循环血miR-21在空间辐射监测中的应用潜力
发布时间：2018-09-01 11:14
【摘要】：深空探测中如何在辐射暴露早期用快速、简便的方法进行辐射损伤及风险评估问题,对传统的辐射剂量计提出巨大的挑战。循环血microRNA(miRNA)具有很好的特异性和稳定性,且积极响应外界环境变化,因此,筛选循环血中辐射敏感的miRNA,对开发基于血液的微创生物辐射标志物具有较大的研究意义和应用价值。本文利用X射线对昆明小鼠进行全身照射,24小时后取血,利用miRNA PCR array的方法检测了21种候选miRNA的表达谱水平,发现多种miRNA对辐射有响应,其中miR-21具有较大的变化幅度。利用实时定量PCR分别验证了miR-21在不同射线(X射线、碳离子束、铁离子束)条件下的剂量效应和时间效应。检测了miR-21在相同和不同鼠龄小鼠个体间的本底表达水平。结果表明,miR-21在不同类型射线照射下表达水平都有升高的趋势,特别是对低剂量辐射也具有显著的响应,具有较强的剂量效应关系。在6~72小时内,其表达水平维持在一个稳定的阈值内。miR-21在相同和不同鼠龄小鼠个体间的本底表达水平不具有差异性,在血清样本的保存及RNA的提取过程中具有较强的稳定性。以上特征说明miR-21具有作为生物辐射剂量计并应用于辐射损伤检测的潜力。
[Abstract]:In deep space exploration, the rapid and simple method of radiation damage and risk assessment in the early stage of radiation exposure poses a great challenge to the traditional radiation dose measurement. Circulating blood microRNA (miRNA) has good specificity, stability and positive response to environmental changes. Therefore, screening radiosensitive miRNA, in circulating blood has great significance and application value in the development of microinvasive biomarkers based on blood. In this paper, blood samples were taken from Kunming mice after whole-body irradiation by X-ray for 24 hours. The expression levels of 21 candidate miRNA were detected by miRNA PCR array method. It was found that many kinds of miRNA were responsive to radiation, among which miR-21 had a large range of changes. The dose effect and time effect of miR-21 under different radiation conditions (X-ray, carbon ion beam, iron ion beam) were verified by real-time quantitative PCR. The background expression of miR-21 in mice of the same and different ages was detected. The results showed that the expression level of miR-21 increased under different types of radiation, especially in response to low dose radiation, and had a strong dose-effect relationship. The expression level of miR-21 was maintained within a stable threshold within 6h for 72 hours. The expression level of miR-21 was not different in the same and different mouse aged mice, but had strong stability in the preservation of serum samples and the process of RNA extraction. These characteristics indicate that miR-21 has the potential to be used as a biological dosimeter and to be used in radiation damage detection.
【作者单位】： 中国科学院近代物理研究所甘肃省空间辐射生物学重点实验室;中国科学院大学;
【基金】：国家自然科学基金(31270895) 国家国际科技合作专项(2015DFR30940) 载人航天预先研究项目(040101)
【分类号】：R852.7
本文编号：2217022
[Abstract]:In deep space exploration, the rapid and simple method of radiation damage and risk assessment in the early stage of radiation exposure poses a great challenge to the traditional radiation dose measurement. Circulating blood microRNA (miRNA) has good specificity, stability and positive response to environmental changes. Therefore, screening radiosensitive miRNA, in circulating blood has great significance and application value in the development of microinvasive biomarkers based on blood. In this paper, blood samples were taken from Kunming mice after whole-body irradiation by X-ray for 24 hours. The expression levels of 21 candidate miRNA were detected by miRNA PCR array method. It was found that many kinds of miRNA were responsive to radiation, among which miR-21 had a large range of changes. The dose effect and time effect of miR-21 under different radiation conditions (X-ray, carbon ion beam, iron ion beam) were verified by real-time quantitative PCR. The background expression of miR-21 in mice of the same and different ages was detected. The results showed that the expression level of miR-21 increased under different types of radiation, especially in response to low dose radiation, and had a strong dose-effect relationship. The expression level of miR-21 was maintained within a stable threshold within 6h for 72 hours. The expression level of miR-21 was not different in the same and different mouse aged mice, but had strong stability in the preservation of serum samples and the process of RNA extraction. These characteristics indicate that miR-21 has the potential to be used as a biological dosimeter and to be used in radiation damage detection.
【作者单位】： 中国科学院近代物理研究所甘肃省空间辐射生物学重点实验室;中国科学院大学;
【基金】：国家自然科学基金(31270895) 国家国际科技合作专项(2015DFR30940) 载人航天预先研究项目(040101)
【分类号】：R852.7
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