血小板源性生长因子受体α参与成纤维生长因子对辐射诱导人骨髓间充质干细胞损伤的修复作用

发布时间：2018-08-27 19:46

【摘要】：目的:探讨碱性成纤维生长因子(basic fibroblast growth factor,b FGF)对辐射诱导人骨髓间充质干细胞(human bone marrow mesenchymal stem cell,h BMMSC)增殖、成骨分化损伤后的修复作用及所涉及的可能机制。方法:h BMMSC经0、6、12 Gy X射线辐照后,流式细胞术、cell counting kit-8(CCK-8)、Western blot法和茜素红染色法检测辐射对h BMMSC凋亡、增殖、成骨分化的影响;0、1、5、10、20 ng/ml浓度b FGF分别作用于辐射h BM M SC,探索抗辐射保护作用最佳浓度;Western blot法检测血小板源性生长因子受体α(platelet derived grow th factor receptor alpha,PDGFRα)蛋白表达动态变化。结果:辐射后h BM M SC增殖、成骨分化能力下降,b FGF能够修复h BMMSC增殖、成骨分化损伤(P0.05),5 ng/ml浓度作用最显著;仅辐射后早期(24 h)12 Gy组h BMMSC凋亡高于0 Gy组(P0.05),其余时间点辐射组与未辐照组细胞凋亡无差异(P0.05)。辐射后h BMMSC血小板源性生长因子受体-α表达明显下调,b FGF作用后PDGFRα表达上调。结论:辐射对h BMMSC凋亡无显著影响,b FGF对h BMMSC辐射损伤有修复作用,促进其增殖和成骨分化功能的恢复;h BMMSC增殖、成骨分化损伤与辐射诱导PDGFRα表达下调相关。PDGFRα参与b FGF对h BMMSC辐射损伤的修复。
[Abstract]:Aim: to investigate the effect of basic fibroblast growth factor (basic fibroblast growth factor,b FGF) on radiation-induced proliferation of human bone marrow mesenchymal stem cells (human bone marrow mesenchymal stem cell,h BMMSC) and repair of osteogenic differentiation injury. Methods the cell counting kit-8 (CCK-8) Western blot and alizarin red staining were used to detect the apoptosis and proliferation of h BMMSC irradiated with 0: h BMMSC for 12 Gy X-ray. Effects of osteogenic differentiation on the expression of platelet-derived growth factor receptor 伪 (platelet derived grow th factor receptor alpha,PDGFR 伪 protein were detected by Western blot. The effects of B FGF on radiation protection of h BM M SC, were investigated by Western blot. Results: after irradiation, h BM M SC proliferated and osteogenic differentiation ability decreased. B FGF could repair the proliferation of h BMMSC. The effect of 5 ng/ml concentration on osteogenic differentiation injury (P0.05) was the most significant. The apoptosis of BMMSC in 12 Gy group was higher than that in 0 Gy group at the early stage (24 h) (P0.05), but there was no difference between the radiation group and the unirradiated group at other time points (P0.05). The expression of platelet-derived growth factor receptor- 伪 (PDGF-a) in BMMSC was down-regulated after irradiation, and the expression of PDGFR 伪 was up-regulated after exposure to FGF. Conclusion: radiation has no significant effect on the apoptosis of h BMMSC, and B FGF can repair the radiation damage of h BMMSC, and promote the proliferation and osteogenic differentiation of h BMMSC. Osteogenic differentiation injury was associated with down-regulation of radiation-induced PDGFR 伪 expression. PDGFR 伪 was involved in the repair of h BMMSC induced by b FGF.
【作者单位】：第三军医大学西南医院血液科;
【基金】：国家自然基金面上项目(39870046;81270605;30971066;81470324) 第三军医大学临床与基础重大基金(2012XLC03) 重庆市自然基金(2008BA5001) 重庆市研究生教育改革项目(yjg123114) 军队重大医学科研基金(BWS13C018)
【分类号】：R818

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