，无氧训练组小鼠股四头肌中PK活性有所增加，与对照组相比，，T2组有差异显著（P0.05），T4组差异非常显著（P0.01），T4与T2组相比差异显著（P0.05）；T3与T1相比较无显著变化。（2）经过6周的跑台训练和营养补充，有氧训练组小鼠股四头肌中MDH、SDH活性有所增加，与对照组相比，T3组有非常显著性差异（P0.01），T1组有显著性变化（P0.05），T3与T1组相比较变化显著（P0.05）；T4与T2相比较无显著性变化。（3）经过6周的跑台训练和营养补充,有氧训练组小鼠游泳力竭时间与对照组小鼠相比较变化显著（P0.01）；无氧训练组小鼠游泳力竭时间与对照组相比有所增加，T4变化显著（P0.05），T2无显著性变化，无统计学意义；T3组与T1组相比较发现有显著性变化（P0.05），T4组与T2相比较有显著性差异（P0.05）。（4）经过6周的跑台训练和营养补充，发现小鼠血清SOD、MDA发生不同程度的变化。T1、T2、T4组小鼠血清SOD含量变化非常显著（P0.01），T2组有显著性变化（P0.05）。T1、T2、T4组小鼠血清MDA含量变化非常显著（P0.01），T2组没有显著性变化。同运动形式组别之间比较有显著性差异（P0.05）。结论：（1）长期的无氧运动训练可以使小鼠骨骼肌PK活性增加，表明长期的无氧训练通过调节代谢酶的活性，提高糖酵解的能力。（2）长期有氧训练可以有效地提高小鼠骨骼肌组织的SDH，MDH活性，表明长期有氧训练可以提高身体的有氧供能的能力，增加三羧酸循环的酶的活性。（3）长期的有氧训练，有氧代谢活动显著增加;经常在无氧训练无氧代谢酶的活性也相应增加。（4）补充海参的小分子肽，可以提高SDH，MDH，PK的活性，这可能是它被吸收转换成SDH、MDH、PK的原料或者与抗疲劳能力的改善有关，具体机制不明确需要进一步研究。（5）补充海参小分子肽，可以提高小鼠的抗疲劳能力。
[Abstract]:Objective: to determine the activity of pyruvate kinase (PK), malate dehydrogenase (MDH) and succinic dehydrogenase (SDH) in skeletal muscles of mice after training, and record the swimming ability of mice after training by selecting 8 weeks male KM mice as the research materials. At the time of exhaustion, the effects of long run platform training on energy metabolism enzymes in skeletal muscles of mice were revealed by exploring the characteristics and rules of metabolic enzymes in the energy supply system under different forms of exercise and nutritional supplement. Finally, the effect of small molecular peptides on the anti fatigue ability of the body was discussed. It provides a reference for the development of sports nutritions.
Study methods: 30 male KM mice were randomly divided into control group (C), aerobic exercise group (T1), anaerobic exercise group (T2), aerobic exercise supplement group (T3), anaerobic exercise supplement group (T4), each group was used as training equipment, and the group of T1, T2, T3, T4 was raised for 3 days, and then 1 weeks of adaptive training, and then the speed of adaptive training, and the speed of 1 weeks. For 8m/min, 10min/d, after the completion of training, T1, T3 group mice were trained for 6 weeks 76%VO2max, T2, T4 group were trained with 95%VO2max without oxygen, training intensity was the ultimate strength, training 6 days a week, and 0 groups of mice at the end of training (6W) from the eye vein, 0.5 ~ 1.5ml, and venous blood for 2500rpm. For 10 minutes centrifugation, the supernatant was stored in the 4 degree C refrigerator and used to determine the serum SOD and MDA content of the mice. After the venous blood was completed, the neck was executed and the four head of the right leg was cleaned quickly. The blood was removed, the filter paper was dried and weighed in a small beaker. The tissue became a homogenate under the condition of the ice bath to make the tissue a homogenate. The tissue would be made to be a homogenate. The 10% tissue homogenate was centrifuged at the speed of 2500rpm with low temperature centrifuge for 10min, and the supernatant was stored in the sample tube at -70 C for cold storage, and the supernatant was used for the determination of various indexes. The activity of MDH, SDH and PK in the four muscles of the mouse femoris was measured by enzyme immunoassay. The data of each group were statistically processed to compare and analyze.
Experimental results:
(1) after 6 weeks of treadmill training and nutrition supplement, the activity of PK in the four head muscles of the mice of the anaerobic training group increased. Compared with the control group, there was significant difference in the T2 group (P0.05), the difference in the T4 group was very significant (P0.01), and the difference between T4 and T2 group was significant (P0.05), and there was no significant change between T3 and T1.
(2) after 6 weeks of training and nutrition supplement, the activity of MDH and SDH increased in the four head muscle of the mice of the aerobic training group. Compared with the control group, there was a significant difference in the T3 group (P0.01), the T1 group had a significant change (P0.05), and the T3 and T1 group had a significant change (P0.05), and there was no significant change in T4 and T2.
(3) after 6 weeks of treadmill training and nutrition supplement, the swimming exhaustion time of the mice in the aerobic training group was significantly higher than that of the control group (P0.01). The time of swimming exhaustion in the anaerobic training group was increased compared with the control group, and the change of T4 was significant (P0.05), and there was no significant change in T2. The T3 group and the T1 group found that there was no significant difference. Significant change (P0.05), T4 group and T2 compared with significant difference (P0.05).
(4) after 6 weeks of treadmill training and nutritional supplement, the changes of serum SOD and MDA in mice were found to vary in varying degrees.T1, T2, and the serum SOD content of the mice in group T4 was very significant (P0.01). There was a significant change in the T2 group (P0.05).T1, T2. There was no significant change in the serum content of the mice. There were significant differences (P0.05).
Conclusion: (1) long-term anaerobic exercise can increase the PK activity of skeletal muscle in mice, indicating that long-term anaerobic training can improve the ability of glycolysis by regulating the activity of metabolic enzymes.
(2) long-term aerobic training can effectively improve the SDH and MDH activity of skeletal muscle tissue in mice, indicating that long-term aerobic training can improve the capacity of aerobic energy supply of the body and increase the activity of the enzyme of the three carboxylic acid cycle.
(3) aerobic exercise has significantly increased aerobic metabolism, and the activity of anaerobic enzymes in anaerobic training has also increased correspondingly.
(4) supplementation of small molecular peptides from sea cucumbers can improve the activity of SDH, MDH and PK, which may be related to the conversion of the raw materials of SDH, MDH, PK to the improvement of anti fatigue ability.
(5) supplementation of small peptides from sea cucumber can improve the anti fatigue ability of mice.
【学位授予单位】：辽宁师范大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：G804.2

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