不同强度的间歇跑台训练对大鼠心肌能量代谢相关指标的影响

发布时间：2018-12-16 10:40

【摘要】：1研究目的通过对大鼠心肌能量代谢相关指标的测定,探讨不同强度的间歇跑台训练对大鼠能量代谢代谢相关酶和氧化基质的影响,以期为运动训练强度的制定提供理论支持。 2实验方法选取4周龄雄性wistar大鼠70只(由山东鲁抗医药股份有限公司提供)，平均体重为67.99g±5.83g,随机分成7组,每组10只，即安静对照组和运动组，运动组按运动训练强度分为65%组、70%组、75%组、80%组、85%组和90%组。跑台训练9周，，每周训练6天，每组训练分3次，每次不低于10分钟，中间间隔30分钟，最后一次运动后24h，毁脊髓法处死后解剖，迅速取出心肌，在生理盐水中漂洗干净，置-84℃冰箱保存待测。制备组织匀浆分别测定大鼠心肌肌糖原含量、琥珀酸脱氢酶（SDH）、苹果酸脱氢酶（MDH）、乳酸脱氢酶（LDH）、Ca~(2+)ATP酶、乳酸含量。所有数据用SPSS17.0软件进行统计学处理。 3实验结果 3.1大鼠心肌肌糖原含量的比较 80%组和85%组的心肌肌糖原含量显著高于对照组（P＜0.05）；80%组和85%组的心肌肌糖原含量显著高于65%组（P＜0.05）；对照组、65%组、70%组、75%组和90%组之间心肌肌糖原含量无显著性差异。 3.2大鼠心肌SDH活性的比较 90%组的心肌SDH活性显著高于对照组和65%组（P＜0.05）；对照组、65%组、70%组、75%组、80%组与85%组之间心肌SDH活性无统计学差异。 3.3大鼠心肌MDH活性的比较 65%组、70%组、75%组、80%组、85%组与90%组的心肌MDH活性均显著高于对照组（P＜0.05）；85%组的心肌MDH活性最高，但运动组之间心肌MDH活性无显著性差异。3.4大鼠心肌LDH活性的比较 65%组、70%组、75%组、80%组、85%组与90%组的心肌LDH活性均显著高于对照组（P＜0.05）；90%组的心肌LDH活性显著高于65%组和70%组（P＜0.05）；65%组、70%组、75%组、80%组与85%组的心肌LDH活性有增大的趋势但相互之间无显著差异性。 3.5大鼠心肌Ca~(2+)ATP酶活性的比较65%组、70%组、75%组、80%组与85%组的心肌Ca~(2+)ATP酶活性显著高于对照组（P＜0.05）；85%组的心肌Ca~(2+)ATP酶活性最高；90%组心肌Ca~(2+)ATP酶略高于对照组，但无显著性差异。 3.6大鼠心肌LD含量的比较90%组LD含量显著高于对照组（P＜0.05）；其它各组之间无显著性差异。 4结论 4.165%VO_2max、70%VO_2max和75%VO_2max的中等强度的跑台训练不能显著地增加大鼠心肌肌糖原的含量，而85%VO_2max强度的长期训练最能显著地增加大鼠心肌肌糖原含量。90%VO_2max的长期跑台训练不利于大鼠心肌肌糖原的恢复及储备。 4.2不同强度的跑台训练均能显著提高大鼠心肌有氧代谢酶SDH、MDH的数量及活性，85%VO_2max的长期跑台训练最能提高大鼠心肌有氧代谢酶的活性，有利于心肌有氧代谢活动的发展。 4.3不同强度的跑台训练均能显著提高大鼠心肌无氧代谢酶LDH的活性，90%VO_2maxLDH活性最高。90%VO_2max的长期跑台训练能使大鼠心肌产生肌糖原的无氧酵解供能，产生LD，且24小时后不能完全恢复。 4.465%VO_2max、70%VO_2max、75%VO_2max、80%VO_2max和85%VO_2max的长期跑台运动能显著提高大鼠心肌Ca~(2+)ATP酶的活性，85%VO_2maxCa~(2+)ATP酶的活性最高。90%VO_2max的长期跑台训练导致大鼠心肌代谢紊乱，不利于Ca~(2+)ATP酶的恢复。
[Abstract]:1 Purpose of study In order to provide theoretical support for the development of the intensity of exercise training, the effects of the intermittent running of different intensity on the metabolism of energy metabolism and the oxidative matrix of the rats were discussed by the determination of the related indexes of energy metabolism in the myocardium of the rats. hold. 2 The average body weight was 67. 99g (5.83g), and the average body weight was 67. 99g (5.83g), which was divided into 7 groups. In group and exercise group, the exercise group was divided into 65% group, 70% group, 75% group, 80% group and 85% group according to the exercise training intensity. and training for 9 weeks, training for 6 days per week, 3 times for each group, not less than 10 minutes at a time, 30 minutes for the middle interval, 24 hours after the last exercise, after the spinal cord is killed and then dissected, the myocardium is quickly removed, rinsed in normal saline, and the ice is set at -84 DEG C. The glycogen content, the succinate dehydrogenase (SDH), the malic acid dehydrogenase (MDH), the lactate dehydrogenase (LDH), the Ca ~ (2 +) ATPase in the myocardium of the rat were measured., lactic acid content. All data is performed with the SPSS17.0 software statistical processing. 3 Experimental results 3. 1 rat The glycogen content of the myocardium in the 80% and 85% group was significantly higher than that in the control group (P <0.05), and the glycogen content of the myocardial muscle in the 80% and the 85% group was significantly higher than that of the control group (P <0.05). Myocardium in 5% group (P <0.05); control group, 65% group, 70% group, 75% group and 90% group There was no significant difference in the content of glycogen in the muscle.. Compared with the control group and the 65% group (P <0.05), the activity of SDH in the myocardium of the rats was significantly higher than that in the control group (P <0.05), the control group, the 65% group, the 70% group, the 75% group, the 80% group and the 85% group. There was no statistical difference between the activity of SDH and SDH between the two groups.. 3. Compared with the control group (P <0), the activity of MDH in the myocardium of the rats in 65%, 70%, 75%, 80%, 85% and 90% were significantly higher than those in the control group (P <0).. 05) The MMDH activity in the 85% group was the highest, but the activity of MDH between the groups was not significant. A comparison of the activity of LDH in 3. 4 rats was 65%, 70%, 75%, 80%, 85% and 90% of the myocardium. The activity of LDH was significantly higher than that in the control group (P <0.05); the activity of LDH in the 90% group was significantly higher than that in the 65% group and 70% group (P <0.05); 65% group, 70% group, 75% group, 80% group and 85% group of myocardium L Compared with the control group (P <0.05), the activity of Ca ~ (2 +) ATPase in the myocardium of the rats was significantly higher than that in the control group (P <0.05). The activity of Ca ~ (2 +) ATPase in the group was higher than that in the control group (P <0.05). The activity of Ca ~ (2 +) ATPase in the group of 85% was the highest, and the Ca ~ (2 +) ATPase activity in the group was higher than that of the control group (P <0.05). ~ (2 +) ATPase was slightly higher than that in the control group, but there was no significant difference. in control 4. The long-term training of the intensity of 85% VO _ 2max could significantly increase the glycogen content of the rat cardiac muscle, while the long-term training of 85% VO _ 2max could increase the glycogen content of the myocardium of the rat significantly. The long-term training of O _ 2max is not conducive to the recovery and storage of the glycogen in the myocardium of the rat. The activity of the aerobic metabolizing enzyme in the myocardium of the rat can be improved, which is beneficial to the development of the activity of the aerobic metabolism of the myocardium. The results showed that the activity of Ca ~ (2 +) ATPase and the activity of 85% VO _ 2maxCa ~ (2 +) ATPase were the highest. The activity of Ca ~ (2 +) ATPase in rat myocardium was significantly increased by the long-term treadmill exercise of 4. 465% VO _ 2max, 70% VO _ 2max, 75% VO _ 2max, 80% VO _ 2max, and 85% VO _ 2max. The activity of 85% VO _ 2maxCa ~ (2 +) ATPase was the highest. 90% VO _ 2max
【学位授予单位】：曲阜师范大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R87

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