大鼠放射性脑损伤所致血脑屏障通透性改变与EBA及VEGF表达的相关性研究

发布时间：2018-06-22 09:30

本文选题：辐射损伤 + 实验性　；参考：《天津医药》2016年06期

【摘要】：目的探究脑皮质内皮屏障抗原(EBA)及血管内皮生长因子(VEGF)在放射性血脑屏障损害条件下的动态变化规律,为临床提供参考。方法使用随机数字表法将48只清洁级雄性SD大鼠分为对照组和放射性脑损伤后7、14和28 d 4组,每组12只。采用X线电子计算机断层扫描设备制备大鼠放射性脑损伤模型。大鼠按照3 m L/kg的剂量尾静脉注射3%伊文思蓝(EB),开颅并暴露脑皮质血管,放于微循环显微镜下观察EB渗出情况,并借助微循环显微成像系统评估血脑屏障通透性;使用免疫组化染色的方法来检测各组大鼠脑皮质EBA和VEGF的表达。结果与对照组相比,损伤组大鼠脑皮质微血管EB外渗量和VEGF的表达水平于伤后7、14、28 d均有不同程度升高(均P0.05),从伤后7 d至28 d逐渐降低,损伤各亚组之间差异均有统计学意义(均P0.05),二者呈正相关(r=0.898,P0.001);EBA表达水平于伤后各个时间点均下降(均P0.05),从伤后7 d至28 d逐渐升高,损伤各亚组之间差异均有统计学意义(均P0.05),与EB外渗量呈负相关(r=-0.866,P0.001)。结论大鼠放射性脑损伤后血脑屏障通透性增加与EBA表达减少、VEGF表达增加具有重要关联性。
[Abstract]:Objective to explore the dynamic changes of cerebral cortical endothelial barrier antigen (EBA) and vascular endothelial growth factor (VEGF) under the damage of the radiation blood brain barrier (BBB), and to provide reference for clinical practice. Methods 48 male SD rats were divided into control group and 4 groups of 7,14 and 28 d after radiation brain injury by random digital table method, and 12 rats in each group were treated with X ray. The rat model of radiation brain injury was prepared by a computer tomography device. Rats were injected with 3% Evans blue (EB) in the tail vein of 3 m L/kg, craniotomy and exposure of cerebral cortex vessels. The permeability of EB was observed under microcirculation microscope, and the permeability of blood brain barrier was evaluated with microcirculation microimaging system; immunohistochemical staining was used. The expression of EBA and VEGF in cerebral cortex of rats in each group was detected by color method. Results compared with the control group, the EB exudation and the expression level of VEGF in the cerebral cortex microvessels of the injured rats were elevated in different degrees (P0.05), gradually decreased from 7 d to 28 d after injury, and the differences were statistically significant (all P0.05), There was a positive correlation between the two (r=0.898, P0.001), and the expression level of EBA decreased at all time points after injury (all P0.05), and gradually increased from 7 d to 28 d after injury. The differences were statistically significant (P0.05), and negative correlation with EB exudation (r=-0.866, P0.001). Conclusion the permeability of blood brain barrier and EBA table after radiation brain injury in rats The increased expression of VEGF has an important correlation.
【作者单位】：天津医科大学基础医学院生理学与病理生理学系;天津市第三中心医院麻醉科;天津市西青医院病理科;
【基金】：科技部国家科技计划项目国际科技合作专项(2011DFA30550)
【分类号】：R730.55

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