低氧运动对肥胖小鼠脂肪UCP-1和PGC-1α表达的影响

发布时间：2018-10-11 19:17

【摘要】：目的:探究低氧和运动干预对肥胖小鼠棕色脂肪生成及活化的影响。方法:50只C57BL/6J小鼠随机分为普通对照组(ND组)、高脂膳食组(HD组),HD组建立肥胖模型成功小鼠随机分为常氧安静组(OB)、常氧运动组(E)、低氧安静组(H)和低氧运动组(EH)。4周后,测试各组小鼠体脂率和棕色脂肪含量并计算棕色脂肪占总脂肪比率(B/F%);RT-PCR法检测白色、棕色脂肪中解偶联蛋白1(UCP-1)和氧化物酶体增殖物激活受体γ辅助激活因子α(PGC-1α)m RNA表达水平,western blot法测定白色、棕色脂肪中PGC-1α的蛋白含量。结果:干预后,(1)E、H及EH组体脂率较OB组非常显著性下降,H、EH组B/F%较OB组非常显著性上升。(2)白色脂肪中,H、EH组的UCP-1 m RNA水平较OB组显著升高,E、H、EH组的PGC-1αm RNA水平较OB组显著升高;棕色脂肪中,E、EH组的UCP-1 m RNA水平较OB组显著下降,E、H、EH组的PGC-1αm RNA水平较OB组显著下降。(3)与OB组相比,E组白色脂肪中PGC-1α蛋白含量有升高趋势,EH组有下降趋势,但差异均无显著性;E、H、EH组棕色脂肪中PGC-1α蛋白含量均高于OB组(P0.01,P0.05)。结论:低氧运动可促进白色脂肪棕色化,提高肥胖机体棕色脂肪活性。
[Abstract]:Objective: to investigate the effects of hypoxia and exercise intervention on brown fat production and activation in obese mice. Methods: fifty C57BL/6J mice were randomly divided into normal control group (ND group) and high-fat diet group (HD group,), HD group). The obese mice were randomly divided into (OB), normoxic exercise group (OB), normoxic exercise group), (E), hypoxia quiet group (H) and hypoxic exercise group (EH). 4 weeks later). The body fat rate and brown fat content in each group were measured and the ratio of brown fat to total fat (B / F%) was calculated. The expression of uncoupling protein 1 (UCP-1) and PGC-1 伪 (PGC-1 伪) m RNA) in brown fat was determined by, western blot method. Results: after intervention, (1) the percentage of body fat in EH and EH groups was significantly lower than that in OB group, and the B / F% in EH group was significantly higher than that in OB group. (2) in white fat, the UCP-1 m RNA level in HG EH group was significantly higher than that in OB group, and the PGC-1 伪 m RNA level in EH group was significantly higher than that in OB group. In brown fat, the level of UCP-1 m RNA in EH group was significantly lower than that in OB group, and the PGC-1 伪 m RNA level in EH group was significantly lower than that in OB group. (3) compared with OB group, the content of PGC-1 伪 protein in white fat in E group was increased, and that in EH group was decreased. The content of PGC-1 伪 protein in brown fat of EHH group was higher than that of OB group (P0.01 P 0.05). Conclusion: hypoxia exercise can promote the browning of white fat and increase the activity of brown fat in obese body.
【作者单位】：北京体育大学运动人体科学学院;北京体育大学科学研究中心;河北省体育科学研究所;
【基金】：2013年国家级大学生创新创业训练计划项目(2013A58) 2014年国家体育总局重点研究领域公关课题(2014B037) 中央高校基本科研业务费专项资金资助课题(2015SYS010)
【分类号】：R87

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