蛋白激酶C对晚期运动预处理心肌保护效应中缝隙连接蛋白43表达的影响

发布时间：2018-12-19 11:27

【摘要】：目的:探讨蛋白激酶C(PKC)与大鼠心肌缝隙连接蛋白43(Cx43)在晚期运动预处理(LEP)心肌保护效应中的关系及相互影响。方法:48只SD大鼠分为对照组(C组)、力竭运动组(EE组)、晚期运动预处理组(LEP组)、白屈菜赤碱+晚期运动预处理组(CHE+LEP组)、晚期运动预处理+力竭运动组(LEP+EE组)和白屈菜赤碱+晚期运动预处理+力竭运动组(CHE+LEP+EE组),每组8只。在进行LEP或/和PKC特异性抑制剂CHE干预后运动至力竭,观察、检测心肌Cx43 m RNA和蛋白的分布情况及表达变化。结果:⑴各组Cx43 m RNA原位杂交信号未见明显差异,实时荧光定量PCR结果亦无显著差异。⑵CHE+LEP组Cx43免疫阳性表达较LEP组减弱,CHE+LEP+EE组Cx43免疫阳性表达较LEP+EE组减弱;CHE+LEP组Cx43蛋白表达较LEP组显著降低,CHE+LEP+EE组Cx43蛋白表达较LEP+EE组显著降低。结论:在晚期运动预处理心肌保护效应信号转导通路中,PKC是心肌Cx43的上游中介物质。
[Abstract]:Aim: to investigate the relationship between protein kinase C (PKC) (PKK) and gap junction protein 43 (Cx43) in rat myocardium during late exercise preconditioning (LEP) myocardial protection. Methods: 48 SD rats were divided into three groups: control group (group C), exhaustive exercise group (EE group), advanced exercise preconditioning group (LEP group), and late exercise pretreatment group (CHE LEP group). There were 8 rats in each group (LEP EE group) and (CHE LEP EE group (8 rats in each group). After the intervention of LEP or / and CHE, a specific inhibitor of PKC, the distribution and expression of Cx43 m RNA and protein in myocardium were observed. Results: (1) there was no significant difference in Cx43 m RNA in situ hybridization signal, and no significant difference was found in real-time fluorescence quantitative PCR. The positive expression of Cx43 in the 2CHE LEP group was lower than that in the LEP group and the Cx43 immunoreactive expression in the, CHE LEP EE group was lower than that in the LEP EE group. Cx43 protein expression in CHE LEP group was significantly lower than that in LEP group. Cx43 protein expression in, CHE LEP EE group was significantly lower than that in LEP EE group. Conclusion: in the signal transduction pathway of myocardial protective effect of late exercise preconditioning, PKC is the upstream mediator of myocardial Cx43.
【作者单位】：曲阜师范大学体育科学学院;
【基金】：中国博士后科学基金曲阜师范大学博士科研启动基金
【分类号】：R87

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