MR扩散张量成像活体评估兔坐骨神经放射性损伤

发布时间：2018-08-17 18:18

【摘要】：目的探讨DTI评估兔坐骨神经放射性损伤的可行性和准确性,分析各弥散参数(FA、ADC、λ⊥、λ∥)与病理及肢体功能的关系。方法选取21只新西兰大白兔,随机选取一侧后肢行单次立体定向辐照制作坐骨神经放射性损伤模型。对实验兔于辐照前、辐照后1天、1、2、3、4个月行DTI及T2WI、SPIR扫描,同时评价肢体功能变化,检查完毕后于各时间点随机处死2只兔行电镜检查。结果辐照前,辐照侧与对照侧各DTI参数差异均无统计学意义(P均0.05)。辐照后1天,辐照侧FA值与对照侧差异无统计学意义(P=0.075);辐照后1、2、3、4个月辐照侧FA值均低于对照侧(P均0.05)。辐照后1天、1、2、3、4个月,辐照侧λ⊥及ADC值均高于对照侧(P均0.05)。FA值与肢体功能呈正相关(r=0.833,P=0.039),λ⊥值与肢体功能呈负相关(r=-0.833,P=0.039),ADC、λ∥值与肢体功能无相关性(r=-0.586、-0.463,P均0.05)。结论 FA及λ⊥随时间变化的趋势与肢体功能及病理改变相一致,可作为评价兔周围神经放射性损伤的可靠方法。
[Abstract]:Objective to investigate the feasibility and accuracy of DTI in assessing the radiation-induced injury of sciatic nerve in rabbits, and to analyze the relationship between the diffusion parameters and pathological and limb functions. Methods Twenty-one New Zealand white rabbits were randomly selected for single stereotactic irradiation on one side of the hind limb to make the model of sciatic nerve radiation injury. The experimental rabbits were scanned with DTI and T2WIIR before irradiation, 1 day after irradiation, and 4 months after irradiation. At the same time, the functional changes of limbs were evaluated. At each time point, 2 rabbits were killed at random for electron microscopy. Results there was no significant difference in DTI parameters between the irradiated side and the control side before irradiation (P 0.05). There was no significant difference in FA value between the irradiated side and the control side 1 day after irradiation (P0. 075), but the FA value of the irradiated side was lower than that of the control side at the 3rd and 4th month after irradiation (P0. 05). On the first day after irradiation, 位 _ () and ADC in the irradiated side were significantly higher than those in the control (P < 0. 05). There was a positive correlation between the FA value and limb function (r = 0. 833, P < 0. 039), while there was a negative correlation between 位 _ () value and limb function (r = 0. 833). There was no correlation between 位 _ (0) and limb function (r = 0. 586- 0. 463a, P < 0. 05). Conclusion the trends of FA and 位 Karabakh over time are consistent with the limb function and pathological changes, and can be used as a reliable method to evaluate the peripheral nerve radiation injury in rabbits.
【作者单位】：广州医科大学附属第一医院放射科;
【基金】：国家自然基金项目(81171800) 广东省科技厅基金项目(2013B021800302)
【分类号】：R445.2;R818

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