早期运动训练对脊髓损伤大鼠后肢运动功能影响及相关机制研究

发布时间：2018-10-14 15:35

【摘要】：目的:探讨早期运动训练对脊髓损伤大鼠后肢运动功能的影响及其相关机制。方法:成年SD雄性大鼠75只,采用改良钳夹法建立大鼠T10损伤模型。术后随机分为假手术组(A组)、脊髓损伤组(B组)、脊髓损伤+跑台训练组(C组),术前3天及术后第2、3、7、14天采用BBB行为功能评定量表对大鼠后肢运动功能进行评分。术后第2、3、5、7天取损伤中心头尾端约1cm的脊髓组织进行脊髓含水量的测定,术后第3天行免疫组化检测脊髓组织损伤段半胱氨酸蛋白酶3(caspase-3)和髓过氧化物酶(MPO)的表达情况,行HE染色观察脊髓组织病理学改变。结果:1术前各组大鼠后肢运动功能评分均为21分,术后B组和C组大鼠评分随着时间的延长而逐渐升高,第7天时3组之间差异有显著性意义(P0.05)。2A组大鼠脊髓含水量基本不变,脊髓损伤后第3天,水肿达到高峰,随后逐渐下降,到术后第7天脊髓含水量仍高于A组,且各个时间点差异均有显著性意义(P0.05)。3术后第3天A组大鼠caspase-3少量表达,MPO基本不表达,B组大鼠caspase-3和MPO的表达均高于C组,3组相比差异均有显著性意义(P0.05)。4B组脊髓组织病理学改变较C组重。结论:早期运动训练可以促进脊髓损伤大鼠后肢运动功能的恢复,其机制可能与减轻脊髓损伤后水肿,减少细胞凋亡和炎症反应有关。
[Abstract]:Objective: to investigate the effect of early exercise training on the motor function of hindlimb in rats with spinal cord injury (sci). Methods: 75 adult SD male rats were used to establish T 10 injury model by modified clamp method. The rats were randomly divided into three groups: sham operation group (group A), spinal cord injury group (group B), spinal cord injury treadmill training group (group C), 3 days before operation and 714 days after operation. The motor function of hind limbs was evaluated with BBB behavioral function assessment scale. The water content of the spinal cord was measured at the end of the head and tail of the injured center on the 7th day after operation. The expression of cysteine protease 3 (caspase-3) and myeloperoxidase (MPO) in the injured spinal cord was detected by immunohistochemistry on the third day after operation, and the expression of cysteine protease 3 (caspase-3) and myeloperoxidase (MPO) in the injured spinal cord were detected by immunohistochemistry. The histopathological changes of spinal cord were observed by HE staining. Results: 1the scores of hindlimb motor function were 21 in each group before operation. The scores of rats in group B and group C gradually increased with time after operation, and there was significant difference between the three groups on the 7th day (P0.05). The water content of spinal cord in group 2A was almost unchanged. On the 3rd day after spinal cord injury, the edema reached its peak and then decreased gradually, and the water content of spinal cord was still higher than that of group A on the 7th day after operation. There was significant difference at all time points (P0.05). 3 on the 3rd day after operation, the expression of caspase-3 in group A was small, but the expression of MPO was not. The expression of caspase-3 and MPO in group B was higher than that in group C, and there was significant difference among the three groups (P0.05). 4The expression of caspase-3 and MPO in group B was significantly higher than that in group C (P0.05). The pathological changes were more serious than those in group C. Conclusion: early exercise training can promote the recovery of hindlimb motor function in rats with spinal cord injury, and its mechanism may be related to the reduction of edema, apoptosis and inflammation after spinal cord injury.
【作者单位】：南方医科大学珠江医院门诊部;
【基金】：广东省科技计划项目(2010B080701020)
【分类号】：R87

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