骨髓间充质干细胞移植治疗犬急性放射性肠病临床观察

发布时间：2018-06-06 16:19

本文选题：骨髓间充质干细胞 + 放射性肠病　；参考：《临床军医杂志》2016年12期

【摘要】：目的建立比格犬急性放射性肠病的损伤模型,观察骨髓间充质干细胞移植治疗犬急性放射性肠病的临床效果。方法将35只比格犬随机分为空白对照组(A组)、10 Gy单独照射组(B组)、12 Gy单独照射组(C组)、14 Gy单独照射组(D组)、10 Gy照射后干细胞治疗组(E组)、12 Gy照射后干细胞治疗组(F组)及14 Gy照射后干细胞治疗组(G组),每组5只。对比格犬腹部进行X线调强照射,观察单独照射、照射后干细胞治疗的比格犬的临床特点、肠镜表现、生存时间,并采用组织病理学评分对放射造成的肠损伤程度进行评估。结果比格犬肠损伤程度与放射剂量呈正相关。E、F组干细胞治疗后肠损伤组织病理学评分明显低于治疗前(P0.05);G组治疗前后比较,差异无统计学意义(P0.05)。比格犬生存时间与放射剂量呈负相关。F组生存时间明显长于C组(P0.05);E组与B组、G组与D组比较,差异无统计学意义(P0.05)。结论随着放射剂量增大,比格犬急性放射性肠病加重,生存时间缩短。骨髓间充质干细胞移植在12 Gy剂量的急性放射性肠病的治疗中发挥积极作用。
[Abstract]:Objective to establish a model of acute radiation-induced intestinal disease in Beagle dogs and to observe the clinical effect of bone marrow mesenchymal stem cell transplantation in the treatment of acute radioactive bowel disease in dogs. Methods 35 Beagle dogs were randomly divided into two groups: control group, group A, alone irradiation with 10 Gy, group B, alone irradiation, group C, group D, after 10 Gy irradiation, stem cell treatment group, group C, group E, group C, group B, group C, alone irradiation, group C, radiation alone, group C, group C Group F (n = 5) and group G (n = 5) treated with 14 Gy irradiation. X ray intensity modulated radiation was performed on the abdomen of Beagle dogs to observe the clinical characteristics, colonoscopy, survival time, and histopathological score to evaluate the degree of intestinal injury caused by radiation. Results the degree of intestinal injury in Beagle dogs was positively correlated with the dose of radiation. The histopathological score of intestinal injury in group E F was significantly lower than that in group G before and after treatment. There was no significant difference (P 0.05). The survival time of Beagle dogs was negatively correlated with the dose of radiation. The survival time of group F was significantly longer than that of group C (P 0.05) and group B (P 0.05). There was no significant difference between group G and group D (P 0.05). Conclusion with the increase of radiation dose, acute radiation-induced bowel disease in Beagle dogs is aggravated and the survival time is shortened. Bone marrow mesenchymal stem cell transplantation plays an active role in the treatment of acute radiation bowel disease with 12 Gy dose.
【作者单位】：第四军医大学西京消化病医院;沈阳军区总医院消化内科;
【基金】：军队“十二五”重点课题(BWS12J009)
【分类号】：R730.55

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