安多霖对高功率微波辐照大鼠睾丸生精细胞Caspase-9和XIAP的影响

发布时间：2018-06-05 02:28

本文选题：高功率微波 + 安多霖　；参考：《辐射研究与辐射工艺学报》2016年06期

【摘要】：为探讨安多霖对微波辐射损伤的的防治机制,将SD雄性大鼠按质量随机分为正常对照组、辐照组和预防组(给药量分别为3、6、9 mg/kg)。预防组大鼠连续灌胃给药,对照组和辐照组给予双蒸水,20 d后辐照组和预防组大鼠行100 m W/cm~2的高功率微波(High power-microwave,HPM)全身辐照10 min。于辐照后第1、2、5天取睾丸组织制备病理标本,采用SP免疫组化检测试剂盒标定目的蛋白,Image-pro plus图像分析软件对特定染色区域进行灰度分析和测定。结果显示,辐照组大鼠睾丸细胞Caspase-9水平较对照组呈"高-低-高"的"倒抛物线"形(p0.05),给药组则随着时间呈现"先低后高"的趋势(p0.05);对于X连锁凋亡抑制蛋白(X-linked inhibitor of apoptosis protein,XIAP)而言,辐照组与对照组之间的差异不显著,而6、9 mg/kg组在辐照后第5天较辐照组显著升高(p0.05)。结果提示,Caspase-9参与了HPM致大鼠睾丸细胞凋亡的过程,安多霖对大鼠睾丸细胞的保护作用需要Caspase-9和XIAP的共同参与。
[Abstract]:In order to investigate the mechanism of Andolin preventing and treating microwave radiation injury, SD male rats were randomly divided into normal control group, irradiation group and prevention group according to their mass. Rats in the prevention group were given intragastric administration continuously, the control group and the irradiation group were given double-steamed water for 20 days. The rats in the irradiation group and the prevention group were exposed to 100m W/cm~2 high power microwave (HPM) for 10 mins. Testicular tissues were taken from testis 5 days after irradiation, and the specific staining regions were analyzed and measured by SP immunohistochemical detection kit calibrating the target protein image-pro plus image analysis software. The results showed that the Caspase-9 level of testicular cells in the irradiated group was "high-low-high" inverted parabola "in comparison with the control group, while in the treatment group it was" low first and then high "(p0.05), and for X-linked inhibitor of apoptosis protein (XIAPs) of X-linked inhibitor of apoptosis protein (X-linked inhibitor of apoptosis protein), the level of Caspase-9 in the irradiated group was higher than that in the control group. There was no significant difference between the irradiation group and the control group, but the 6on 9 mg/kg group was significantly higher than that of the irradiation group on the 5th day after irradiation. The results suggested that Caspase-9 was involved in the apoptosis of rat testicular cells induced by HPM, and the protective effect of Andolin on rat testicular cells required the joint participation of Caspase-9 and XIAP.
【作者单位】：中国疾病预防控制中心辐射防护与核安全医学所;
【分类号】：R818

【相似文献】