补充活性肽对骨骼肌Calpain基因表达的影响

发布时间：2018-07-11 17:50

本文选题：活性肽 + Calpain　；参考：《北京体育大学》2012年硕士论文

【摘要】：1研究目的本实验通过观察补充活性肽对Calpain-1.Calpain-2基因表达的影响来探讨活性肽在预防骨骼肌蛋白降解中所发挥的作用,另外还期望在亚细胞水平对骨骼肌微损伤的生理调节机制进行一定程度的阐释 2研究方法8周龄雄性SD大鼠随机分为安静对照组,运动后即刻、24h、48h对照组,运动后即刻、24h、48h+海洋肽实验组,运动后即刻、24h、48h+大豆蛋白阳性对照组,共10组,每组10只,进行6周跑台训练。采用放射免疫法测定血清T.IGF-1含量；采用Real Time-PCR法测定骨骼肌Calpain-1.Calpain-2mRNA含量。 3研究结果3.1运动后,海洋肽运动组血清IGF-1与单纯运动组、大豆蛋白运动组无显著性差异；随时间变化,运动后即刻与运动前差异显著(P0.05)。3.2运动后24h,海洋肽运动组血清睾酮与单纯运动组差异显著(P0.05)；随时间变化无显著性差异。3.3海洋肽运动组运动后即刻股中肌CANP-1基因表达变化与单纯运动组、大豆蛋白运动组差异显著(P0.05)；随时间变化,差异非常显著(P0.05)。运动后24h,股中肌CANP-2基因表达变化与大豆蛋白运动组差异显著(P0.05)；随时间变化,无显著性差异。3.4海洋肽运动组运动后24h,腓肠肌CANP-1基因表达变化与单纯运动组差异显著(P0.05)；运动后48h,与大豆蛋白运动组差异显著(P0.01)；随时间变化,无显著性差异。运动后即刻、运动后48h,腓肠肌CANP-2基因表达变化与大豆蛋白运动组差异显著(P0.01);随时间变化,差异非常显著(P0.01)。 4研究结论4.1海洋肽与大豆蛋白均能够缩短运动后血清IGF-1、血清T的恢复时间；海洋肽的效果好于大豆蛋白,促进睾酮恢复的速率更快。4.2运动后,骨骼肌CANP-1、CANP-2基因表达呈现不同变化；CANP-1基因表达随时间延长持续下调,而CANP-2基因表达上调。4.3海洋肽在运动过程中对骨骼肌有一定的保护作用,而大豆蛋白主要在运动后48h的炎症反应阶段发挥保护作用。4.4运动后股中肌CANP-1基因表达持续下调,而腓肠肌CANP-1.CANP-2基因表达呈现为先增高后降低,这与肌纤维类型有关。
[Abstract]:Objective to investigate the role of active peptides in the prevention of skeletal muscle protein degradation by observing the effect of supplementation of active peptides on the expression of Calpain-1.Calpain-2 gene. In addition, it is expected to explain to a certain extent the physiological regulation mechanism of skeletal muscle microdamage at the subcellular level. Methods the 8-week-old male SD rats were randomly divided into quiet control group and control group for 24 h and 48 h immediately after exercise. Immediately after exercise, 10 rats in 10 groups were treated with marine peptide for 24 hours and 48 hours after exercise, and the control group with soybean protein positive for 24 hours and 48 hours after exercise. 10 rats in each group were trained by running platform for 6 weeks. The levels of serum T.IGF-1 and Calpain-1.Calpain-2 mRNA in skeletal muscle were measured by radioimmunoassay and Real Time-PCR respectively. 3 the results showed that there was no significant difference in serum IGF-1 between marine peptide exercise group and soybean protein exercise group after 3.1 exercise. There were significant differences in serum testosterone between exercise group and exercise group (P0.05) immediately after exercise and 24 hours after exercise (P0.05). There was no significant difference with time. 3.3 the changes of CANP-1 gene expression in middle femoral muscle immediately after exercise in marine peptide exercise group were significantly different from those in simple exercise group and soybean protein exercise group (P0.05), and the difference was very significant with time (P0.05). The changes of CANP-2 gene expression in middle femoris muscle were significantly different from those in soybean protein exercise group at 24 h after exercise (P0.05), but there was no significant difference between exercise group and exercise group at 24 h after exercise (P0.05), and there was no significant difference in CANP-1 gene expression in gastrocnemius muscle between exercise group and exercise group 24 hours after exercise (P0.05). There was a significant difference between the exercise group and the soybean protein group 48 hours after exercise (P0.01), but there was no significant difference with time. The changes of CANP-2 gene expression in gastrocnemius muscle immediately after exercise and 48 hours after exercise were significantly different from those in soybean protein exercise group (P0.01). The difference was very significant (P0.01). 4 conclusion 4.1 Marine peptide and soybean protein can shorten the recovery time of serum IGF-1 and serum T after exercise, the effect of marine peptide is better than that of soybean protein, and the recovery rate of testosterone is faster after exercise .4.2. The expression of CANP-1 and CANP-2 in skeletal muscle showed different changes. CANP-1 gene expression continued to be down-regulated over time, while CANP-2 gene expression up-regulated .4.3 marine peptide had protective effect on skeletal muscle during exercise. The expression of CANP-1 gene in femur muscle decreased continuously after exercise 48 h after exercise, while CANP-1. CANP-2 gene expression in gastrocnemius muscle increased first and then decreased, which was related to the type of muscle fiber.
【学位授予单位】：北京体育大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：G804.2

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