正常及挫伤脊髓血管的三维直视化:同步辐射相衬成像

发布时间：2018-09-03 06:10

【摘要】：背景:同步辐射相位衬度成像凭借硬X射线光源的高准直、高相干等特性,其能大幅度提高对软骨细胞及微血管的空间分辨能力。目的:利用高空间分辨率同步辐射相衬成像技术观察大鼠正常及急性损伤脊髓的微血管的形态变化。方法:雄性SD大鼠分为2组:实验组以改良ALLEN’s打击法制作大鼠急性脊髓挫伤模型;伪手术组大鼠只行椎板减压术,不打击脊髓作为正常对照。术后第1天取正常及伤段脊髓标本经甲醛-水杨酸甲酯序贯处理48 h,标本于上海光源BL13W1硬X射线站进行扫描和成像。采集数据以利用VG Studio Max 2.1软件包进行3D图形重建及血管量化分析。结果与结论:相位衬度成像以特有的血管边缘增强效应直观呈现脊髓微血管形态,其结合CT技术(相衬断层成像)从三维视角对脊髓微血管进行直观成像,急性脊髓挫伤后神经组织的破坏伴随着严重的血管结构的损毁,组织损伤及血供的缺失在髓内由中央区向头、尾两侧呈梭性蔓延。三维血管量化数据显示脊髓挫伤后微血管数目及血管灌注容积急剧减少(P0.01)。结果表明无需血管造影的条件下,相衬断层成像可作为一种新的有潜力的超高分辨率可视化技术用于脊髓微血管3D结构成像及量化分析。
[Abstract]:Background: synchrotron radiation phase contrast imaging can greatly improve the spatial resolution of chondrocytes and microvessels by virtue of the high collimation and coherence of hard X-ray sources. Aim: to observe the morphologic changes of microvessels in normal and acute injured spinal cord by high spatial resolution synchrotron radiation contrast imaging. Methods: male SD rats were divided into two groups: the experimental group was made into acute spinal cord contusion model by modified ALLEN's attack method, and the pseudo-operation group was treated with laminar decompression only, without striking the spinal cord as the normal control. The normal and injured spinal cord samples were sequentially treated with formaldehyde-methyl salicylate for 48 hours on the first day after operation. The specimens were scanned and imaged at BL13W1 hard X-ray station of Shanghai Light Source. Data were collected for 3D graphics reconstruction and vascular quantitative analysis using VG Studio Max 2.1 software package. Results and conclusion: phase contrast imaging can visualize the morphology of spinal microvessels with the characteristic edge enhancement effect. The phase contrast imaging combined with CT (phase contrast tomography) is used to visualize the spinal microvessels from a three-dimensional perspective. The damage of nerve tissue after acute spinal cord contusion was accompanied by serious damage of vascular structure. The injury of tissue and the loss of blood supply spread from the central area to the head of the medulla and the two sides of the tail spread fusiform. Three dimensional vascular quantitative data showed that the number of microvessels and the volume of vascular perfusion decreased significantly after spinal cord contusion (P0.01). The results show that phase contrast tomography can be used as a potential ultra-high resolution visualization technique for 3D structure imaging and quantitative analysis of spinal microvessels without angiography.
【作者单位】：中南大学湘雅医院脊柱外科;中南大学湘雅医院运动医学科运动医学研究中心;
【基金】：国家自然科学基金(81301542,81371956)~~
【分类号】：R651.2;R816.1

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