AR阻断剂对运动骨骼肌mTOR信号通路的影响

发布时间：2018-06-06 00:36

本文选题：AR阻断剂 + mTOR通路　；参考：《中国运动医学杂志》2015年02期

【摘要】：目的:通过阻断雄激素受体(AR)并结合运动,探讨雄激素对运动骨骼肌哺乳动物雷帕霉素靶蛋白(m TOR)信号通路的影响特点。方法:30只7周龄雄性SD大鼠,适应性训练后随机分为5组:安静对照组(C)、AR阻断剂组(F)、运动组(E)、AR阻断剂运动组(EF)、假手术组(S)。F、EF组于实验前3 d颈部皮下包埋氟他胺释缓剂,E、EF组进行为期10 d的中等强度运动,于末次运动后6 h取趾长伸肌,测定肌球蛋白重链(MHC)蛋白表达及AR、m TOR、核糖体S6蛋白激酶(p70S6K)、真核起始因子4E结合蛋白(4EBP1)基因及蛋白和磷酸化表达。结果:F组体重与C组相比显著下降(P0.01),E组无显著差异。F组MHC含量与C组相比显著下降(P0.05),E组则显著升高(P0.05);EF组与E组相比显著下降(P0.01)。F组AR m RNA、p-AR与C组相比显著下降(P0.01),E组显著升高(P0.05),EF组与E组相比显著下降(P0.01)。F组m TOR、p70S6K、4EBP1 m RNA与C组相比均显著下调(P0.05,P0.01,P0.05),E组则显著上调(P0.05),EF组与E组相比显著下降(P0.01)。F组p-m TOR、p-4EBP1、4EBP1与C组相比均显著下降(P0.01,P0.01,P0.05),E组p-m TOR、m TOR、p-p70S6K显著增加(P0.01,P0.01,P0.05),EF组与E组相比显著下降(P0.01)。结论:阻断AR可显著抑制m TOR通路活性,运动则促进m TOR通路活性增强,阻断AR后运动并不能诱导m TOR通路活性增强,表明雄激素对运动骨骼肌m TOR通路的作用主要经AR介导的非基因途径进行。
[Abstract]:Aim: to investigate the effects of androgen on the signaling pathway of rapamycin target protein (rapamycin) in the exercise skeletal muscle mammal by blocking androgen receptor ARA and combined with exercise. Methods 30 7 week-old male SD rats were selected. After adaptive training, the rats were randomly divided into five groups: the control group (n = 5), the control group (n = 10), and the control group (n = 10), the sham operation group (n = 10) and the control group (n = 10). The muscle extensor digitorum longus was taken at 6 h after the last exercise. The expression of myosin heavy chain (MHCC) protein and the expression of ARMTOR, ribosomal S6 protein kinase p70S6KN, eukaryotic initiation factor 4E binding protein 4EBP1) and protein and phosphorylation were measured. Results there was no significant difference in body weight between group F and group C. The content of MHC in group F was significantly lower than that in group C. The content of MHC in group F was significantly higher than that in group C, but it was significantly higher in group E than in group E. The level of AR m RNAp-AR in group F was significantly lower than that in group E (P 0.01) and that in group E was significantly lower than that in group C (P 0.01). The decrease of P0.05P0.05FEF group was significantly lower than that of E group P0.01U. F group decreased P0.01P0.01K4EBP1 m RNA, compared with C group, P0.05P0.01K4EBP1 m RNA was significantly down-regulated, while P0.05P0.01P0.05E group significantly up-regulated the decrease of P0.05TORP-4P1P1EB4EBP1 compared with E group. P0.01P0.01P0.01P0.05EBP1 decreased P0.01P0.01P0.05EBP1 and P0.01P0.01P0.05EBP1 significantly decreased compared with E group and P0.05P0.01P0.05EBP1 decreased P0.01P0.01K4EBP1 significantly lower than that of E group and P0.05P0.01P0.05EBP1 decreased P0.01P0.01P0.05EBP1 and P0.01P0.01P0.05EBP1 significantly decreased compared with E group. Compared with E group, the increase of P0.01P0.01P0.01P0.05F decreased significantly (P 0.01). Conclusion: blocking AR can significantly inhibit the activity of m TOR pathway, while exercise can enhance the activity of m TOR pathway, but after blocking AR, it can not induce the increase of m TOR pathway activity. The results showed that the effect of androgen on the m TOR pathway of exercise skeletal muscle was mainly mediated by AR-mediated non-gene pathway.
【作者单位】：北京体育大学;国家体育总局运动医学研究所;
【基金】：国家自然科学基金项目(31071034) 中央高校基本科研业务费专项资金资助课题(2014BS019)
【分类号】：R87

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