常规潜艇短航前后舱室内微生物气溶胶浓度、粒径及菌谱构成

发布时间：2018-05-28 00:34

本文选题：常规潜艇 + 舱室　；参考：《卫生研究》2017年03期

【摘要】：目的跟踪监测全封闭作业舱室内微生物气溶胶浓度、粒径大小及菌谱构成,评价污染程度及潜在的生物风险。方法采用安德森CA6型撞击式空气采样仪进行微生物气溶胶的富集、粒径大小的分析,采用基质辅助激光解析电离飞行时间质谱技术进行菌种鉴定。结果航行后较航行前作业舱室微生物气溶胶浓度有明显升高(t=8.507,P0.05);航行前可培养微生物鉴定分类为12个属20个种,航行后为14个属,25个种,航行后较航行前空气微生物的主要生物构成变化不大,生物多样性增加不明显。结论所调查环境的空气微生物污染水平均相对较低,但将粒径和某些条件致病菌一并作为评价因素时,仍存在健康安全隐患。
[Abstract]:Objective to monitor the concentration of microorganism aerosol, particle size and bacterial spectrum in the closed compartment, and to evaluate the pollution level and potential biological risk. Methods the microorganism aerosol was enriched and the particle size was analyzed by Anderson CA6 impinging air sampler. The bacteria were identified by matrix assisted laser desorption ionization time of flight mass spectrometry. Results the concentration of microorganism aerosol after navigation was significantly higher than that in the cabin before navigation, and the microbes could be identified as 12 genera, 20 species, 14 genera and 25 species after navigation. The main biological composition of microorganism in the air after sailing was not changed significantly, and the biodiversity was not obviously increased. Conclusion the level of air microorganism pollution in the investigated environment is relatively low, but when the particle size and some conditional pathogenic bacteria are taken as the evaluation factors, there are still hidden dangers of health and safety.
【作者单位】：青岛大学基础医学院病原生物学系;中国人民解放军第401医院;
【基金】：全军后勤科研计划面上项目(No.CJN13J007) 总装后勤部重点项目(No.BZZ14J001)
【分类号】：R847

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