不同载荷强度对骨腱结合部结构的影响

发布时间：2018-08-24 12:56

【摘要】：研究目的：目前运动受到越来越广泛的重视,更多的人参与到运动中,而竞技运动的水平也在日益增高,同时不可避免的出现各种跟运动相关的病症,骨腱结合部的损伤是困扰运动训练和人们日常生活的常见损伤,也是体育科研领域的难题,本论文通过不同强度的循环载荷刺激作为干预手段,对骨腱结合部损伤进行研究,确定不会引起骨腱结合部损伤的刺激强度阂值。研究对象与方法：本研究的实验对象为40只成年(18周)雌性新西兰大白兔,体重2.5±0.2kg,随机筛选分为循环载荷CL30(n=8), CL24(n=8), CL18(n=8), CL12(n=8), CL6(n=8),5个强度组,左侧后肢为循环载荷组,右侧后肢为空白对照组CON (n=8),通过循环载荷损伤造模的方法,每次训练刺激2小时(收缩7200次),每周3次,共4周,累积24小时,4周训练结束后,动物处死取材,脱钙,脱水,石蜡包埋,切片,通过HE, Safranin0组织学染色以及VEGF、 bFGF免疫组化染色的方法对髌腱骨腱结合部的组织学变化进行观察,并且利用图像处理软件对所采集的图像进行分析。评价指标有：肌腱细胞密度、纤维软骨带厚度、粘多糖蛋白分布以及VEGF、bFGF免疫组化染色光密度值。结果：4周的循环载荷刺激后,HE染色结果显示,载荷组CL30, CL24, CL12,CL6组与CON组比较,细胞密度显著性增高,CL18组与对照组相比没有显著性差异；CL30和CL24组纤维软骨带厚度与CON组比较明显增厚,有显著性差异。VEGF免疫组化染色：载荷组CL30组VEGF的表达与CON比较显著性升高,CL24、CL18、CL12、CL6与CON组比较没有显著性差异。bFGF免疫组化染色：循环载荷组CL12、CL6组bFGF的表达与CON组比较显著性增高。结论：1.通过4周不同强度的循环载荷刺激,CL3O、CL24组出现了损伤的病理变化,随着运动强度的不同,骨腱结合部发生了相应的结构改变,说明骨腱结合部损伤与运动强度相关。2.CL12组是骨腱结合部损伤发生的阂下强度,CL12、CL6组的循环载荷刺激都不会造成骨腱结合部损伤,以此为以后运动训练和健身的损伤预防提供依据。
[Abstract]:Research objective: at present, more and more people pay attention to sports, more people participate in sports, and the level of competitive sports is increasing day by day. At the same time, there are inevitably various diseases related to sports. The injury of the bone tendon joint is a common injury which puzzles the exercise training and people's daily life, and is also a difficult problem in the field of sports scientific research. In this paper, the injury of the bone tendon joint is studied by the different intensity cyclic load stimulation as the intervention method. Determine the threshold of stimulation intensity that does not cause injury to the bone tendon junction. Subjects and methods: 40 adult (18 weeks) female New Zealand white rabbits, weighing 2.5 卤0.2 kg, were randomly divided into four groups: cyclic load CL30 (NN8), CL24 (NC8), CL18 (NC8), CL12 (NC8), CL6 (NN8), 5 intensity groups, and left hind limb as cyclic loading group. The right hind limb was a blank control group (CON 8). By the method of cyclic load injury, the rats were stimulated for 2 hours (contraction 7200 times), 3 times a week for a total of 4 weeks. After 24 hours and 4 weeks of training, the animals were sacrificed, decalcified and dehydrated. The histological changes of the patellar tendon joint were observed by HE, Safranin0 histological staining and VEGF, bFGF immunohistochemical staining, and the images were analyzed by image processing software. The indexes were as follows: the density of tendon cells, the thickness of fibrochondrocytes, the distribution of mucopolysaccharide protein and the optical density of VEGF,bFGF immunohistochemical staining. Results the cell density of CL30, CL24, CL12,CL6 group was significantly higher than that of CON group. There was no significant difference in cell density between CL18 group and control group. The thickness of fibrochondrocytes in CL30 and CL24 groups was significantly thicker than that in CON group. The expression of VEGF in CL30 group was significantly higher than that in CON group. The expression of bFGF in CL12,CL6 group was significantly higher than that in CON group. Conclusion 1. The pathological changes of injury were observed in the CL3OOG CL24 group after 4 weeks of cyclic load stimulation. With the different intensity of exercise, the corresponding structural changes occurred in the bony tendon junctions. The results showed that the injury of bone tendon joint was related to the intensity of exercise. 2. Group CL12 was the group of bone tendon joint injury. The cyclic load stimulation of group CL12 and CL6 would not cause the injury of bone tendon junction, which would provide the basis for the prevention of injury in later exercise training and fitness.
【学位授予单位】：北京体育大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R87

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