蛋膜蛋白补充对大鼠一次性力竭运动损伤的预防作用

发布时间：2018-07-14 08:01

【摘要】：目的探讨蛋膜蛋白提取物(Esm)补充对一次性下坡跑运动损伤的影响及可能机制。方法雄性SD大鼠14只,随机分为普通鼠料对照组(CON)和Esm实验组。Esm实验组每天灌胃补充Esm 200 mg/(kg bw);对照组补充等量的酪蛋白,2w后进行一次性下坡跑运动。运动后空腹12h取材,检测血清乳酸、尿素、丙二醛等指标,肌肉组织检测葡萄糖醛酸酶活性和肿瘤坏死因子α(TNF-α)蛋白表达。结果一次力竭性运动后,Esm组大鼠血清乳酸、尿素和丙二醛(MDA)浓度均显著低于对照组(P0.05)。Esm组大鼠血清肌酸激酶(CK)和乳酸脱氢酶(LDH)尽管低于对照组,但没有显著性变化(P0.05)。Esm补充显著降低了骨骼肌组织中葡萄糖醛酸酶活性(P0.05)。蛋白印迹结果显示2w的Esm补充显著降低了运动后骨骼肌组织中TNF-α蛋白的表达(P0.05);且比目鱼肌组织中TNF-α表达显著高于股四头肌组织中的表达(P0.05)。结论 Esm对运动损伤有一定保护作用,其机制可能促进机体自由基清除和调节骨骼肌TNF-α的表达有关,从而加快运动后疲劳的消除。
[Abstract]:Objective to investigate the effect and possible mechanism of egg membrane protein extract (Esm) supplementation on one-time downhill running injury. Methods 14 male Sprague-Dawley rats were randomly divided into normal diet control group (Con) and Esm experimental group. ESM experimental group was given the same amount of casein for 2 weeks after oral administration of ESM 200 mg/ (kg bw);. Serum lactate, urea, malondialdehyde (MDA), glucuronidase activity and tumor necrosis factor- 伪 (TNF- 伪) protein expression were detected in muscle tissue. Results the concentrations of serum lactate, urea and malondialdehyde (MDA) in the ESM group were significantly lower than those in the control group (P0.05). The levels of creatine kinase (CK) and lactate dehydrogenase (LDH) in the ESM group were lower than those in the control group. But there was no significant change (P0.05). ESM supplementation significantly decreased the activity of glucuronidase in skeletal muscle (P0.05). Western blot analysis showed that the expression of TNF- 伪 protein in skeletal muscle was significantly decreased after 2 weeks of ESM supplementation (P0.05), and the expression of TNF- 伪 in soleus muscle tissue was significantly higher than that in quadriceps femoris tissue (P0.05). Conclusion ESM has protective effect on exercise injury, and its mechanism may be related to free radical scavenging and regulating the expression of TNF- 伪 in skeletal muscle, thus accelerating the elimination of fatigue after exercise.
【作者单位】：上海体育学院运动科学学院;上海市农业科学院;
【基金】：“运动健身科技”省部共建教育部重点实验室(上海体育学院),国家自然科学基金(No.31371197)
【分类号】：R873

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