运动训练改善高血压大鼠动脉压力反射功能

发布时间：2018-06-27 09:34

本文选题：高血压 + 运动训练　；参考：《中华高血压杂志》2015年01期

【摘要】：目的观察运动训练对高血压大鼠动脉压力反射和中枢氧化应激的影响,探讨运动训练改善压力反射功能的中枢机制。方法雄性自发性高血压大鼠(SHR)20只和正常血压Wistar大鼠24只随机分成对照组和运动训练组,运动组大鼠进行8周跑台运动。尾套法测定大鼠收缩压和心率,静脉注射苯肾上腺素(PE)和硝普钠检测动脉压力反射敏感性(BRS)。侧脑室给予超氧化物歧化酶(SOD)拟似剂Tempol和SOD抑制剂DETC,测定给药前后BRS变化。下丘脑丙二醛含量、总抗氧化能力(T-AOC)以及抗氧化酶SOD与过氧化氢酶(CAT)活性用于评估氧化应激。结果运动前,SHR收缩压明显高于Wistar大鼠(P0.01)。运动训练显著降低SHR和Wistar大鼠心率,但仅降低SHR收缩压。SHR的BRS明显小于Wistar大鼠[PE:(0.89±0.22)比(1.96±0.23)次/(min·mm Hg);硝普钠:(0.62±0.10)比(1.32±0.23)次/(min·mm Hg),均P0.01]。运动训练明显改善SHR的BRS,与SHR对照组相比,SHR运动组BRS明显提高[PE:(1.31±0.26)比(0.89±0.22)次/(min·mm Hg);硝普钠:(1.22±0.25)比(0.62±0.10)次/(min·mm Hg);均P0.05]。侧脑室给予DETC抵消运动训练改善SHR之BRS的作用(P0.05),并降低Wistar大鼠BRS(P0.05),而Tempol则改善SHR的BRS(P0.05),使SHR运动组大鼠BRS正常化。运动训练使SHR下丘脑丙二醛含量降低27%(P0.05),同时使下丘脑T-AOC、SOD和CAT活性分别增加74%、41%和62%(P0.05);运动训练使Wistar大鼠下丘脑丙二醛含量降低40%(P0.05),并使下丘脑T-AOC、SOD和CAT活性分别增加72%、30%和51%(P0.05)。结论中枢氧化应激抑制动脉压力反射,运动训练通过增强脑内抗氧化酶活性和抗氧化能力降低中枢氧化应激,从而改善高血压大鼠动脉压力反射功能。
[Abstract]:Objective to observe the effects of exercise training on arterial baroreflex and central oxidative stress in hypertensive rats and to explore the central mechanism of improving baroreflex function by exercise training. Methods Twenty male spontaneously hypertensive rats (SHR) and 24 normal blood pressure Wistar rats were randomly divided into control group and exercise training group. Systolic blood pressure and heart rate, arterial baroreflex sensitivity (BRS) and phenylephrine (PE) were measured by tail cuff method. The changes of BRS before and after administration of superoxide dismutase (SOD) were measured after administration of Tempol and DETC. The contents of malondialdehyde (MDA) in hypothalamus, total antioxidant capacity (T-AOC) and the activities of antioxidant enzyme SOD and catalase (cat) were used to evaluate oxidative stress. Results the systolic blood pressure of SHR before exercise was significantly higher than that of Wistar rats (P 0.01). Exercise training significantly decreased the heart rate of SHR and Wistar rats, but the BRS of SHR was significantly lower than that of SHR [PE: (0.89 卤0.22) vs (1.96 卤0.23) times / (min mm Hg], sodium nitroprusside: (0.62 卤0.10) vs (1.32 卤0.23) times / (min mm Hg, respectively (P0.01). Exercise training significantly improved BRS of SHR compared with control group [PE: (1.31 卤0.26) vs (0.89 卤0.22) / (min mm Hg]; sodium nitroprusside: (1.22 卤0.25) vs (0.62 卤0.10) times / (min mm Hg; P0.05]. DETC counteracting exercise training in lateral ventricle improved BRS of SHR (P0.05) and decreased BRS in Wistar rats (P0.05), while Tempol improved BRS of SHR (P0.05), which normalized BRS in SHR exercise group. Exercise training decreased the content of malondialdehyde (MDA) in hypothalamus by 27% (P0.05), and increased the activities of SOD and cat in hypothalamus by 74 1% and 62% (P0.05), respectively, and decreased the content of MDA by 40% (P0.05) in hypothalamus of Wistar rats, and increased the activities of SOD and cat in hypothalamus by 72 30% and 51% (P 0.05), respectively. Conclusion Central oxidative stress can inhibit arterial baroreflex and exercise training can improve arterial baroreflex function by increasing the activity of antioxidant enzymes and reducing central oxidative stress in hypertensive rats.
【作者单位】：福鼎市医院神经内科;福建医科大学医学技术与工程学院康复治疗学系;
【基金】：国家自然科学基金项目(81372111) 福建医科大学教授基金(JS09001)
【分类号】：R87

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