间歇运动对卵巢切除大鼠脊髓背角内吗啡肽2表达的影响

发布时间：2019-05-31 18:37

【摘要】：目的:探讨间歇运动对卵巢切除(ovariectomized,OVX)大鼠脊髓背角内吗啡肽2(endomorphin-2,EM-2)表达的影响。方法:体重180~200 g雌性SD大鼠,随机分为假手术组(Sham)、卵巢切除安静组(OVX)、卵巢切除+间歇运动组(OVXex),每组8只。建立模拟女性绝经后状况的卵巢切除模型,OVXex采用小动物跑台进行7周间歇训练。每周训练结束后次日,对各组大鼠进行热异常痛行为学检测,评定大鼠卵巢切除后的痛敏状况;7周训练结束后次日取腰膨大处脊髓背角,切片并进行EM-2免疫组织化学染色,检测其表达。结果:卵巢切除引起了明显的热异常痛敏,同时EM-2表达明显降低。间歇运动可有效改善热异常痛敏,同时上调脊髓EM-2的表达。结论:间歇运动可显著增加脊髓背角EM-2的表达,减弱卵巢切除引起的热异常痛敏。运动干预卵巢切除所引起的脊髓背角EM-2表达和热痛行为学反应的改变,将为绝经后女性的异常性痛敏提供治疗的新思路。
[Abstract]:Aim: to investigate the effect of intermittent exercise on the expression of morphine 2 (endomorphin-2,EM-2) in spinal dorsal horn of ovariectomy (ovariectomized,OVX) rats. Methods: female SD rats weighing 180 g / 200 g were randomly divided into 8 rats in each group with (Sham), ovariectomy and rest (OVX), ovariectomy intermittent exercise group. An ovariectomy model was established to simulate the postmenopausal status of women. OVXex used small animal treadmill for 7 weeks of intermittent training. On the second day after weekly training, the rats in each group were tested for abnormal heat pain, and the hyperalgesia status after ovariectomy was evaluated. The spinal dorsal horn of lumbar enlargement was taken the next day after 7 weeks of training, and the expression of spinal dorsal horn was detected by EM-2 histochemical staining. Results: ovariectomy caused obvious abnormal hyperalgesia and decreased the expression of EM-2. Intermittent exercise can effectively improve thermal abnormal hyperalgesia and up-regulate the expression of EM-2 in spinal cord. Conclusion: intermittent exercise can significantly increase the expression of EM-2 in spinal dorsal horn and weaken the abnormal hyperalgesia caused by ovariectomy. Exercise intervention in spinal dorsal horn EM-2 expression and thermal pain behavioral response induced by ovariectomy will provide a new idea for the treatment of abnormal pain sensitivity in postmenopausal women.
【作者单位】：西安建筑科技大学体育系;
【基金】：西安建筑科技大学择优立项课程建设项目
【分类号】：G804.5

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