CT引导下盐酸溶液化学消融实验研究
发布时间：2019-05-23 10:00
【摘要】：目的 1、研究6mol/l盐酸溶液局部注射对新西兰大白兔机体的影响，探讨6mol/l盐酸溶液作为化学消融剂使用的可行性。 2、研究不同剂量盐酸溶液作用于新西兰大白兔肝脏、背部肌肉消融范围的大小，观察消融后不同阶段组织坏死情况及周围炎性反应，明确消融区域组织学变化，总结机体对盐酸消融区域修复规律。 材料和方法 1、在CT引导下将6mol/l盐酸溶液用微量泵缓慢注入4只新西兰大白兔前腿肌肉内，每只2ml，速度为4ml/h，并在注射前后10min、注射后一周内每天取兔耳中动脉血化验，监测肌酐(CR)、肌酸激酶(CK)、天冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)等项目。观察实验动物基本生命体征，对实验室检查结果进行分析。 2、新西兰大白兔24只，随机分为8组，按照计划分别将0.5ml、1.0ml、1.5ml、2.0ml盐酸溶液分步骤以4ml/h的速度滴注入兔背部特定区域肌肉内，前4组观察术后12h内消融区域的情况；后4组观察术后4周内盐酸溶液消融区域的情况。实验结束时处死动物，取材做病理切片，观察消融范围及其周围炎性反应和纤维化程度。 2、新西兰大白兔8只，随机分为2组并组内编号，第一组肝内注射0.5ml盐酸溶液，第二组肝内注射1.0ml盐酸溶液，分别在注射后1天、1月处死实验动物，取材做病理切片，观察消融范围及其周围炎性反应和纤维化程度。 结果 1.6mol/l盐酸溶液2ml滴注入兔肌肉组织后，兔外周血实验室检查可见肌酐、肌酸激酶、天冬氨酸氨基转移酶、丙氨酸氨基转移酶等指标在术后3天内一过性升高，后逐渐恢复正常。动物一般情况良好，，注射局部皮肤溃烂，呈淡黄色。 2.大体病理结果显示，0.5ml、1.0ml、1.5ml、2.0ml盐酸溶液作用于肌肉，消融最大范围分别为2.77±0.29、3.08±0.75、3.22±0.98、3.46±0.20厘米，约在注射盐酸溶液12h后出现；12h内坏死范围随盐酸剂量的递增、时间延长而不断增大，坏死区域周围可见粒细胞、多核巨细胞聚集；随时间推移，12h-4w消融范围逐渐缩小，3周后坏死组织内可见大量的纤维细胞。 2.8只新西兰大白兔肝内定量注射盐酸溶液后，基本生命体征平稳。定期处死实验动物，取材行病理学分析。大体病理显示切面灰白色，质地均匀；镜下可见盐酸注射区域坏死明显，周围可见炎性反应区域。0.5ml盐酸溶液作用于肝脏组织，消融24h后，消融范围约为2.05cm,1月后约为1.72cm；1.0ml盐酸溶液作用于肝脏组织，消融1月后，消融范围约为2.75cm,1月后约为2.17cm。 结论 1、4只新西兰大白兔经6mol/l盐酸溶液局部注射后，消融效果良好，肌酐、肌酸激酶、天冬氨酸氨基转移酶、丙氨酸氨基转移酶一过性升高，3天后机体经肝脏、肾脏代谢可自行将外周血监测指标调整至正常范围，实验兔基本生命体征平稳。6mol/l盐酸溶液在CT引导下作为消融剂使用安全可靠。 2、24只新西兰大白兔肌肉滴注盐酸溶液后，消融范围内细胞坏死彻底，消融范围在12h内随时间延长而逐渐增大，消融后12小时至4周，消融范围随时间延长而缩小。范围与剂量成正比，消融区域范围可控，坏死彻底。 3、8只新西兰大白兔肝脏滴注6mol/l盐酸溶液后，未见明显全身反应，消融局部区域细胞核溶解，消融范围外1cm区域内汇管区可见坏死，消融范围可控、细胞坏死彻底。
[Abstract]:Purpose 1. The effect of local injection of 6 mol/ l hydrochloric acid solution on the body of New Zealand white rabbits was studied. Sex.2. To study the size of different dose of hydrochloric acid solution on the liver and back muscle of New Zealand white rabbit, to observe the tissue necrosis and the surrounding inflammatory reaction at different stages of the ablation, to define the histological change of the ablation area, and to summarize the body's repair of the ablation area of hydrochloric acid. The law of complex. Materials and Methods 1.6 mol/ l hydrochloric acid solution was slowly injected into the front leg muscles of four New Zealand white rabbits under the guidance of CT, with a speed of 4 ml/ h and 10 min before and after the injection, and the arterial blood test and the monitor in the ear of rabbits were taken every day after the injection. myostatin (CR), creatine kinase (CK), aspartate aminotransferase (AST), alanine aminotransferase ( The items such as ALT were observed. The basic vital signs of the experimental animals were observed, and the laboratory test was performed. The results were analyzed. Twenty-four New Zealand white rabbits were randomly divided into 8 groups, 0.5 ml, 1.0 ml, 1.5 ml, and 2.0 ml of hydrochloric acid solution were injected into the specific area of the back of the rabbit at a rate of 4 ml/ h, respectively. The condition of the ablation area within h;4 weeks after the last 4 weeks, the hydrochloric acid was observed in 4 weeks after the operation The area of the solution was ablated. The animals were sacrificed at the end of the experiment, the materials were obtained for pathological sections, and the ablation range and the surrounding inflammation were observed. Response and fibrosis degree.8 New Zealand white rabbits were randomly divided into 2 groups and group numbers. The first group was injected with 0.5 ml of hydrochloric acid solution, and the second group was injected with 1.0 ml of hydrochloric acid solution respectively. To test the animal, make a pathological section for the materials, and observe the ablation scope and the week pericarditis Results: After 2 ml of 1.6 mol/ l hydrochloric acid solution was injected into the muscle tissue of the rabbit, the blood pressure of the rabbit's peripheral blood was examined and the indexes of creatine kinase, creatine kinase, aspartate aminotransferase and alanine aminotransferase were detected at 3. A transient increase in the day and a gradual return to normal. The animals generally have a good condition. The results showed that 0.5 ml, 1.0 ml, 1.5 ml, and 2.0 ml of hydrochloric acid were applied to the muscles. The maximum range of ablation was 2.77, 0.29, 3.08, 0.75, 3.22, 0.98, 3.46 and 0.20 cm, respectively, and then appeared after the injection of the hydrochloric acid solution for 12 h, and the area of necrosis over 12 h the dose of the hydrochloric acid is increased, the time is prolonged, and the peripheral visible granulocytes and the polynuclear giant cells of the necrotic area are gathered; over time, the ablation range of the 12h-4w is gradually reduced, A large number of fibroblasts were visible in the necrotic tissue after 3 weeks. 2.8 New Zealand white rabbits' liver After the internal quantitative injection of the hydrochloric acid solution, the basic vital signs were flat. Stable. The experimental animals were sacrificed on a regular basis, and the pathological analysis was performed on the materials. The gross pathology showed that the section was off-white and the texture was uniform; the area of the injection of the hydrochloric acid was visible under the microscope, and the surrounding area of the inflammatory reaction was observed. The 0.5 ml of the hydrochloric acid solution was applied to the liver tissue. After the ablation for 24 h, the ablation range was about 2.05 cm, and about 1.72 after 1 month. cm; 1.0 ml of hydrochloric acid solution was applied to the liver tissue, after 1 month of ablation, the ablation range was approximately 2 Conclusion 1. After local injection of 6 mol/ l hydrochloric acid solution,4 New Zealand white rabbits have good ablation effect, muscle strength, creatine kinase, and aspartate aminotransferase. Alanine aminotransferase increased transiently. After 3 days, the body had liver and kidney metabolism to adjust the peripheral blood monitoring index to the normal range, and the basic vital signs of the experimental rabbits were stable. / l Hydrochloric acid solution was safe and reliable as an ablation agent under the guidance of CT. Large,12-to-4-week post-ablation, and the ablation range extended over time The scope was in direct proportion to the dose, the area of the ablation was controlled and the necrosis was complete.3,8 New Zealand white rabbits were instilled with 6 mol/ l of hydrochloric acid in the liver, no obvious systemic reaction was observed, the nuclei of the local area of the ablation were dissolved, and the ablation scope was out of scope.
【学位授予单位】：中国人民解放军医学院
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R814.42
本文编号：2483807
[Abstract]:Purpose 1. The effect of local injection of 6 mol/ l hydrochloric acid solution on the body of New Zealand white rabbits was studied. Sex.2. To study the size of different dose of hydrochloric acid solution on the liver and back muscle of New Zealand white rabbit, to observe the tissue necrosis and the surrounding inflammatory reaction at different stages of the ablation, to define the histological change of the ablation area, and to summarize the body's repair of the ablation area of hydrochloric acid. The law of complex. Materials and Methods 1.6 mol/ l hydrochloric acid solution was slowly injected into the front leg muscles of four New Zealand white rabbits under the guidance of CT, with a speed of 4 ml/ h and 10 min before and after the injection, and the arterial blood test and the monitor in the ear of rabbits were taken every day after the injection. myostatin (CR), creatine kinase (CK), aspartate aminotransferase (AST), alanine aminotransferase ( The items such as ALT were observed. The basic vital signs of the experimental animals were observed, and the laboratory test was performed. The results were analyzed. Twenty-four New Zealand white rabbits were randomly divided into 8 groups, 0.5 ml, 1.0 ml, 1.5 ml, and 2.0 ml of hydrochloric acid solution were injected into the specific area of the back of the rabbit at a rate of 4 ml/ h, respectively. The condition of the ablation area within h;4 weeks after the last 4 weeks, the hydrochloric acid was observed in 4 weeks after the operation The area of the solution was ablated. The animals were sacrificed at the end of the experiment, the materials were obtained for pathological sections, and the ablation range and the surrounding inflammation were observed. Response and fibrosis degree.8 New Zealand white rabbits were randomly divided into 2 groups and group numbers. The first group was injected with 0.5 ml of hydrochloric acid solution, and the second group was injected with 1.0 ml of hydrochloric acid solution respectively. To test the animal, make a pathological section for the materials, and observe the ablation scope and the week pericarditis Results: After 2 ml of 1.6 mol/ l hydrochloric acid solution was injected into the muscle tissue of the rabbit, the blood pressure of the rabbit's peripheral blood was examined and the indexes of creatine kinase, creatine kinase, aspartate aminotransferase and alanine aminotransferase were detected at 3. A transient increase in the day and a gradual return to normal. The animals generally have a good condition. The results showed that 0.5 ml, 1.0 ml, 1.5 ml, and 2.0 ml of hydrochloric acid were applied to the muscles. The maximum range of ablation was 2.77, 0.29, 3.08, 0.75, 3.22, 0.98, 3.46 and 0.20 cm, respectively, and then appeared after the injection of the hydrochloric acid solution for 12 h, and the area of necrosis over 12 h the dose of the hydrochloric acid is increased, the time is prolonged, and the peripheral visible granulocytes and the polynuclear giant cells of the necrotic area are gathered; over time, the ablation range of the 12h-4w is gradually reduced, A large number of fibroblasts were visible in the necrotic tissue after 3 weeks. 2.8 New Zealand white rabbits' liver After the internal quantitative injection of the hydrochloric acid solution, the basic vital signs were flat. Stable. The experimental animals were sacrificed on a regular basis, and the pathological analysis was performed on the materials. The gross pathology showed that the section was off-white and the texture was uniform; the area of the injection of the hydrochloric acid was visible under the microscope, and the surrounding area of the inflammatory reaction was observed. The 0.5 ml of the hydrochloric acid solution was applied to the liver tissue. After the ablation for 24 h, the ablation range was about 2.05 cm, and about 1.72 after 1 month. cm; 1.0 ml of hydrochloric acid solution was applied to the liver tissue, after 1 month of ablation, the ablation range was approximately 2 Conclusion 1. After local injection of 6 mol/ l hydrochloric acid solution,4 New Zealand white rabbits have good ablation effect, muscle strength, creatine kinase, and aspartate aminotransferase. Alanine aminotransferase increased transiently. After 3 days, the body had liver and kidney metabolism to adjust the peripheral blood monitoring index to the normal range, and the basic vital signs of the experimental rabbits were stable. / l Hydrochloric acid solution was safe and reliable as an ablation agent under the guidance of CT. Large,12-to-4-week post-ablation, and the ablation range extended over time The scope was in direct proportion to the dose, the area of the ablation was controlled and the necrosis was complete.3,8 New Zealand white rabbits were instilled with 6 mol/ l of hydrochloric acid in the liver, no obvious systemic reaction was observed, the nuclei of the local area of the ablation were dissolved, and the ablation scope was out of scope.
【学位授予单位】：中国人民解放军医学院
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R814.42
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