运动对成长期骨骼肌神经源性诱导因子Agrin及其受体MuSK表达的影响

发布时间：2018-09-07 21:27

【摘要】：目的:本研究通过分析神经源性诱导因子Agrin及其受体-肌特异性受体酪氨酸激酶Mu SK在成长期(离乳-性成熟)骨骼肌的表达水平,探讨其在神经肌肉连接(NMJ)发育中的作用及早期运动干预的影响。方法:出生18天SD雄性大鼠130只随机分为运动组50只、悬吊组和对照组各40只,运动组和悬吊组分别进行运动增强(跑台运动)和运动减弱(尾部悬吊)干预。对照组3周龄时,各组4周龄、5周龄、6周龄、8周龄时分别取材10只。测定并比较正常发育(3~8周龄)及运动干预下腓肠肌Agrin和Mu SKm RNA水平(RT-PCR法)及腓肠肌Mu SK蛋白水平(ELISA法)的差异。结果:(1)对照组的Agrin m RNA在4周龄较3周龄表达减少(P0.01),5周龄再次出现表达高峰(P0.01),在4周龄后Mu SK m RNA表达减少(P0.01),6周龄再次出现Mu SK m RNA表达高峰(P0.01);运动组Mu SK m RNA表达水平在5周龄时显著高于对照组(P0.01),而悬吊组Mu SK m RNA表达水平在各周龄均低于对照组(P0.05,P0.01)。(2)运动组Mu SK蛋白水平在各周龄组均高于对照组(P0.05);悬吊组在4周龄时低于对照组(P0.05)。结论:成长期腓肠肌Agrin及其受体Mu SK的表达具有一定的时间规律,在出生3~4周(离乳前后)呈现逐渐减少的趋势,5~6周(青春期)再次出现表达高峰;在此期间进行适当的运动训练可以促使Agrin及其受体Mu SK表达增多,这可能作为一种机制,促进NMJ在成长期的发育过程。
[Abstract]:Objective: to investigate the expression of neurogenic inducible factor (Agrin) and its receptor-specific receptor tyrosine kinase (Mu SK) in adult skeletal muscle. To explore its role in the development of neuromuscular junction (NMJ) and the effect of early exercise intervention. Methods: one hundred and thirty male SD rats were randomly divided into two groups: exercise group (n = 50), suspension group (n = 40) and control group (n = 40). In the control group, 10 rats were collected at the age of 3 weeks, 4 weeks, 5 weeks, 6 weeks and 8 weeks. The levels of Agrin and Mu SKm RNA in gastrocnemius muscle (RT-PCR method) and Mu SK protein level in gastrocnemius muscle (ELISA method) were measured and compared under normal development (3 ~ 8 weeks old) and exercise intervention. Results: (1) the expression of Agrin m RNA in the control group was decreased at the age of 4 weeks compared with that in the age of 3 weeks (P0.01). The peak expression of Mu SK m RNA was again found in the control group at the age of 5 weeks (P0.01), and in the control group at the age of 4 weeks (P0. 01) and at the age of 6 weeks (P0. 01), and in the exercise group, the expression of Mu SK m RNA appeared again (P0. 01). The expression level of Mu SK m RNA in the suspension group was significantly higher than that in the control group at the age of 5 weeks (P0.01), while the Mu SK protein level in the exercise group was significantly higher than that in the control group at the age of 5 weeks (P0.05), and in the suspension group was lower than that in the control group at 4 weeks of age (P0.05). Conclusion: the expression of Agrin and its receptor Mu SK in gastrocnemius muscle has a certain time regular pattern. The expression peak of Agrin and its receptor Mu SK in gastrocnemius muscle is decreasing gradually at 3 ~ 4 weeks (before and after the birth), and it appears again at 5 ~ 6 weeks (puberty). During this period, proper exercise training can increase the expression of Agrin and its receptor Mu SK, which may be used as a mechanism to promote the development of NMJ in the process of development.
【作者单位】：国家体育总局运动医学研究所;
【基金】：国家体育总局体育科学研究所基本科研业务费课题13-11资助
【分类号】：R87

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