多模态显影栓塞微球制备及体外显影实验

发布时间：2018-06-02 05:29

本文选题：多模态显影 + 微球　；参考：《介入放射学杂志》2017年12期

【摘要】：目的开发一种具备X线/CT/MR下多模态显影能力的明胶栓塞微球。方法采用乳化交联法制备单一携载固态纳米Fe_3O_4颗粒的明胶栓塞微球,光学显微镜观察微球形态、粒径、分散度;热重分析法检测微球内Fe_3O_4纳米颗粒载药量;X线、CT、MR检测评估多模态显影能力;利用兔心血及人血管内皮细胞行微球溶血试验及体外细胞毒性试验;观察微球弹性、溶胀特点及消毒方法。结果最优化微球合成条件为Fe_3O_4与明胶质量比为2∶1。该微球外观圆整、分散性好、成球率高(最高77.0%)、载药率高(最高73.27%)、粒径适中为(199.78±142.90)μm,具备X线/CT/MR下多模态显影能力,最优化微球CT值最高可达(1 028.0±69.5)Hu(浓度25 mg/ml),MR T_2值下降约50%(浓度4 mg/ml)。溶血试验及细胞毒性试验溶血率及吸光值与对照组相比,差异无统计学意义(P0.05)。微球在酸性溶液中表现出溶胀特性,在无水乙醇中无溶胀现象。结论以固态Fe_3O_4纳米颗粒为显影材料,明胶为聚合物材料,采用乳化交联法可成功制备载药量高、形态规则、表面光滑、不易聚集、X线/CT/MR下多模态显影能力更强、生物安全性高、消毒方便的栓塞微球。
[Abstract]:Objective to develop a gelatin embolized microsphere with multi-mode development under X-ray / CTR. Methods the gelatin embolized microspheres carrying solid Fe_3O_4 particles were prepared by emulsion crosslinking method. The morphology, particle size and dispersion of the microspheres were observed by optical microscope. Fe_3O_4 nanoparticles in microspheres were measured by thermogravimetric analysis (TGA) to evaluate their multimodal development ability, microspheres hemolysis test and in vitro cytotoxicity test were performed using rabbit heart blood and human vascular endothelial cells, and the elasticity of microspheres was observed. Swelling characteristics and disinfection methods. Results the optimum conditions for the synthesis of microspheres were as follows: the mass ratio of Fe_3O_4 to gelatin was 2: 1. The microspheres have round appearance, good dispersibility, high spheroidization rate (77.0%), high drug loading rate (73.27%), moderate diameter of 199.78 卤142.90 渭 m, multimodal development ability under X ray / CT / CT / Mr, and optimum CT value of 1 028.0 卤69.5 Hu-1 (25 mg / ml / ml) T _ 2 decreased about 50% (4 mg / ml 路ml ~ (-1). The hemolysis rate and absorptivity of hemolysis test and cytotoxicity test were not significantly different from those of the control group (P 0.05). The microspheres exhibit swelling characteristics in acid solution and no swelling in anhydrous ethanol. Conclusion solid Fe_3O_4 nanoparticles can be used as developing materials and gelatin as polymer materials, which can be successfully prepared by emulsion crosslinking method with high drug loading, regular morphology, smooth surface, better multimodal development ability and high biological safety under X ray / T / R. Sterilizing and convenient embolization of microspheres.
【作者单位】：南方医科大学南方医院(第一临床医学院)介入诊疗科;清华大学材料科学与工程先进材料教育部重点实验室;广州军区广州总医院介入治疗中心;广州军区广州总医院骨科;广州军区广州总医院放射科磁共振室;
【基金】：广东省医学科学技术研究基金(A2015176)
【分类号】：R815

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