力竭运动对大鼠骨骼肌NOS活性的影响

发布时间：2018-12-27 13:01

【摘要】：一氧化氮（NO）自由基为一小分子气体，以往对其生物学功能认识不清。自从发现能舒张血管的一种内皮细胞松弛因子（endothelium-derivedrelaxingfactor,EDRF）的化学本质即一氧化氮自由基以来，一氧化氮自由基的研究进入了一个新的时期。近几十年来，一氧化氮自由基的研究进展影响着对许多生命活动本质的认识，涉及许多学科，，包括医学和药学等等，近年来更是扩展到体育学科。不同运动负荷对一氧化氮及一氧化氮合酶的影响研究成为其中的热点。不同研究方向的学者们都共同投入到对一氧化氮的研究中来，试图从各个角度来揭示一氧化氮的奥秘。虽然运动医学者们在一氧化氮、运动与骨骼肌方面做了大量工作，但存有争议的问题仍然很多。涉及到NOS的三种亚型与运动关系的研究更是争议颇大，未有一个定论存在。现有研究表明，运动导致NO生成，NOS活性发生变化，而力竭运动导致NOS活性升高的具体亚型尚不清楚，并且也有研究表明，骨骼肌中含有大量的nNOS，因此，本研究推测，力竭运动导致骨骼肌NOS活性升高主要是有nNOS活性升高所致。研究方法：将32只大鼠随机分成对照（C组）与训练（E组）两组，进行跑台力竭运动，于运动结束后即刻取样。研究结果：（1）力竭训练后，大鼠血清CK活性升高33.81%（P0.05），而血清LDH下降7.59%，无显著性差异（P0.05）。（2）力竭训练后，大鼠血清NO显著增加了16%（P0.05）。（3）力竭训练后，大鼠骨骼肌MDA含量非常显著性增加（P0.01），骨骼肌SOD活性显著下降（P0.05）。（4）力竭训练后，总NOS活性增加，有显著性差异，大鼠骨骼肌nNOS活性非常显著性升高（P0.01），iNOS活性升高，但无显著性差异。结论：（1）力竭运动导血清CK活性增高。（2）力竭运动导致NOS活性增加，其根源在于nNOS活性增加，iNOS无变化；由于NOS活性增加，导致NO生成也增加。（3）力竭运动导致脂质过氧化程度加强。
[Abstract]:Nitric oxide (NO) radical is a small molecule gas and its biological function was not well understood in the past. Since the discovery of the chemical nature of an endothelial cell relaxation factor (endothelium-derivedrelaxingfactor,EDRF), the nitric oxide free radical, which can relax blood vessels, the study of nitric oxide free radical has entered a new stage. In recent decades, the research progress of nitric oxide free radicals has affected the understanding of the nature of many life activities, involving many disciplines, including medicine and pharmacy, etc. In recent years, it has been extended to physical education. The effects of different exercise loads on nitric oxide (no) and nitric oxide synthase (NOS) have become a hot topic. Scholars from different research fields have been involved in the study of nitric oxide, trying to reveal the mystery of nitric oxide from various angles. Although sports medicine has done a lot of work on nitric oxide, exercise and skeletal muscle, there are still many controversial issues. The research on the relationship between the three subtypes of NOS and sport is more controversial, and there is no conclusion. Current studies have shown that exercise causes NO production and changes in NOS activity, while the specific subtypes of exhaustive exercise that lead to increased NOS activity are not clear. Some studies have also shown that skeletal muscle contains a large number of nNOS,. The increase of NOS activity in skeletal muscle caused by exhaustive exercise was mainly due to the increase of nNOS activity. Methods: 32 rats were randomly divided into two groups: control group (group C) and training group (group E). Results: (1) after exhaustive training, the serum CK activity increased by 33.81% (P0.05), while the serum LDH decreased 7.59, there was no significant difference (P0.05). (2) after exhaustive training, serum NO increased significantly by 16% (P0.05). (3) after exhaustive training, the MDA content of skeletal muscle increased significantly (P0.01), and the SOD activity of skeletal muscle decreased significantly (P0.05). (4) after exhaustive training, the total NOS activity increased significantly, and the nNOS activity of skeletal muscle increased significantly (P0.01), iNOS activity, but there was no significant difference). Conclusion: (1) the activity of CK in exhaustive exercise induced serum increased. (2) exhaustive exercise resulted in the increase of NOS activity, which was caused by the increase of nNOS activity, but no change in iNOS activity, and the increase of NOS activity led to the increase of NO production. (3) exhaustive exercise increased the degree of lipid peroxidation.
【学位授予单位】：湖南大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：G804.2

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