西咪替丁对急性照射小鼠存活率及造血系统改变的影响

发布时间：2018-11-20 07:03

【摘要】：目的观察西咪替丁对急性照射小鼠存活率及造血系统改变的影响。方法采用137Csγ射线全身照射小鼠,两次实验的照射剂量分别为6.0Gy和8.0Gy,剂量率均为1.01Gy/min。每次实验取健康雄性C57BL/6小鼠60只,按体重随机分为空白对照组、模型对照组、阳性药组(523)及33.3、100、300mg/kg西咪替丁组,每组10只。西咪替丁各剂量组于照射前6d灌胃给药,每天1次,照射后5h给药1次;阳性药组于照射前1d灌胃给药1次,照射后5h给药1次。检测8.0Gy照射后30d小鼠存活率,以及6.0Gy照射后30d小鼠外周血象、骨髓DNA含量和骨髓嗜多染红细胞微核率。结果8.0Gy照射后,模型对照组小鼠于第21天全部死亡,西咪替丁低、中、高剂量组30d存活率分别为50%、20%和30%;6.0Gy照射后第30天,与空白对照组比较,各照射组小鼠外周血白细胞明显降低(P0.01),骨髓嗜多染红细胞微核率明显升高(P0.05),骨髓DNA含量明显降低(P0.05);与模型对照组比较,西咪替丁高剂量组外周血白细胞明显升高(P0.01),西咪替丁各剂量组骨髓DNA含量明显升高(P0.01,P0.05),而骨髓嗜多染红细胞微核率明显降低(P0.01,P0.05)并趋于正常。结论西咪替丁能提高急性照射小鼠30d存活率,且对其造血系统有较好的保护作用。
[Abstract]:Objective to observe the effect of cimetidine on survival rate and hematopoietic system of acute irradiated mice. Methods the mice were irradiated with 137Cs 纬 -ray in whole body. The dose of 6.0Gy and 8.0 Gy in the two experiments was 1.01Gy / min. Sixty healthy male C57BL/6 mice were randomly divided into blank control group, model control group, positive drug group (523) and 33.3100300mg/kg cimetidine group. Cimetidine was administered by gavage 6 days before irradiation, once a day, 5 hours after irradiation, and 1 time by stomach on 1 day before irradiation in the positive drug group, and once at 5 hours after irradiation in the positive drug group. The survival rate of mice 30 days after 8.0Gy irradiation, peripheral blood picture, bone marrow DNA content and micronucleus rate of bone marrow polychromatic erythrocytes were measured. Results after 8.0Gy irradiation, all the mice in the model control group died on the 21st day, and cimetidine was low. The 30-day survival rate of the high dose group was 50% and 30%, respectively. On the 30th day after 6.0Gy irradiation, compared with the blank control group, the peripheral white blood cells in each irradiation group decreased significantly (P0.01), the micronucleus rate of bone marrow polychromatic erythrocytes increased significantly (P0.05), and the content of bone marrow DNA decreased significantly (P0.05). Compared with the model control group, the peripheral blood leukocytes in cimetidine high dose group increased significantly (P0.01), and the DNA content in bone marrow of cimetidine group increased significantly (P0.01P05). The micronucleus rate of bone marrow polychromatic erythrocytes decreased significantly (P 0.01 P 0.05) and tended to normal. Conclusion cimetidine can improve the 30 day survival rate of acute irradiation mice and has a better protective effect on hematopoietic system.
【作者单位】：海军医学研究所辐射防护效应研究室;
【基金】：国家科技重大专项军特药保密专项(2014ZX09J14103-07B)~~
【分类号】：R818

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