大鼠脑损伤后EPO及其受体表达与损伤时间的关系

发布时间：2018-11-27 07:05

【摘要】：目的研究大鼠脑损伤后脑组织中促红细胞生成素(erythropoietin,EPO)及其受体(erythropoietin receptor,EPOR)的表达与损伤时间的关系。方法 72只SD大鼠随机分为对照组(36只)、脑损伤组(36只),在建模后1、2、4、8、12、24 h每组各取6只大鼠处死,取挫伤部位脑组织,应用实时荧光定量PCR和Western印迹法检测不同时间点EPO以及EPOR的mRNA表达和蛋白表达情况。结果伤后24h内,EPO及EPOR的表达随损伤时间的增加而呈上升趋势。EPO的mRNA及蛋白表达与损伤时间之间存在相关性,其相关系数分别为0.875、0.911(P0.05);EPOR的mRNA及蛋白表达与损伤时间之间存在相关性,其相关系数分别为0.936、0.905(P0.05)。结论大鼠颅脑损伤早期EPO及EPOR的表达呈逐步上升趋势,与损伤时间具有相关性,可以作为脑损伤时间推断的参考。
[Abstract]:Objective to study the relationship between the expression of erythropoietin (erythropoietin,EPO) and erythropoietin receptor (erythropoietin receptor,EPOR) and injury time after brain injury in rats. Methods Seventy-two SD rats were randomly divided into control group (36 rats) and brain injury group (36 rats). Six rats in each group were killed and the contusion brain tissue was taken out in each group. The expression of mRNA and protein in EPO and EPOR at different time points were detected by real-time fluorescence quantitative PCR and Western blotting. Results within 24 hours after injury, the expression of EPO and EPOR increased with the increase of injury time. There was a correlation between the expression of mRNA and protein of EPO and the time of injury, and the correlation coefficient was 0.875 卤0.911 (P0.05). There was a correlation between the expression of mRNA and protein in EPOR and the time of injury, the correlation coefficient was 0.936 ~ 0.905 (P0.05). Conclusion the expression of EPO and EPOR in the early stage of craniocerebral injury in rats showed an increasing trend, which was correlated with the time of brain injury, and could be used as a reference for estimating the time of brain injury.
【作者单位】：铁道警察学院公安技术系;
【基金】：公安部科技应用创新项目(2012YYCXTDGZ138)
【分类号】：D919

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