低氧训练时心肌组织细胞低氧诱导因子1α与凋亡相关蛋白的表达

发布时间：2018-07-10 06:21

本文选题：缺氧 + 缺氧诱导因子　；参考：《中国组织工程研究》2015年51期

【摘要】：背景:运动对心肌细胞凋亡的影响有了比较多的研究,但低氧训练对心肌细胞凋亡的研究不多见。低氧诱导因子1α是否控制了Bcl-2家族的表达而影响凋亡发生,尚未明确。目的:分析低氧训练时心肌组织低氧诱导因子1α蛋白表达情况与凋亡蛋白的相互关系。方法:将健康雄性SD大鼠60只,随机分为6组:(1)对照组常温常氧下喂养,不运动。(2)低氧组在低氧环境下喂养,不运动。(3)运动组常温常氧下运动。(4)低住高练组常氧下居住,低氧、常氧训练交替进行。(5)高住低练组低氧下居住,常氧下训练。(6)高住高练低练组低氧下居住,低氧、常氧训练交替进行。运动组大鼠负荷跑台训练8周,训练每周6 d,运动速度和运动时间连续递增,运动速度从开始的10 m/min、持续时间为15 min递增至第8周的25 m/min、持续时间为50 min。低氧程度由开始从海拔1 500 m增加到第8周达到海拔3 600 m。训练结束后,运用苏木精-伊红染色、TUNEL法和蛋白免疫组织化学方法检测大鼠心肌组织细胞低氧诱导因子1α、Bcl-2、Bax的表达变化。结果与结论:(1)常氧时低氧诱导因子1α几乎不表达,低氧可增加低氧诱导因子1α的蛋白表达,低氧复合运动,表达更多。(2)低氧组和低氧运动组大鼠心肌组织Bax表达变化不是很明显,但Bcl-2表达就远远低于常氧不运动组。(3)低氧组、运动组与低氧运动组心肌组织细胞凋亡较明显,但三者相互之间比较差别较小,说明低氧结合运动不会使细胞凋亡更加严重。结果表明各实验组Bcl-2表达显著低于对照组,Bax表达变化不明显。但低氧可增加低氧诱导因子1α的蛋白表达,低氧复合运动,表达更多。说明低氧诱导因子1α有可能调节Bcl-2、Bax的表达,从而控制细胞凋亡的发生与否。
[Abstract]:Background: the effect of exercise on cardiomyocyte apoptosis has been studied, but hypoxia training on cardiomyocyte apoptosis is rare. It is not clear whether hypoxia inducible factor 1 伪 controls the expression of Bcl-2 family and affects apoptosis. Aim: to investigate the relationship between the expression of hypoxia inducible factor 1 伪 protein and apoptotic protein during hypoxia training. Methods: sixty male Sprague-Dawley rats were randomly divided into 6 groups: (1) the control group was fed under normoxic and normoxic conditions, (2) the hypoxic group was fed under hypoxic environment, (3) the exercise group was trained under normal temperature and normoxic oxygen, (4) the low living high training group lived under normal oxygen and hypoxia. (5) living under hypoxia and training under normal oxygen in high living low training group. (6) living under hypoxia, hypoxia and normoxic training in high living high training and low training group. In the exercise group, the exercise speed and exercise time increased continuously from 10 m / min at the beginning to 25 m / min at the 8th week, and the duration increased from 15 min to 25 m / min at the 8th week. The degree of hypoxia increased from 1 500 m to 3 600 m in the 8th week. After training, the expression of hypoxia inducible factor-1 伪 -Bcl-2Bcl-2 Bax in rat cardiomyocytes was detected by hematoxylin-eosin staining Tunel method and protein immunohistochemical method. Results and conclusion: (1) hypoxic inducible factor-1 伪 (HIF-1 伪) was almost not expressed in normoxic rats. Hypoxia increased the protein expression of hypoxia inducible factor-1 伪 (HIF-1 伪). (2) the expression of Bax in myocardium of hypoxic group and hypoxic exercise group was not obvious, but the expression of Bcl-2 was much lower than that of normoxic exercise group. (3) the apoptosis of cardiomyocytes in hypoxic group, exercise group and hypoxic exercise group was more obvious than that in hypoxic group, but the expression of Bcl-2 was much lower than that in normoxic exercise group. However, the differences among the three groups were small, indicating that hypoxic binding exercise did not make apoptosis more serious. The results showed that the expression of Bcl-2 in each experimental group was significantly lower than that in control group. However, hypoxia increased the protein expression of hypoxia inducible factor 1 伪, and increased the expression of hypoxia complex exercise. It is suggested that hypoxia inducible factor 1 伪 may regulate the expression of Bcl-2P Bax and control the occurrence of apoptosis.
【作者单位】：广西民族大学相思湖学院;
【基金】：广西教育厅科研项目(KY2015YB499)资助~~
【分类号】：R87

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